Mucosal gastrin concentration, molecular forms of gastrin, number and ultrastructure of G-cells in patients with duodenal ulcer.

Creutzfeldt, W.; Arnold, R.; Creutzfeldt, C.; Track, Norman S.

doi:10.1136/gut.17.10.745

Cited by 115 publications

(45 citation statements)

References 23 publications

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“…Concerning the subcellular mechanism of gastrin release, although our experimental protocols were not exactly comparable, we did not see the striking changes in gastrin granule electron density reported to occur on fasting and refeeding rats which could suggest a release of gastrin from the granules into the cytoplasm (Creutzfeldt et al, 1975(Creutzfeldt et al, , 1976. This demonstration of a correlation between the immunoreactive gastrin content of the rat antrum and the number of G cell granules suggests that entire granules are lost, possibly by exocytosis.…”

Section: Discussionmentioning

confidence: 58%

Gastrin and the ultrastructure of G cells in the fasting rat.

Mortensen¹,

Morris²,

Owens³

1979

Gut

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SUMMARY The effect of fasting on serum and antral gastrin concentrations and G cell ultrastructure in the rat has been examined using a radioimmunoassay and quantitative electron microscopy. Serum gastrin levels in fasting animals were markedly reduced and there was also a significant decrease in antral gastrin concentrations after 48 hours and 72 hours of fasting. This was associated with a significant fall in the granule content and cytoplasmic volume of individual G cells, at its greatest by 48 hours. A relative absence of electron dense granules in the Golgi zones of cells from animals fasted for 72 hours suggested a paucity of newly formed granules, but fasting produced no detectable change in the electron density of the granule population taken as a whole. The results indicate that, during fasting, release and then synthesis of gastrin is inhibited, so that granule stores and cell size diminish. The correlation between the granule content of G cells and the antral content of gastrin suggests that hormone release occurs by exocytosis, rather than by any change in the content of individual granules.

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Section: Discussionmentioning

confidence: 58%

Gastrin and the ultrastructure of G cells in the fasting rat.

Mortensen¹,

Morris²,

Owens³

1979

Gut

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“…The G cell distribution was determined by indirect immunohistochemical assay using an antigastrin antibody described by Yanaihara et al (1980), and the G cell density was measured according to the method of Creutzfeldt et al (1976). Five serial sections of prepylorus antral mucosa were prepared from surgical specimens obtained from patients who required re-resection for recurrent disease, and endoscopic biopsy specimens from patients without recurrence ; surgical specimens from patients with early gastric cancer, matched for age, were used as a control group.…”

Section: Questionnaire and Interviewmentioning

confidence: 99%

Long-Term Results of Pylorus-Preserving Gastrectomy for Gastric Ulcer.

Sasaki

Fukushima

Naitö

et al. 1992

Tohoku J. Exp. Med.

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The postoperative results of pylorus-preserving gastrectomy (PPG) for gastric ulcer performed in 134 patients during the past 25 years (mean postoperative period, 16.6 years) were studied. The incidence of postoperative complications was low. Dumping syndrome occurred in only 4.4% and 0% of cases as assessed by questionnaire and interview, respectively. Four (5.4%) of 74 patients available for this study had ulcer recurrence. In one of these four patients concurrent gastroduodenal ulcer was suspected from preoperative gastric analysis. The site of recurrence was found in all cases to be the remnant antral gland area along the greater curvature between the proper gastric gland area and the duodenum. Basal and maximal acid outputs at the time of relapse were significantly higher in patients with recurrence than in patients without recurrence. The fasting and postprandial serum gastrin levels were high in one patient with recurrence, whose antrum was preserved as long as 3 cm proximal to the pyloric ring ; this was longer than that described in our original method of PPG. In two other recurrent cases the serum gastrin levels were not different from those in norecurrent cases. Immunohistochemical examination of the residual antrum showed no increase in the G-cell density in patients either with or without recurrence. These results suggest that the long-term quality of life of patients treated with PPG remains favorable. Recurrence rate can be further reduced if PPG is strictly indicated for gastric ulcer only and carried out by meticulous surgical techniques. In the pathogenesis of the ulcer recurrence the role of gastrin release from the residual antral mucosa seems to be limited. pylorus-preserving gastrectomy ; long-term follow-up ; postoperative ulcer recurrence Since conventional gastrectomy involves pyloric resection, postoperative sequelae, such as dumping syndrome and other disorders of digestion and absorption may occur as a result of rapid gastric emptying. To minimize these complications, we have performed pylorus-preserving gastrectomy (PPG) for

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“…Im munoreactive gastrin (IRG) was estimated by radio immunoassay (10,14) and protein content by the Lowry method. Tissue IRG concentrations were ex pressed as nanograms of gastrin per milligram of protein.…”

Section: Tissue Preparation For Immunoreactive Gastrin Measurementmentioning

confidence: 99%

“…The stomach, duodenum and when possible the pancreas were removed; when size permitted, the stomach was divided into three portions. Adult antral and duodenal mucosal specimens were collected as described previ ously (10).…”

Section: Tissue Sourcesmentioning

confidence: 99%

Appearance of Gastrin and Somatostatin in the Human Fetal Stomach, Duodenum and Pancreas

et al. 1979

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The gestational time of appearance of gastrin and somatostatin in the human fetal stomach, duodenum and pancreas was examined. Immunoreactive gastrin (IRG) is detected in antral, duodenal and pancreatic extracts of a 7.0-cm (crown-heel length) fetus. More IRG is extracted from the duodenum than the antrum. Duodenal IRG concentrations from fetuses of 16.0–26.0 cm are higher than younger fetal and adult concentrations. Antral IRG concentrations are one tenth of the adult contents. Very small IRG concentrations are present in the human fetal pancreas. Gastrin immunohistochemical staining is positive first in duodenal (6.5-cm fetus) and later in antral (12.5-cm fetus) mucosa; pancreatic tissue is negative for gastrin immunohistochemistry. Type IV cells are encountered in antral and duodenal mucosa of 4.0-cm fetuses; other endocrine cells appear with fetal growth. Not until much later in gestation (21.0 cm) do typical G cells appear. These results suggest that early in fetal life gastrin is produced by the type IV cell. Somatostatin immunohistochemical staining is positive in stomach, duodenum and pancreas in 6.5-cm fetuses. Immature D cells are found in antral and duodenal mucosa of 5.0-cm fetuses and mature D cells in 11.0-cm fetuses.

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Mucosal gastrin concentration, molecular forms of gastrin, number and ultrastructure of G-cells in patients with duodenal ulcer.

Cited by 115 publications

References 23 publications

Gastrin and the ultrastructure of G cells in the fasting rat.

Gastrin and the ultrastructure of G cells in the fasting rat.

Long-Term Results of Pylorus-Preserving Gastrectomy for Gastric Ulcer.

Appearance of Gastrin and Somatostatin in the Human Fetal Stomach, Duodenum and Pancreas

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