Multicolored silver nanoparticles for multiplexed disease diagnostics: distinguishing dengue, yellow fever, and Ebola viruses

Abstract

Rapid point-of-care (POC) diagnostic devices are needed for field-forward screening of severe acute systemic febrile illnesses. Multiplexed rapid lateral flow diagnostics have the potential to distinguish among multiple pathogens, thereby facilitating diagnosis and improving patient care. Here, we present a platform for multiplexed pathogen detection using multi-colored silver nanoplates. This design requires no external excitation source and permits multiplexed analysis in a single channel, facilitating integration and manufacturing.

Application of nanodiagnostics in point-of-care tests for infectious diseases

“…In this review, the most important nanodiagnostics-based strategies and technique platforms proposed to develop novel POC diagnosis for the detection of pathogenic agents for infectious diseases, including diagnostic magnetic resonance (DMR) platform, 71,72 magnetic barcode assay systems, 73,74 cell phone-based polarized light microscopy platform, 75,76 cell phone dongle platform, 15,77 and paper-based POCT platform are introduced and discussed. 78,79 These novel nanotechnology-based diagnostic platforms, with the advantages and drawbacks according to the ASSURED criteria listed in Table 1, are currently the most promising POC diagnosis for various infectious diseases.…”

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

“…This effort is testified also by the number of papers reporting new materials to be used as labels [10][11][12][39][40], sometimes in combination with innovative detection technologies [13,41], and of enhancement strategies aimed at increasing the sensitivity of the ICST compared to using traditional gold nanoparticles [42][43], as also summarized in two comprehensive reviews [44][45]. Within the number of novel materials for labeling antibodies in ICTS, the request for analytical devices with multiplexing capability is prompting towards using labels easily distinguishable from each other, such as nanoparticles with different colors [19][20]23]. Gold nanoparticles are widely employed as labels for ICST, thanks to well-recognized advantages, such as easy preparation, stability, easy conjugation to antibodies, brilliant and tunable color [6][7][8]45].…”

“…Metal nanoparticles also offer the opportunity of tailoring the SPR band (and thus the color) by varying NP dimension and shape [21][22]. In fact, the advantage of using differently shaped and therefore differently colored silver nanoparticles as labels for multiplex ICST has been demonstrated by Yen et al [23]. The authors reported an ICST for the simultaneous detection of three viruses, which was easily interpreted by the naked-eye through color-encoding and without needing any supplementary equipment.…”

Nanomaterial-functionalized Cellulose: Design, Characterization and Analytical Applications

“…Utilizing Ag nanoplates, Yen et al have designed a paper assay which can detect biomarkers for dengue fever, yellow fever, and the ebola virus. 99 Ortega et al also designed an assay targeting dengue fever utilizing magnetic NPs. 100 Using a similar approach, Abe et al demonstrated immunoglobulin (IgG) detection down to a concentration of 10 μg/L.…”