Mos 3′ UTR regulatory differences underlie species‐specific temporal patterns of Mos mRNA cytoplasmic polyadenylation and translational recruitment during oocyte maturation

Prasad, C. Krishna; Mahadevan, Maha M.; MacNicol, Melanie C.; MacNicol, Angus M.

doi:10.1002/mrd.20877

Cited by 21 publications

(19 citation statements)

References 63 publications

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“…However, all such candidate CPE‐dependant mRNAs that we have so far examined are translationally activated late, coincident with the completion of GVBD (e.g. cyclin B2 (Figure 1A), a synthetic reporter containing a single ‘early’ CPE (Figure 1B) or a reporter mRNA fused to the CPE‐dependant human Mos 3′ UTR (Prasad et al , 2008)). Furthermore, some established early class mRNAs lack a functional CPE in their 3′ UTRs (e.g.…”

Section: Discussionmentioning

confidence: 99%

Enforcing temporal control of maternal mRNA translation during oocyte cell-cycle progression

Arumugam

Wang

Hardy

et al. 2009

EMBO J

100

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Meiotic cell-cycle progression in progesterone-stimulated Xenopus oocytes requires that the translation of pre-existing maternal mRNAs occur in a strict temporal order. Timing of translation is regulated through elements within the mRNA 3 0 untranslated region (3 0 UTR), which respond to cell cycle-dependant signalling. One element that has been previously implicated in the temporal control of mRNA translation is the cytoplasmic polyadenylation element (CPE). In this study, we show that the CPE does not direct early mRNA translation. Rather, early translation is directed through specific early factors, including the Musashi-binding element (MBE) and the MBE-binding protein, Musashi. Our findings indicate that although the cyclin B5 3 0 UTR contains both CPEs and an MBE, the MBE is the critical regulator of early translation. The cyclin B2 3 0 UTR contains CPEs, but lacks an MBE and is translationally activated late in maturation. Finally, utilizing antisense oligonucleotides to attenuate endogenous Musashi synthesis, we show that Musashi is critical for the initiation of early class mRNA translation and for the subsequent activation of CPE-dependant mRNA translation.

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Section: Discussionmentioning

confidence: 99%

Enforcing temporal control of maternal mRNA translation during oocyte cell-cycle progression

Arumugam

Wang

Hardy

et al. 2009

EMBO J

100

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“…This is a conflicting result from previous findings on the translational effect of Msi1. However, in human oocytes, a parallel physiological phenomenon is controlled by other machinery instead of the Musashi homolog proteins (66). In other words, although Musashi family proteins are highly conserved among vertebrates, their precise roles might be species-dependent.…”

Section: Current Insights Into the Functions Of Msi1mentioning

confidence: 99%

The Musashi family RNA-binding proteins in stem cells

Horisawa

Imai

Yanagawa

2010

BioMolecular Concepts

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The Musashi family is an evolutionarily conserved group of RNA-binding proteins. In mammal, two members of the group, Msi1 and Msi2, have been identified to date. Msi1 is considered to play roles in maintaining the stem cell status (stemness) of neural stem/progenitor cells in adults and in the development of central nervous system through translational regulation of its target mRNAs, which encode regulators of signal transduction and the cell cycle. Recently, strong expression of Msi1 in various somatic stem/progenitor cells of adult tissues, such as eye, gut, stomach, breast, and hair follicle, has been reported. The protein is also expressed in various cancer cells, and ectopically emerging cells have been found in neural tissues of patients with diseases involving neural disorder, including epilepsy. Many novel target mRNAs and regulatory pathways of Msi1 have been reported in recent years. Here, we present a review of the functions and action mechanisms of Msi1 protein and discuss possible directions for further study.

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“…Additionally, mammalian MOS harbors CPEs that slightly differ from previously reported canonical and noncanonical CPEs, which we termed CPE‐MamMOS and were also conserved in cattle (Figure ). Regardless, mutational disruption of the nonfunctional CPE (incapable of binding CPEB1) does promote more extensive polyadenylation suggesting it has a role in primates . In mammals, MOS translation occurs at the time of GVBD, which is later compared to Xenopus, where it occurs before GVBD.…”

Section: Individual Genes Regulated By Cp Activity In Mammalian Oocytesmentioning

confidence: 99%

Cytoplasmic polyadenylation in mammalian oocyte maturation

Reyes

Ross

2015

WIREs RNA

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Oocyte developmental competence is the ability of the mature oocyte to be fertilized and subsequently drive early embryo development. Developmental competence is acquired by completion of oocyte maturation, a process that includes nuclear (meiotic) and cytoplasmic (molecular) changes. Given that maturing oocytes are transcriptionally quiescent (as are early embryos), they depend on post-transcriptional regulation of stored transcripts for protein synthesis, which is largely mediated by translational repression and deadenylation of transcripts within the cytoplasm, followed by recruitment of specific transcripts in a spatiotemporal manner for translation during oocyte maturation and early development. Motifs within the 3' untranslated region (UTR) of messenger RNA (mRNA) are thought to mediate repression and downstream activation by their association with binding partners that form dynamic protein complexes that elicit differing effects on translation depending on cell stage and interacting proteins. The cytoplasmic polyadenylation (CP) element, Pumilio binding element, and hexanucleotide polyadenylation signal are among the best understood motifs involved in CP, and translational regulation of stored transcripts as their binding partners have been relatively well-characterized. Knowledge of CP in mammalian oocytes is discussed as well as novel approaches that can be used to enhance our understanding of the functional and contributing features to transcript CP and translational regulation during mammalian oocyte maturation. WIREs RNA 2016, 7:71-89. doi: 10.1002/wrna.1316 For further resources related to this article, please visit the WIREs website.

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Mos 3′ UTR regulatory differences underlie species‐specific temporal patterns of Mos mRNA cytoplasmic polyadenylation and translational recruitment during oocyte maturation

Cited by 21 publications

References 63 publications

Enforcing temporal control of maternal mRNA translation during oocyte cell-cycle progression

Enforcing temporal control of maternal mRNA translation during oocyte cell-cycle progression

The Musashi family RNA-binding proteins in stem cells

Cytoplasmic polyadenylation in mammalian oocyte maturation

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