Movement protein-derived resistance to triple gene block-containing plant viruses.

Abstract

The efficiency of cell-to-cell virus movement is important in determining pathogenicity, virulence and, in some cases, the host range of a plant virus (reviewed by Atabekov & Taliansky, 1990 ;Maule, 1991). When the efficiency of the transport function and the rate of virus movement are reduced, the plant acquires a certain level of resistance to virus infection.Production of dysfunctional or partially active movement proteins (MP) in transgenic plants is assumed to confer resistance to the wild-type (wt) virus by competition between wt virus-coded MP and the preformed modified MP (mMP). In support of this assumption, it has been reported that transgenic tobacco plants which produce a non-functional MP of tobacco mosaic virus (TMV) acquire resistance to TMV infection (Malyshenko et al., 1993 ;Lapidot et al., 1993). In addition, transgenic plants expressing non-functional TMV MP were resistant to several distantly related or unrelated viruses (Lapidot et al., 1993 ;Cooper et al., 1995). These results suggest

Triple gene block: modular design of a multifunctional machine for plant virus movement

“…they inhibit cell-to-cell movement and diminish virus accumulation (Beck et al, 1994;Seppanen et al, 1997;Lauber et al, 2001). The recently discovered ability of potex-and hordei-like TGBp2 to facilitate movement of GFP between adjacent epidermal cells (Tamai & Meshi, 2001; our unpublished data) suggests that co-expression of a non-functional TGBp2 mutant during infection can interfere not only with viral RNP trafficking to PD but also with some additional movementrelated function(s).…”

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

“…Kemira also collaborated with Moscow State University to develop broadspectrum, pathogen-derived resistance to several viruses in potato using a modified viral movement protein gene as the transgene. The resistance was thought to be protein-mediated (Seppänen et al 1997). At the same time at the former Department of Plant Production (now part of DAB) in AF-UH, potato cv.…”

Transgenic Resistance

“…For example, transgenic expression of defective-mutant 30-kDa movement protein of TMV in tobacco (Lapidot et al, 1993;Malyshenko et al, 1993), 12-kDa movement protein of PVX in potato (Seppänen et al, 1997), and 13-kDa movement protein of White clover mosaic virus (WClMV) in N. benthamiana (Beck et al, 1994), resulted in the inhibition of eponymous virus infection, as well as infection by viruses in other genera and sometimes families (Beck et al, 1994;Cooper, Lapidot, Heick, Dodds, & Beachy, 1995). For example, transgenic expression of defective-mutant 30-kDa movement protein of TMV in tobacco (Lapidot et al, 1993;Malyshenko et al, 1993), 12-kDa movement protein of PVX in potato (Seppänen et al, 1997), and 13-kDa movement protein of White clover mosaic virus (WClMV) in N. benthamiana (Beck et al, 1994), resulted in the inhibition of eponymous virus infection, as well as infection by viruses in other genera and sometimes families (Beck et al, 1994;Cooper, Lapidot, Heick, Dodds, & Beachy, 1995).…”