2014
DOI: 10.1016/j.jviromet.2014.08.025
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Multi-colored immunochromatography using nanobeads for rapid and sensitive typing of seasonal influenza viruses

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Cited by 24 publications
(20 citation statements)
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“…5 NanoAct labels (Asahi Kasei, Miyazaki, Japan) are cellulose nanobeads that were reported to yield 10-fold higher sensitivity than gold or latex labels in an influenza LFA. 37 The authors attribute the improved sensitivity to the larger surface area of the NanoAct labels (330 nm), and more intense dye colours available for impregnation into the cellulosic material. Carbon nanoparticle labels were reported to improve sensitivity 100-fold over gold label in a dengue antigen NS1 LFA.…”
Section: Colorimetric Labelsmentioning
confidence: 99%
“…5 NanoAct labels (Asahi Kasei, Miyazaki, Japan) are cellulose nanobeads that were reported to yield 10-fold higher sensitivity than gold or latex labels in an influenza LFA. 37 The authors attribute the improved sensitivity to the larger surface area of the NanoAct labels (330 nm), and more intense dye colours available for impregnation into the cellulosic material. Carbon nanoparticle labels were reported to improve sensitivity 100-fold over gold label in a dengue antigen NS1 LFA.…”
Section: Colorimetric Labelsmentioning
confidence: 99%
“…Analyte quantification is challenging as a result of variations in lighting, sample volume, and the operator's visual acuity. Despite numerous advancements in colorimetric labels, including gold nanocages [1], nanotubes [2], carbon nanoparticles [3], and cellulose nanobeads [4], among others, the sensitivity, signal range, and quantifiability of colorimetric label based LFAs remain a challenge. The use of other optical labels such as fluorescent dyes [5,6] or luminescent nanoparticles [7,8] faces various challenges due to reagent complexity, cost, and reader complexity [9].…”
Section: Of 11mentioning
confidence: 99%
“…Clinical samples applied to LFAs range from urine in a pregnancy test [16] or a TB LFA [8], plasma or serum for HIV detection [4], whole blood for Dengue fever diagnosis [17] and nasal swabs for flu detection [18]. These varied clinical samples may be quite similar from patient to patient (pH of serum) or quite different for an individual patient, throughout the day (urine).…”
Section: Lfa Components and Analytical Tools For Characterizationmentioning
confidence: 99%
“…While this deector configuration is a staple of many commercial LFA tests, the optimal preparation conditions vary with each antibody and may vary from colloidal gold vendor to vendor. Other detector particles include up-converting phosphors [35], fluorescent molecules [9] or other nanoparticles, such as cellulose nanobeads [18], latex beads [36] or paramagnetic beads [37]. The analytical tools described below (Table 2) can be applied to both conjugated and unconjugated nanoparticles and used to optimize different conjugation strategies.…”
Section: Lfa Components and Analytical Tools For Characterizationmentioning
confidence: 99%