Multi-functional egg white hydrolysate prevent hypertension and vascular dysfunction induced by cadmium in rats

Moraes, Paola Zambelli; Júnior, José Eudes Gomes Pinheiro; Martinez, Caroline Silveira; Moro, Camila Rodrigues; Silva, Grazielly Caroline da; Rodriguez, Marina Diaz; Barbosa, Fernando; Peçanhã, Franck Maciel; Vassallo, Dalton Valentim; Miguel, Marta; Wiggers, Giulia Alessandra

doi:10.1016/j.jff.2022.105131

Cited by 6 publications

(7 citation statements)

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“…In the present study, the improvement in ACh-induced endothelium-dependent relaxation in MRA and aorta of the DOCA-salt rats supplemented with EWH was due to the increase in endothelial function, as NO donor did not change its response among groups. The present results agree with previous results using models of exposure to different metals, in which the protective effect of EWH on the endothelium was observed in resistance and conductance vessels [ 21 , 22 , 23 , 24 ].…”

Section: Discussionsupporting

confidence: 93%

“…Previously our group demonstrated that EWH has antihypertensive, vasorelaxant, antioxidant, and anti-inflammatory properties, observed in a genetic model of hypertension SHR and metals-induced hypertension [ 15 , 21 , 22 , 23 , 24 ]. In this work, for the first time, we show a significant antihypertensive effect of EWH in a model of severe hypertension induced by DOCA-salt treatment in uninephrectomized rats.…”

Section: Discussionmentioning

confidence: 99%

“…However, in this metal’s exposure models, the blood pressure levels were within normal limits [ 21 , 23 ] or in stage 1 of hypertension [ 2 , 22 ]. Moreover, in rats exposed to high concentrations of cadmium, which showed hypertension at high levels, EWH also reversed high blood pressure levels towards those of the control animals [ 24 ]. An essential point on which those results differ from those observed in the present study is that in animals exposed to toxic metals, the treatment was performed as a preventive tool, during the development of hypertension.…”

Section: Discussionmentioning

confidence: 99%

“…However, until now, there have been no studies in this model evaluating the effect of dietary EWH supplementation on vascular damage. The direct vasorelaxant effect of EWH and its isolated bioactive peptides were previously described in conductance and resistance arteries [ 15 , 16 , 19 , 21 , 22 , 23 , 24 ]. Fujita et al (1995) isolated ovokinin peptide, whose amino acid sequence (Phe-Arg-Ala-Asp-His-Pro-Phe-Leu) was also identified in this EWH.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, bioactive peptides derived from food proteins with antihypertensive and antioxidant properties have been reported to be successfully used to control hypertension and related disorders. In previous works, enzymatic hydrolysis of egg white proteins with pepsin for eight hours produced multifunctional peptides with angiotensin-converting enzyme (ACE) inhibitory action and antioxidant, anti-inflammatory, and vasorelaxant activities [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. A blood- pressure-lowering effect related to ACE inhibition and antioxidant activity was also demonstrated after short- and long-term administration of this hydrolysate on a genetic model of hypertension––spontaneously hypertensive rats (SHR) [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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ROS Suppression by Egg White Hydrolysate in DOCA-Salt Rats—An Alternative Tool against Vascular Dysfunction in Severe Hypertension

et al. 2022

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This study aimed to evaluate the potential for lowering blood pressure and beneficial effects on mesenteric resistance arteries (MRA) and conductance vessels (aorta) produced by dietary supplementation of an egg white hydrolysate (EWH) in rats with severe hypertension induced by deoxycorticosterone plus salt treatment (DOCA-salt), as well as the underlying mechanisms involved. The DOCA-salt model presented higher blood pressure, which was significantly reduced by EWH. The impaired acetylcholine-induced relaxation and eNOS expression observed in MRA and aorta from DOCA-salt rats was ameliorated by EWH. This effect on vessels (MRA and aorta) was related to the antioxidant effect of EWH, since hydrolysate intake prevented the NF-κB/TNFα inflammatory pathway and NADPH oxidase-induced reactive oxygen species (ROS) generation, as well as the mitochondrial source of ROS in MRA. At the plasma level, EWH blocked the higher ROS and MDA generation by DOCA-salt treatment, without altering the antioxidant marker. In conclusion, EWH demonstrated an antihypertensive effect in a model of severe hypertension. This effect could be related to its endothelium-dependent vasodilator properties mediated by an ameliorated vessel’s redox imbalance and inflammatory state.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ROS Suppression by Egg White Hydrolysate in DOCA-Salt Rats—An Alternative Tool against Vascular Dysfunction in Severe Hypertension

et al. 2022

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Egg White Hydrolysate Mitigates Cadmium-induced Neurological Disorders and Oxidative Damage

Pinheiro Júnior,

Sosa,

das Neves

et al. 2024

Neurochem Res

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We aimed to investigate whether the consumption of Egg White Hydrolysate (EWH) acts on nervous system disorders induced by exposure to Cadmium (Cd) in rats. Male Wistar rats were divided into (a) Control (Ct): H2O by gavage for 28 days + H2O (i.p. − 15th − 28th day); (b) Cadmium (Cd): H2O by gavage + CdCl2 − 1 mg/kg/day (i.p. − 15th − 28th day); (c) EWH 14d: EWH 1 g/kg/day by gavage for 14 days + H2O (i.p.- 15th − 28th day); (d) Cd + EWH cotreatment (Cd + EWHco): CdCl2 + EWH for 14 days; (e) EWH 28d: EWH for 28 days; (f) EWHpre + Cd: EWH (1st − 28th day) + CdCl2 (15th − 28th day). At the beginning and the end of treatment, neuromotor performance (Neurological Deficit Scale); motor function (Rota-Rod test); ability to move and explore (Open Field test); thermal sensitivity (Hot Plate test); and state of anxiety (Elevated Maze test) were tested. The antioxidant status in the cerebral cortex and the striatum were biochemically analyzed. Cd induces anxiety, and neuromotor, and thermal sensitivity deficits. EWH consumption prevented anxiety, neuromotor deficits, and alterations in thermal sensitivity, avoiding neuromotor deficits both when the administration was performed before or during Cd exposure. Both modes of administration reduced the levels of reactive species, and the lipid peroxidation increased by Cd and improved the striatum’s antioxidant capacity. Pretreatment proved to be beneficial in preventing the reduction of SOD activity in the cortex. EWH could be used as a functional food with antioxidant properties capable of preventing neurological damage induced by Cd.

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Investigation of the optimal production conditions for egg white hydrolysates and physicochemical characteristics

et al. 2023

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Multi-functional egg white hydrolysate prevent hypertension and vascular dysfunction induced by cadmium in rats

Cited by 6 publications

References 73 publications

ROS Suppression by Egg White Hydrolysate in DOCA-Salt Rats—An Alternative Tool against Vascular Dysfunction in Severe Hypertension

ROS Suppression by Egg White Hydrolysate in DOCA-Salt Rats—An Alternative Tool against Vascular Dysfunction in Severe Hypertension

Egg White Hydrolysate Mitigates Cadmium-induced Neurological Disorders and Oxidative Damage

Investigation of the optimal production conditions for egg white hydrolysates and physicochemical characteristics

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