Multilineage Differentiation of Rhesus Monkey Embryonic Stem Cells in Three‐Dimensional Culture Systems

Chen, Silvia S.; Revoltella, Roberto P.; Papini, Sandra; Michelini, Monica; Fitzgerald, Wendy; Zimmerberg, Joshua; Margolis, Leonid

doi:10.1634/stemcells.21-3-281

Cited by 83 publications

(47 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In order to solve the problems, 3D model arises at this historic moment. In recent years, many experiments were conducted to study the maintenance and differentiation of stem cells by 3D such as Embryonic Stem Cells are much easier differentiated into epithelial cells in collagen 3D culture [69] . In 3D culture, prostate progenitor cells (NHPrE1) with characteristics of stem cells appeared to be as dendritic growth and prostate epithelial stromal cells (BHPrE1) were not, suggesting that the former contains a higher proportion of stem cells / progenitor cells [70] .…”

Section: A Three Dimension (3d) In Vitro Modelmentioning

confidence: 99%

Progress in isolation and enrichment of cancer stem cells

Zhu

Sun²

2017

Can Cell Microenviron

View full text Add to dashboard Cite

Cancer stem cells (CSC) are a group of cells with capacity of self renewal and can proliferate into a heterogeneous bulk with cancer progeny population. This is the exactly primary reason for metastasis and recurrence of tumors. Strategies for studying cancer's targeted therapy and biological property mainly include its isolation and enrichment. In the coming era it will also be the focus of significant value. We will summarize the current strategies used for isolation and enrichment of CSC, including serum-free suspension culture, feeder cell layers, immunoselection based on cell surface markers, side population cells, resistance to radiation treatment and chemotherapy, combined utilization of various strategies and a three dimension (3D) in vitro model in this review.

show abstract

Section: A Three Dimension (3d) In Vitro Modelmentioning

confidence: 99%

Progress in isolation and enrichment of cancer stem cells

Zhu

Sun²

2017

Can Cell Microenviron

View full text Add to dashboard Cite

show abstract

“…[89]) or for grafting procedures [13]. So far, other epithelial tissues have been successfully modelled in 3-D ECM cultures (see, for example [34,95]), and heterologous 3-D organotypic cultures have been extensively used in order to study interactions between different cell types in normal and pathological conditions ( [45,67,68] and references therein), to simulate in vitro human malignant tumours [25,81], to investigate factors that regulate stem cell fate [17], and to learn about virus-host interactions [2]. Table 1 lists the principal 3-D cell culture systems currently employed in basic and applied research.…”

Section: Toward Tissue-like Cell Systemsmentioning

confidence: 99%

Modelling tissues in 3D: the next future of pharmaco-toxicology and food research?

2008

View full text Add to dashboard Cite

The development and validation of reliable in vitro methods alternative to conventional in vivo studies in experimental animals is a well-recognised priority in the fields of pharmaco-toxicology and food research. Conventional studies based on two-dimensional (2-D) cell monolayers have demonstrated their significant limitations: the chemically and spatially defined three-dimensional (3-D) network of extracellular matrix components, cell-to-cell and cell-to-matrix interactions that governs differentiation, proliferation and function of cells in vivo is, in fact, lost under the simplified 2-D condition. Being able to reproduce specific tissue-like structures and to mimic functions and responses of real tissues in a way that is more physiologically relevant than what can be achieved through traditional 2-D cell monolayers, 3-D cell culture represents a potential bridge to cover the gap between animal models and human studies. This article addresses the significance and the potential of 3-D in vitro systems to improve the predictive value of cell-based assays for safety and risk assessment studies and for new drugs development and testing. The crucial role of tissue engineering and of the new microscale technologies for improving and optimising these models, as well as the necessity of developing new protocols and analytical methods for their full exploitation, will be also discussed.

show abstract

“…In vitro differentiation into numerous lineages, including neural (Zhang et al, 2001;Rathjen et al, 2002;Wichterle et al, 2002;Nakayama et al, 2003), hematopoietic (Kaufman et al, 2001), osteo/chondrogenic (Buttery et al, 2001;Hegert et al, 2002;Sottile et al, 2003;Sui et al, 2003), myogenic (Muller et al, 2000;Kehat et al, 2001;Mummery et al, 2003;Zandstra et al, 2003), and endothelial (Yamashita et al, 2000;Kaufman et al, 2004) has been reported. For organ and tissue engineering, the intersection between directeddifferentiation by chemical means, such as growth factors and cytokines (Schuldiner et al, 2000), and innovative 3-dimensional culture techniques within biodegradable scaffolds and matrices (Kim and Mooney, 1998;Richardson et al, 2001;Chen et al, 2003;Levenberg et al, 2003;Liu et al, 2003) is likely to be particularly fruitful.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%