Multi-level metabolic engineering of Pseudomonas mutabilis ATCC31014 for efficient production of biotin

Claisen Condensation Reaction Mediated Pimelate Biosynthesis via the Reverse Adipate‐Degradation Pathway and Its Isoenzymes

“…At present, how to assemble the biotin bicyclic ring had been widely studied. Regarding the synthesis of pimeloyl thioesters, it was mainly divided into two pathways, one was the BioC‐BioH pathway in E. coli , [30] and the other was the BioI‐BioW pathway in Bacillus [8,31] . The above two pathways both require a multi‐enzyme system to carry out multi‐step catalytic reactions, which had the disadvantage of too many reaction steps and were not conducive to the follow‐up modification.…”

“…Regarding the synthesis of pimeloyl thioesters, it was mainly divided into two pathways, one was the BioC-BioH pathway in E. coli, [30] and the other was the BioI-BioW pathway in Bacillus. [8,31] The above two pathways both require a multi-enzyme system to carry out multi-step catalytic reactions, which had the disadvantage of too many reaction steps and were not conducive to the follow-up modification. Herein, we explored the biosynthetic pathway of pimelic acid, realizing the biosynthesis of pimelic acid by Claisen condensation reaction.…”

“…At the same time, some key enzymes in the pathway were easy to form inclusion bodies, so it was difficult to conduct in‐depth research on their catalytic mechanisms [8] . Meanwhile, overexpression of the above genes may disturb fatty acid synthesis and inhibit cell growth as well [8] …”

Embracing Nature’s Catalysts: A Viewpoint on the Future of Biocatalysis

“…Miscellaneous route scouting tools are available or being developed by different companies in order to design more efficient processes for certain types of molecules. A broad portfolio of mostly smaller but high value products ranging from flavor and fragrance compounds, e.g., ambrox, ,, biotin vitamins, , or pheromones , can be manufactured based on biocatalysis. In the future, emerging new and innovating products, like sweeteners, dyestuff, oligosaccharides, functional peptides, nutraceuticals, just to mention a few, will be manufactured using the technological advantage of biocatalytic routes.…”

Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum

“…Therefore, it is necessary to adopt appropriate strategies to attenuate the expression of argF. Metabolic engineering strategies, such as changing the ribosome binding site (Xiao et al, 2019), replacing the promoter (Xu et al, 2019), changing the translation initiation codon (Otten et al, 2015), and transcription interference by clustered regular interspaced short palindromic repeats (CRISPR) (Cleto et al, 2016;Yoon and Woo, 2018;Park et al, 2019) were successfully applied to optimize the flux of the metabolic pathway. The expression of argF was attenuated by changing the ribosome binding site and the translation initiation codon.…”