Multibiofunctional TFEB-engineered endothelial progenitor cell-derived extracellular vesicles/hydrogel system for rescuing critical limb ischemia

Zhao, Chen; Zheng, Xiaohui; Wei, Xinbo; Liao, Guoyu; Yang, Depeng; Liu, Haifeng; Fan, Yubo

doi:10.1016/j.cej.2023.141730

Cited by 6 publications

(2 citation statements)

References 70 publications

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“…1 Restricted blood supply would lead to irreversible loss of functionality, which eventually leads to necrosis of limb cells and even results in amputation or death. 2 Therefore, in recent years, many investigators have tried to stimulate revascularization by promoting angiogenesis. For example, Est-Witte et al constructed end-modified poly(β-amino ester) (PBAE) nanocomplexes to induce angiogenesis by efficiently delivering HIF-1α gene to human adipose-derived stem cells (hASCs).…”

Section: Introductionmentioning

confidence: 99%

“…Lower limb ischemia is a multifactorial disease worldwide, characterized by reduced tissue blood flow due to vascular injury . Restricted blood supply would lead to irreversible loss of functionality, which eventually leads to necrosis of limb cells and even results in amputation or death . Therefore, in recent years, many investigators have tried to stimulate revascularization by promoting angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Poly(ethylenimine)-Cyclodextrin-Based Cationic Polymer Mediated HIF-1α Gene Delivery for Hindlimb Ischemia Treatment

Li,

Lu,

et al. 2024

ACS Appl. Bio Mater.

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Hindlimb ischemia is a common disease worldwide featured by the sudden decrease in limb perfusion, which usually causes a potential threat to limb viability and even amputation or death. Revascularization has been defined as the gold-standard therapy for hindlimb ischemia. Considering that vascular injury recovery requires cellular adaptation to the hypoxia, hypoxiainducible factor 1 α (HIF-1α) is a potential gene for tissue restoration and angiogenesis. In this manuscript, effective gene delivery vector PEI-β-CD (PC) was reported for the first application in the hindlimb ischemia treatment to deliver HIF-1α plasmid in vitro and in vivo. Our in vitro finding demonstrated that PC/HIF-1α-pDNA could be successfully entered into the cells and mediated efficient gene transfection with good biocompatibility. More importantly, under hypoxic conditions, PC/HIF-1α-pDNA could up-regulate the HUEVC cell viability. In addition, the mRNA levels of VEGF, Ang-1, and PDGF were upregulated, and transcriptome results also demonstrated that the cell-related function of response to hypoxia was enhanced. The therapeutic effect of PC/HIF-1α-pDNA was further estimated in a murine acute hindlimb ischemia model, which demonstrated that intramuscular injection of PC/HIF-1α-pDNA resulted in significantly increased blood perfusion and alleviation in tissue damage, such as tissue fibrosis and inflammation. The results provide a rationale that HIF-1αmediated gene therapy might be a practical strategy for the treatment of limb ischemia.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly(ethylenimine)-Cyclodextrin-Based Cationic Polymer Mediated HIF-1α Gene Delivery for Hindlimb Ischemia Treatment

Li,

Lu,

et al. 2024

ACS Appl. Bio Mater.

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Genetically Engineered Cellular Nanovesicles: Theories, Design and Perspective

Cheng,

Li,

et al. 2024

Adv Funct Materials

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The use of cellular nanovesicles (CNVs) is a groundbreaking innovation in biomedical applications. Both natural extracellular vesicles (EVs) and artificial cell membrane nanovesicles (AVs) have emerged as innovative CNVs, but they have limitations in therapeutic effects and targeting abilities. Challenges such as stability, immunogenicity, and drug payload capacity hinder their widespread application. Genetic engineering has matured as a widely employed modification strategy, leading to the development of genetically engineered extracellular vesicles (gEVs) and genetically engineered artificial cell membrane nanovesicles (gAVs). This review meticulously examines the diverse types and inherent characteristics of CNVs, alongside various surface engineering strategies for CNVs, with a specific focus on elucidating the attributes and detailing the preparation methods relevant to gEVs and gAVs. Furthermore, this exploration delves into the expansive landscape of biomedical applications, developmental prospects, and current challenges associated with the utilization of gEVs and gAVs. With a comprehensive approach, the primary objective is to provide insights that not only illuminate the nuanced intricacies of these nanovesicles but also pave the way for their seamless integration into clinical research and eventual translation into practical applications.

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Conductive hydrogel with responsive release of Herbal-Derived carbon dots for neurovascular reconstruction in diabetic critical limb ischemia

Zhou,

Zhao,

Fang

et al. 2024

Chemical Engineering Journal

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Multibiofunctional TFEB-engineered endothelial progenitor cell-derived extracellular vesicles/hydrogel system for rescuing critical limb ischemia

Cited by 6 publications

References 70 publications

Poly(ethylenimine)-Cyclodextrin-Based Cationic Polymer Mediated HIF-1α Gene Delivery for Hindlimb Ischemia Treatment

Poly(ethylenimine)-Cyclodextrin-Based Cationic Polymer Mediated HIF-1α Gene Delivery for Hindlimb Ischemia Treatment

Genetically Engineered Cellular Nanovesicles: Theories, Design and Perspective

Conductive hydrogel with responsive release of Herbal-Derived carbon dots for neurovascular reconstruction in diabetic critical limb ischemia

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