Multilocus sequence typing-based analysis of Moraxella catarrhalis population structure reveals clonal spreading of drug-resistant strains isolated from childhood pneumonia

Du, Yijun; Zhou, Haijian; Wang, Fei; Liang, Shengnan; Cheng, Lihong; Du, Xiaofei; Pang, Feng; Tian, Jinjing; Zhao, Jinxing; Kan, Biao; Xu, Jianguo; Li, Juan; Zhang, Furong

doi:10.1016/j.meegid.2017.11.018

Cited by 10 publications

(13 citation statements)

References 31 publications

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“…Nevertheless, all M. catarrhalis isolates in our study were susceptible to imipenem. This was consistent with findings in China 7 . This finding suggests that this antibiotic may be useful in treating M. catarrhalis infection in children with pneumonia.…”

Section: Discussionsupporting

confidence: 92%

“…catarrhalis was the only bacterial isolate, which was comparable to a finding in China. 7 This was supported by the detection of predominantly Gram-negative diplococci in the sputum and bacterial cultures, which were later confirmed to be M. catarrhalis by PCR and DNA sequencing. These findings were supported by a previous study in Belgium which showed that the presence of M. catarrhalis in sputum was rarely due to oropharyngeal or nasopharyngeal contamination, as evidenced by the high isolation rate of this organism from good quality samples.…”

Section: Discussionmentioning

confidence: 83%

“…M. catarrhalis resistance to commonly used antimicrobials has risen, particularly to β‐lactam antibiotics, 5 and resistance is driven by two types of BRO enzymes: BRO‐1 and BRO‐2, encoded by the bro‐1 and bro‐2 genes 6 . In addition, recent reports indicate an increase in multi‐drug resistant strains 7,8 . This suggests that new antibiotics or combinations may be required for the treatment of M. catarrhalis infections in the future.…”

Section: Introductionmentioning

confidence: 99%

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Microbiologic and virulence characteristics ofMoraxella catarrhalisisolates from Zambian children presenting with acute pneumonia

Nawa

Mwansa

Mwaba

et al. 2022

Pediatric Pulmonology

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Background: Moraxella catarrhalis is one of the bacterial pathogens associated with childhood pneumonia, but its clinical importance is not clearly defined. Objective: This study aimed to investigate the microbiologic and virulence characteristics of M. catarrhalis isolates obtained from children with pneumonia in Lusaka, Zambia. Methods: This retrospective, cross-sectional study analyzed 91 M. catarrhalis isolates from induced sputum samples of children less than 5 years of age with pneumonia enrolled in the Pneumonia Etiology Research for Child Health study in Lusaka, Zambia between 2011 and 2014. Bacteria identification and virulence genes detection were performed by PCR and DNA sequencing, while antimicrobial susceptibility testing was determined by the Kirby-Bauer method.Results: All the M. catarrhalis isolates were obtained from good-quality sputum samples and were the predominant bacteria. These isolates harbored virulence genes copB (100%), ompE (69.2%), ompCD (71.4%), uspA1 (92.3%), and uspA2 (69.2%) and were all β-lactamase producers. They showed resistance to ampicillin (100%), amoxicillin (100%), trimethoprim-sulfamethoxazole (92.3%), ciprofloxacin (46.2%), chloramphenicol (45.1%), erythromycin (36.3%), tetracycline (25.3%), cefuroxime (11.0%), and amoxicillin-clavulanate (2.2%), with 71.4% displaying multi-drug resistant phenotype but all susceptible to imipenem (100%). Conclusion:This study showed that M. catarrhalis isolates were the predominant or only bacterial isolates from the sputum samples analyzed. The findings provide supportive evidence for the pathogenic potential role of this bacterium in pediatric

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

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Microbiologic and virulence characteristics ofMoraxella catarrhalisisolates from Zambian children presenting with acute pneumonia

Nawa

Mwansa

Mwaba

et al. 2022

Pediatric Pulmonology

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“…The fitness cost of antibiotic resistance is a phenomenon in which antibiotic-resistant mutants exhibit decreased fitness and virulence without an apparent reason [ 7 , 8 ]. Previously, we had demonstrated that macrolide-resistant isolates were highly concentrated in the CC449 (CCN10) and CC363 clonal complexes [ 9 , 10 ], while macrolide-susceptible isolates were highly concentrated in the CC224 and CC446 (CCN08) clonal complexes [ 9 ]. We hypothesized that populations of M. catarrhalis isolates with different macrolide susceptibilities exhibit differential pathogenic behaviours, such as the ability to adhere and invade human epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells

