MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets

Stockert, Juan C.; Blázquez‐Castro, Alfonso; Cañete, Magdalena; Horobin, Richard W.; Villanueva, Ángeles

doi:10.1016/j.acthis.2012.01.006

Cited by 518 publications

(338 citation statements)

References 71 publications

Supporting

Mentioning

312

Contrasting

Unclassified

Order By: Relevance

“…The intracellular reducing power is mainly provided by NAD(P)H which is derived from dehydrogenase activity in mitochondria, endoplasmic reticulum and plasma membrane ( Stockert et al, 2012). …”

Section: Mtt Reduction Cytotoxic Assay For Evaluation Of Medicinal Plmentioning

confidence: 99%

Growth Inhibition and Induction of Apoptosis in Different Carcinoma Cell Lines by Euphorbia tirucalli Stem Extracts

Joshi¹,

Mandavia²,

I.A.³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Section: Mtt Reduction Cytotoxic Assay For Evaluation Of Medicinal Plmentioning

confidence: 99%

Growth Inhibition and Induction of Apoptosis in Different Carcinoma Cell Lines by Euphorbia tirucalli Stem Extracts

Joshi¹,

Mandavia²,

I.A.³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…Optimization of culture Conditions of Selected Isolate:-Optimization of culture conditions for enhancing metabolite antiviral Activity:-The antiviral activity was enhanced by manipulating selected physical parameters, whereas pH levels of the basal media were adjusted from 5 to 10, followed by adjusting the optimum temperature for growth ranging from 20˚C to 50˚C and finally the effect of incubation period (Stockert et al, 2012).…”

Section: Macroscopic and Microscopic Identification Of The Isolate:-mentioning

confidence: 99%

Antiviral Activity of Secondary Metabolites Produced by Streptomyces Species Isolated From Egyptian Soil.

Gaied¹

2017

IJAR

View full text Add to dashboard Cite

“…Medium containing the compound was changed every 2 days. The cell viability was measured by MTT assay (Stockert, Blazquez-Castro, Canete, Horobin, & Villanueva, 2012), and the cytotoxic response of RAW264.7 was evaluated by trypan blue exclusion (Shih, Kuo, Chuang, Cheng, & Doong, 2000). The 50% and 10% inhibitory concentrations of the compound were calculated using the formula: %IC = [1-(A 570test /A 570cont )] × 100, where % IC = % inhibition of cell proliferation, A 570test = absorbance of test sample, A 570cont = absorbance of control sample (Cetin & Bullerman, 2005).…”

Section: Ic 10 and Ic 50 Determinationmentioning

confidence: 99%

Suppression of Hepatitis B Virus Production and Inflammatory Response in vitro and in vivo by Mormodica charantia Compound EMCDO

Chang¹,

Chen²,

Chen³

et al. 2015

JAS

View full text Add to dashboard Cite

Eight compounds were purified from Mormodica charanti, and their chemical structures were determined in this study. Their anti-HBV and anti-inflammation activities were investigated. Compound EMCDO exhibited the most efficient effect in terms of reducing HBV surface antigen, e antigen and viral DNA levels in HBV particles or surface antigen-producing cells 2.2.15 and PLC/PRF/5, respectively. Tumor suppressor p53 played a significant role in EMCDO-mediated anti-HBV effects. Pretreatment with EMCDO prevented 2.2.15 cells-induced tumor formation in a nude mice subcutaneous model. The anti-HBV and anti-tumor activities of EMCDO were better than those of oltipraz, an inhibitor of HBV transcription. EMCDO reduced the proinflammatory cytokines and mediators in LPS-treated RAW264.7 cells in a dose-dependent manner. The LPS-upregulated phosphorylation level of I was reduced in the presence of EMCDO. The transcription activity of NF-B was increased in cells treated with EMCDO. Utilization of a mouse ear edema model further confirmed the activity of EMCDO against TPA-elicited inflammation.

show abstract

MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets

Cited by 518 publications

References 71 publications

Growth Inhibition and Induction of Apoptosis in Different Carcinoma Cell Lines by Euphorbia tirucalli Stem Extracts

Growth Inhibition and Induction of Apoptosis in Different Carcinoma Cell Lines by Euphorbia tirucalli Stem Extracts

Antiviral Activity of Secondary Metabolites Produced by Streptomyces Species Isolated From Egyptian Soil.

Suppression of Hepatitis B Virus Production and Inflammatory Response in vitro and in vivo by Mormodica charantia Compound EMCDO

Contact Info

Product

Resources

About