Multi-layer Cortical Ca&lt;sup&gt;2+&lt;/sup&gt; Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Gulati, Srishti; Cao, Vania Y.; Otte, Stephani

doi:10.3791/55579-v

Cited by 3 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 10-14 days from the virus injection, gradient reflective index (GRIN) lens implantation surgery was conducted as previously demonstrated 83 . Mice received subcutaneously injection of carprofen (5mg/Kg) and dexamethasone (5mg/Kg) 1 hour before the surgery.…”

Section: Surgical Procedures For In Vivo Calcium Imagingmentioning

confidence: 99%

Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisions

Choi,

Lee

2024

Preprint

View full text Add to dashboard Cite

Decision-making in mammals is fundamentally based on the integration of several senses. When different sensory inputs guide conflicting outcomes, animals make decisions following the prepotent sensory modality. However, the neural basis of state-dependent flexibility in sensory dominance and multisensory decisions remains obscure. Here, we found that locomotion switched auditory-dominant decisions to visual-dominant ones in mice with audiovisual conflicts. When mice made auditory-dominant decisions, the visual representation in the posterior parietal cortex (PPC) was weakened. Prolonged locomotion restored visual representation in the PPC and induced visually dominant decisions by inhibiting the auditory cortical neurons projecting to the PPC (ACPPC). In contrast, it had no effect on the AC projection to the striatum, which mediated auditory decisions. Locomotion activated the AC-projecting secondary motor cortex neurons, which preferentially inhibited the ACPPC neurons. Our findings demonstrate that animals make flexible multisensory decisions by gating auditory afferents to the association cortex depending on locomotor conditions.

show abstract

Section: Surgical Procedures For In Vivo Calcium Imagingmentioning

confidence: 99%

Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisions

Choi,

Lee

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Advancements in neuroscience have been significantly accelerated by optical methods that allow researchers to study neural activity and its relationship with animal behavior 1,2,3 . Microendoscopic calcium imaging with miniature microscopes is a powerful approach for studying the relationship between neural activity and behavior in freely behaving animals 4,5,6,7,8 . The technique relies on the use of genetically encoded calcium indicators, most commonly GCaMP, to monitor large-scale neural activity in specific anatomically and genetically defined cell populations 9,10 .…”

Section: Introductionmentioning

confidence: 99%

Viral vector eluting lenses for single-step targeted expression of genetically-encoded activity sensors for in vivo microendoscopic calcium imaging

Jons,

Cheng,

Dong

et al. 2023

Preprint

View full text Add to dashboard Cite

Optical methods for studying the brain are powerful tools for understanding how neural activity underlies complex behavior. These methods typically rely on genetically encoded sensors and actuators to monitor and control neural activity. For microendoscopic calcium imaging, injection of a virus followed by implantation of a lens probe is required to express a calcium sensor and enable optical access to the target brain region. This two-step process poses several challenges, chief among them being the risks associated with mistargeting and/or misalignment between virus expression zone, lens probe and target brain region. Here, we engineer an adeno-associated virus (AAV)-eluting polymer coating for gradient refractive index (GRIN) lenses enabling expression of a genetically encoded calcium indicator (GCaMP) directly within the brain region of interest upon implantation of the lens. This approach requires only one surgical step and guarantees alignment between GCaMP expression and lens in the brain. Additionally, the slow viral release from these coatings increases the working time for surgical implantation, expanding the brain regions and species amenable to this approach. These enhanced capabilities should accelerate neuroscience research utilizing optical methods and advance our understanding of the neural circuit mechanisms underlying brain function and behavior in health and disease.

show abstract

Multimodal methods to help interpret resting-state fMRI

Zhou

Jiang

Man

et al. 2023

Advances in Resting-State Functional MRI

View full text Add to dashboard Cite

Multi-layer Cortical Ca<sup>2+</sup> Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Cited by 3 publications

References 7 publications

Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisions

Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisions

Viral vector eluting lenses for single-step targeted expression of genetically-encoded activity sensors for in vivo microendoscopic calcium imaging

Multimodal methods to help interpret resting-state fMRI

Contact Info

Product

Resources

About