Multi-peak incorporation of H3-proline into oral collagen

Collagen biosynthesis, as measured by conversion of 3‐4 3H‐proline to 3H‐hydroxyproline, was studied in guinea pig oral tissue and skin during normal growth, ascorbic acid depletion and following administration of ascorbic acid to scorbutic animals. Collagen breakdown in oral and skin tissue of normal animals was determined by measuring the depletion of 14C‐hydroxyproline over a seven month period of time. Oral collagen demonstrated a high level of metabolic activity, thus providing biochemical support to the histo‐pathological concept that periodontal tissues exist in a state of continuous wound healing and repair.

Section: Discussionsupporting

confidence: 76%

Section: Tissue Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oral collagen biosynthesis in the guinea pig

Claycomb

Summers

Dvorak

1967

“…Incorporation of 'H-fucose into extracellular tissue was rapid and demonstrated several peaks (maximal grain counts: 3 h in 6-month, 6 h in 6-wk mice) which represented the net effect of synthesis and degradation of fucosecontaining giycoproteins and their precursors. Multi-peak incorporation has been reported in transseptal ligament labeled with other radioisotopes PHgalactose (Tonna & Wysor 1980), ^Hthymidine (Stahl, Tonna & Weiss 1970, Tonna, Weiss & Stahl 1972, 'H-prohne (Claycomb & Summers 1965)] and may be related to cellular circadian rhythm (Tonna & Wysor 1980). Also, turnover of cell surface glycoproteins may be more rapid that that of the extracellular matrix, possibly explaining early variations in labeling indices.…”

Section: Discussionmentioning

confidence: 97%

Distribution of ³H‐fucose in the transseptal ligament of the mouse

Johnson

1988

Johnson RB.: Distribution of^H-fucose in the transseptal ligament of the mouse. J Periodont Res 1988; 23: 363-369. The present study demonstrated 'H-fucose distribution in three regions of the transseptal hgament; the middle, mesial (adjaeent to the first molar tooth) and distal (adjacent to the second molar tooth) thirds; of 6-week-and 6-month-old Swiss mice. Incorporation of the isotope was rapid and maximal peaks occurred at 3 hours in most regions of 6-month and at 12 h in most regions of 6-wk animals: highest grain counts were over the middle third of the ligament of 6-wk animals (p< 0.001). During incorporation, mean grain counts were significantly different as a function of age (p<0.001), region (p<0.01) and time (p<0.001) and there was a significant interaction between region and time (p< 0.005). Grain removal occurred at a slower rate: there were significant differences in mean grain counts as a function of age (p<0.001), region (p<0.001) and time (p<0.00!) and a significant interaction between time and region (p<0.001) and between time, region and age (p<0.001). The highest rate of grain removal was in the middle third of the ligament of 6-wk animals. Half-hfe of labeled glycoproteins was shortest in the ligament of 6-wk animals. The study suggested regional differences in metabolism of fucose-containing glycoproteins of the transseptai ligament: highest turnover rates in the 1) middle third, and 2) young animals.

“…These investigators employed the administration of a single dose of radiotracer followed by serial killing. Tetracyclines (Harcourt et al 1962, Soni & Messer 1970, Cleall 1974, Yen & Shaw 1974, 1975a, other fluorochromes (Modis et al 1969, Sullivan 1972, and lead acetate (Yen & Shaw 1974, 1975a are currently used in multiple marking techniques. Such methods, however, do not furnish concomitant cellular data, since labeling does not occur via cellular uptake and turnover, but as a result of incorporation at mineralizing surfaces (Modis et al 1969, Sullivan 1972, Yen & Shaw 1974.…”

Section: Discussionmentioning

confidence: 99%

Application of the H³‐proline topographic method to dental tissues

Tonna

1975

Assessment is made in the present study of the applicability of the H3‐proline autoradiographic topographic method to dental and supportive parodontal tissues. Furthermore, comparisons are made of the distribution and rates of matrix production of the mineralized tissues, dentin, cementum and alveolar bone at the maxillary first molars and incisors of 5‐week‐old, short‐lived strain of BNL Swiss albino mice. The results show that tooth collagen matrix production by dentogenic and cementogenic tissues is signicantly larger than by dental or appendicular osteogenic tissues, with incisor dentin formation exceeding all surface activities evaluated to date. It is concluded that the H3‐proline topographic method is a sensitive technique offering greater refinement, and a broader spectrum of analytic possibilities than presently used technology. Integrated cellular and matrical evaluation is achieved, thus adding a new dimension to the assessment of current problems in oral biology.