Multilineage differentiation potential of human dermal skin‐derived fibroblasts

Abstract

Dermal skin-derived fibroblasts from rodent and human have been found to exhibit mesenchymal surface antigen immunophenotype and differentiation potential along the three main mesenchymal-derived tissues: bone, cartilage and fat. Human dermal skin-derived mesenchymal stem cells constitute a promising cell source in clinical applications. Therefore, we isolated fibroblastic mesenchymal stem-cell-like cells from human dermis derived from juvenile foreskins, which share a mesenchymal stem cell phenotype and multi-lineage differentiation potential. We could show similar expression patterns for CD14()), CD29(+), CD31()), CD34()), CD44(+), CD45()), CD71(+), CD73 ⁄ SH3-SH4(+), CD90 ⁄ Thy-1(+), CD105 ⁄ SH2(+), CD133()) and CD166 ⁄ ALCAM(+) in wellestablished adipose tissue derived-stem cells and fibroblastic mesenchymal stem-cell-like cells by flow cytometry. Immunostainings showed that fibroblastic mesenchymal stem-celllike cells expressed vimentin, fibronectin and collagen; they were less positive for a-smooth muscle actin and nestin, while they were negative for epithelial cytokeratins. When cultured under appropriate inducible conditions, both cell types could differentiate along the adipogenic and osteogenic lineages. Additionally, fibroblastic mesenchymal stem-cell-like cells demonstrated a high proliferation potential. These findings are of particular importance, because skin or adipose tissues are easily accessible for autologous cell transplantations in regenerative medicine. In summary, these data indicate that dermal fibroblasts with multilineage differentiation potential are present in human dermis and they might play a key role in cutaneous wound healing.

Surface markers distinguishing mesenchymal stem cells from fibroblasts

“…It was shown that human bone marrow MSC (BM-MSC) surface markers CD9, CD29, CD44, CD73, CD90, CD105, CD166 were also expressed on human dermal fibroblasts (10). Lorenz K and colleagues (11) showed similar expression patterns for CD14(-), CD29(+), CD31(-), CD34(-), CD44(+), CD45(-), CD71(+), CD73(+), CD90(+), CD105(+), CD133(-) and CD166(+) in human adipose tissue-derived stem cells and human dermal skin-derived fibroblasts. Cappellesso-Fleury S and colleagues (4) compared the expression of 25 surface markers of human BM-MSCs and human dermal fibroblasts.…”

Derivation of Functional Smooth Muscle Cells from Multipotent Human Hair Follicle Mesenchymal Stem Cells

“…[21][22][23][24][25] Comparison between fibroblasts from interfollicular skin and hair follicles prompted some investigators to suggest that the niche of skin multipotent stem cells resides in the hair follicle. 7,26,27 Indeed, two recent studies confirmed the multipotent nature of rat hair follicle cells and showed similar differentiation potential to BM-MSCs.…”

Hair Follicle Reconstruction and Stem Cells

“…At the same time, DP cells meet stem cell criteria. In a number of works, including those coming from our lab, it was demonstrated that DP cells, according to their characteristics, may be atributed to mesenchymal stem cells along with those derived from bone marrow and adipose tissue [23][24][25][26]. These characteristics are widely accepted criteria for mesenchymal stem cells including ibroblastic morphology, ability to adhere and to diferentiate into osteogenic, chondrogenic, and adipogenic lineages [24,27,28].…”