Multidrug resistant and ESBL-producing Salmonella spp. isolated from poultry

Abstract

Salmonella spp. is one of the main agents responsible for foodborne infection in humans, and products of poultry origin are the most common infection sources. Studies have shown the occurrence of antimicrobials resistant Salmonella spp. in animal products. The Extended Spectrum ?-Lactamase (ESBL) are enzymes that confer to bacteria the ability to hydrolyze cephalosporin with an oximino side chain and monobactams. This study aimed to investigate antimicrobial resistance profile, identify phenotypes and genotypes for multiple drug resistance (MDR) and that produce ESBL from isolates of Salmonella spp. in the broiler production chain. We used samples of Salmonella spp. (n=11) isolates from poultry, poultry products and poultry-source environment from the state of Maranhão - Brazil. The isolates of Salmonella spp. assessed showed genotypical and phenotypical characteristics of MDR. The results show that 72.72% (08/11) of the strains presented the phenotypic profile for ESBL production. The isolates showed positivity to at least 13.64% (03/22) of the genes studied and the highest frequencies were observed in genes sul1 (73%), dfrA12 (55%), blaCTX-M (55%), tetA, tetB and tetC, with 45%. In conclusion, the strains of Salmonella spp. isolates present genotypic and phenotypic characteristics for MDR and ESBL production, demonstrating the dissemination risk of these microorganisms through the food chain.

Estimation of the Prevalence of Antimicrobial Resistance in Badgers (Meles meles) and Foxes (Vulpes vulpes) in Northern Ireland

“…ESBLs Salmonella spp. have been detected in chickens ( Dierikx et al, 2010 ; de Souza et al, 2019 ) and other livestock ( Riano et al, 2006 ). ESBL Salmonella isolations from wildlife are uncommon but ESBL S. Infantis isolated from owls have been reported ( Fuentes-Castillo et al, 2019 ).…”

Plasmid-Mediated Antibiotic Resistance and Class 1 Integron in Salmonella Heidelberg Isolated from Poultry Farms in Santander - Colombia

“…The antibiotic resistance genotypes as bla CMY2 have been found in poultry farms in Brazil (Biffi et al, 2014) and in Colombia, where 168 isolates from different poultry production levels (farms, slaughter, and retail) were positive to bla CMY2 gene (Castellanos et al, 2018). The presence of Extended Spectrum β-lactamase (ESBL) codified by bla CMY2 and other genes from this group reduce the effectiveness of antibiotics such as ceftriaxone, ceftazidime, and cefotaxime by the hydrolyzing of the β-lactam ring preventing the union with the penicillin-binding protein (PBP) (De Souza et al, 2019;Kong et al, 2010).…”

“…These genes were found in poultry farms from Egypt with values of 97.3% and 5.3%, respectively (Abdel-Maksoud et al, 2015) and sul2 gene in isolates from chickens sold at retail in Colombia (57.4%) (Cortés et al, 2017). The tetB gene was found in broiler chickens from Portugal (45.4%) (De Souza et al, 2019) and in chicken carcasses retailed in Colombia (42.5%) (Cortés et al, 2017). This gene codes for a tetracycline resistance protein, which promotes the elimination of tetracycline from the inside of cells by an energy-dependent efflux pump (Grossman, 2016).…”

A Importância Da Nutrição Na Seletividade Alimentar De Crianças Autistas

“…Os isolados de Salmonella oriundos de produtos de origem avícola são frequentemente considerados fenótipos com resistência múltipla a drogas (BRITO et al, 2020;SOUZA et al, 2019a). Essa realidade é considerada um problema mundial de saúde pública, visto que há possibilidades de genes de resistência dessas bactérias de origem alimentar serem veiculados e incorporados à microbiota humana, reduzindo a eficácia do tratamento com antibióticos (MAIA, 2020).…”