Liu

Ding

Jia

et al. 2022

Emerging Microbes & Infections

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Recently, the prevalence of macrolide-resistant Moraxella catarrhalis has been reported, especially among Chinese children. The fitness cost of resistance is reported to render the resistant bacteria less virulent. To investigate the correlation between macrolide susceptibility of M. catarrhalis and pathogenicity, the whole genome of 70 M. catarrhalis isolates belonging to four clonal complexes with different macrolide susceptibilities was sequenced. The gene products were annotated with the Gene Ontology terms. Based on 46 extracted essential virulence genes, 19 representative isolates were selected to infect type II alveolar cells (A549 cells). The ability of these isolates to adhere and invade human epithelial cells and to produce cytokines was comparatively analysed. Furthermore, mice were infected with a pair of M. catarrhalis isolates with different pathogenic behaviours and macrolide susceptibilities to examine pulmonary clearance, histological findings, and the production of cytokines. The percentages of annotations for binding, metabolic process, cellular process, and cell were non-significantly different between the macrolide-resistant and macrolide-susceptible groups. The presence of uspA2 , uspA2H , pilO , lbpB , lex1 , modM , mboIA , and mboIB significantly differed among the four clonal complexes and macrolide susceptibility groups. Furthermore, compared with those in macrolide-susceptible isolates, the adhesion ability was stronger ( P = 0.0019) and the invasion ability was weaker ( P < 0.0001) in the macrolide-resistant isolates. Mouse experiments revealed that pulmonary macrophages elicit immune responses against M. catarrhalis infection by significantly upregulating the Csf2, Il4, Il13, Il1b, Il6, Tnf, and Il18. Therefore, M. catarrhalis populations exhibited diverse pathogenicity in vitro and in vivo .

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“…The identity of an isolate as M. catarrhalis was confirmed by colony morphology and the presence of butyrate esterase. All isolates were subjected to molecular typing by pulsed field gel electrophoresis; multilocus sequence types were determined on selected isolates [15]. Strains that were isolated at a single monthly clinic visit and were not isolated again at subsequent monthly clinic visits were classified as "cleared".…”

Section: Bacterial Strainsmentioning

confidence: 99%

Persistence of Moraxella catarrhalis in Chronic Obstructive Pulmonary Disease and Regulation of the Hag/MID Adhesin

Murphy

Brauer

Pettigrew

et al. 2018

The Journal of Infectious Diseases

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Background. Persistence of bacterial pathogens in the airways has profound consequences on the course and pathogenesis of chronic obstructive pulmonary disease (COPD). Patients with COPD continuously acquire and clear strains of Moraxella catarrhalis, a major pathogen in COPD. Some strains are cleared quickly and some persist for months to years. The mechanism of the variability in duration of persistence is unknown. Methods. Guided by genome sequences of selected strains, we studied the expression of Hag/MID, hag/mid gene sequences, adherence to human cells, and autoaggregation in longitudinally collected strains of M. catarrhalis from adults with COPD. Results. Twenty-eight of 30 cleared strains of M. catarrhalis expressed Hag/MID whereas 17 of 30 persistent strains expressed Hag/MID upon acquisition by patients. All persistent strains ceased expression of Hag/MID during persistence. Expression of Hag/ MID in human airways was regulated by slipped-strand mispairing. Virulence-associated phenotypes (adherence to human respiratory epithelial cells and autoaggregation) paralleled Hag/MID expression in airway isolates. Conclusions. Most strains of M. catarrhalis express Hag/MID upon acquisition by adults with COPD and all persistent strains shut off expression during persistence. These observations suggest that Hag/MID is important for initial colonization by M. catarrhalis and that cessation of expression facilitates persistence in COPD airways.

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Multilocus sequence typing-based analysis of Moraxella catarrhalis population structure reveals clonal spreading of drug-resistant strains isolated from childhood pneumonia

Cited by 10 publications

References 31 publications

Microbiologic and virulence characteristics ofMoraxella catarrhalisisolates from Zambian children presenting with acute pneumonia

Microbiologic and virulence characteristics ofMoraxella catarrhalisisolates from Zambian children presenting with acute pneumonia

Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells

Persistence of Moraxella catarrhalis in Chronic Obstructive Pulmonary Disease and Regulation of the Hag/MID Adhesin

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