Mucoralean fungi for sustainable production of bioethanol and biologically active molecules

Satari, Behzad; Karimi, Keikhosro

doi:10.1007/s00253-017-8691-9

Cited by 37 publications

(14 citation statements)

References 207 publications

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“…For example, fungal enzyme cocktails containing cellulases, hemicellulases, and ligninases are used to convert waste plant material to fermentable sugars, which are subsequently used as substrates in biofuel production with an estimated annual value of over $4.5 billion [1]. In addition, there is growing interest in the use of filamentous fungi for bioethanol fermentation due to their capability to grow on a wide range of sugars, and their high tolerance to many inhibitory molecules produced from hydrolysed plant biomass [5–7].…”

Section: Introductionmentioning

confidence: 99%

A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger

Cairns

Feurstein

Zheng

et al. 2019

Biotechnol Biofuels

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Background Fungal fermentation is used to produce a diverse repertoire of enzymes, chemicals, and drugs for various industries. During submerged cultivation, filamentous fungi form a range of macromorphologies, including dispersed mycelia, clumped aggregates, or pellets, which have critical implications for rheological aspects during fermentation, gas/nutrient transfer, and, thus, product titres. An important component of strain engineering efforts is the ability to quantitatively assess fungal growth phenotypes, which will drive novel leads for morphologically optimized production strains. Results In this study, we developed an automated image analysis pipeline to quantify the morphology of pelleted and dispersed growth (MPD) which rapidly and reproducibly measures dispersed and pelleted macromorphologies from any submerged fungal culture. It (i) enables capture and analysis of several hundred images per user/day, (ii) is designed to quantitatively assess heterogeneous cultures consisting of dispersed and pelleted forms, (iii) gives a quantitative measurement of culture heterogeneity, (iv) automatically generates key Euclidian parameters for individual fungal structures including particle diameter, aspect ratio, area, and solidity, which are also assembled into a previously described dimensionless morphology number MN, (v) has an in-built quality control check which enables end-users to easily confirm the accuracy of the automated calls, and (vi) is easily adaptable to user-specified magnifications and macromorphological definitions. To concomitantly provide proof of principle for the utility of this image analysis pipeline, and provide new leads for morphologically optimized fungal strains, we generated a morphological mutant in the cell factory Aspergillus niger based on CRISPR-Cas technology. First, we interrogated a previously published co-expression networks for A. niger to identify a putative gamma-adaptin encoding gene ( aplD ) that was predicted to play a role in endosome cargo trafficking. Gene editing was used to generate a conditional aplD expression mutant under control of the titratable Tet-on system. Reduced aplD expression caused a hyperbranched growth phenotype and diverse defects in pellet formation with a putative increase in protein secretion. This possible protein hypersecretion phenotype could be correlated with increased dispersed mycelia, and both decreased pellet diameter and MN. Conclusion The MPD image analysis pipeline is a simple, rapid, and flexible approach to quantify diverse fungal morphologies. As an exemplar, we have demonstrated that the putative endosomal transport gene aplD plays a crucial role in A. niger filamentous growth and pellet formation during submerged culture. This suggests that endocytic components are ...

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Section: Introductionmentioning

confidence: 99%

A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger

Cairns

Feurstein

Zheng

et al. 2019

Biotechnol Biofuels

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“…Utilization of agro-industrial residues for the manufacturing of mycoprotein is another strategy that can be considered to decrease the environmental impact of this meat substitute. Lignocellulosic materials without pretreatment can be used by filamentous fungus for the production of mycoprotein in submerged culture as well as in solid-state fermentation (Satari and Karimi 2018). The challenge in this alternative is to find agro-industrial waste streams that have beneficial nutritional composition to guarantee an efficient production.…”

Section: Introductionmentioning

confidence: 99%

Mycoprotein: environmental impact and health aspects

Filho

Andersson

Ferreira

et al. 2019

World J Microbiol Biotechnol

115

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The term mycoprotein refers to the protein-rich food made of filamentous fungal biomass that can be consumed as an alternative to meat. In this paper, the impact caused by the substitution of animal-origin meat in the human diet for mycoprotein on the health and the environment is reviewed. Presently, mycoprotein can be found in the supermarkets of developed countries in several forms (e.g. sausages and patties). Expansion to other markets depends on the reduction of the costs. Although scarce, the results of life cycle analyses of mycoprotein agree that this meat substitute causes an environmental impact similar to chicken and pork. In this context, the use of inexpensive agro-industrial residues as substrate for mycoprotein production has been investigated. This strategy is believed to reduce the costs involved in the fungal cultivation and lower the environmental impact of both the mycoprotein and the food industry. Moreover, several positive effects in health have been associated with the substitution of meat for mycoprotein, including improvements in blood cholesterol concentration and glycemic response. Mycoprotein has found a place in the market, but questions regarding the consumer’s experience on the sensory and health aspects are still being investigated.

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“…Species of M. ramosissimus produce extracellular enzymes, such as endopolygalacturonase and lipase, and secondary metabolites, such as phytoalexin elicitor [42–44]. Recently, several studies have focused on applying Mucorales members to produce ethanol and biomass by-product [45]. Particularly, M. ramosissimus has been reported as a potential ethanol-producing mold [46].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Three Zygomycetous Fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus

Nguyen

Lee

2018

Mycobiology

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While surveying undiscovered fungal taxa in Korea, three rare zygomycetous fungal strains, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were isolated from soil, leaf, and freshwater samples, respectively. The strains were analyzed morphologically as well as phylogenetically based on the internal transcribed spacer region and 28S rDNA sequences. Sequence analysis of the two loci revealed that the isolates, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were identified as Backusella circina, Circinella muscae, and Mucor ramosissimus, respectively. These species have not yet been previously described in Korea.

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Mucoralean fungi for sustainable production of bioethanol and biologically active molecules

Cited by 37 publications

References 207 publications

A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger

A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger

Mycoprotein: environmental impact and health aspects

Isolation and Characterization of Three Zygomycetous Fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus

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