Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy

Kukk, Anatoly Fedorov; Wu, D. S.; Gaffal, Evelyn; Panzer, Rüdiger; Emmert, Steffen; Roth, Bernhard

doi:10.1002/jbio.202200129

Cited by 14 publications

(9 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is the next development stage of the OCT-RS-US probe that was previously demonstrated in skin cancer screenings. 23 The main part of the scanning unit is built on a customizable probe head (OCTP-900, Thorlabs, USA) which was modified to include a coaxial RS collection system. The OCT signal (red) is delivered from the base unit (GAN621, Thorlabs, USA; central wavelength at 900 µm) to the setup via fiber, where it is split into reference and sample arm.…”

Section: Methodsmentioning

confidence: 99%

Multimodal imaging system with ultrasound, photoacoustics, and optical coherence tomography for optical biopsy of melanoma

Kukk

Wu²,

Gaffal³

et al. 2023

Multimodal Biomedical Imaging XVIII

View full text Add to dashboard Cite

The rate of skin cancer incidence including melanoma has been steadily increasing in the last decades. While melanoma often show little to no symptoms in the early stages, they can spread to the lymph nodes and drastically reduce survival chances in the later stages. The current gold standard for diagnosis is visual examination, excision, and histological examination of the sample tissue, which is an invasive, costly and time-consuming process. As an alternative to this procedure, we introduce a novel multimodal optical system that integrates ultrasound (US), photoacoustic tomography (PAT), and optical coherence tomography (OCT) with Raman spectroscopy (RS). The setup allows quick and non-invasive skin lesion diagnosis and the determination of 3D lesion depth, helping the dermatologists make a decision on the excision margins. The OCT delivers structural and depth information of thin skin lesions, while the US and PAT measure the penetration depth of thicker lesions and the RS analyzes the chemical composition that can be used to distinguish between benign and malignant skin lesions. In our setup, the RS and OCT share the optical path and the scanning elements, which allows colocalized measurements. The US and PAT are integrated with an acoustical reflector, which enables B-mode measurements at the same position as OCT and RS without switching the scanning head. We demonstrate the imaging capabilities of the multimodal setup on custom made agar phantoms and present first measurements on ex vivo mouse and in vivo human skin samples. We compare the results with the corresponding histological images.

show abstract

Section: Methodsmentioning

confidence: 99%

Multimodal imaging system with ultrasound, photoacoustics, and optical coherence tomography for optical biopsy of melanoma

Kukk

Wu²,

Gaffal³

et al. 2023

Multimodal Biomedical Imaging XVIII

View full text Add to dashboard Cite

show abstract

“…Among noninvasive modalities, OCT is most useful for margin and depth detection [15,16]. As a welldeveloped optical imaging technology, it can provide images of the morphology of living tissues with micrometer-level resolution, making it a very attractive technology in the medical field [17].…”

Section: Introductionmentioning

confidence: 99%

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Kukk

Roth

2023

Journal of Biophotonics

View full text Add to dashboard Cite

Melanoma is responsible for more than half of the deaths related to skin cancer in the last few decades. A dual‐modality optical biopsy system with Raman spectroscopy and optical coherence tomography approach was built with the goal of achieving noninvasive skin measurement. To mimic melanoma and evaluate the effect of melanin on skin, models have been created by dissolving synthetic melanin in dimethyl sulfoxide and adding it to fresh skin samples. Compared to the untreated samples, morphological images showed that the imaging depth on melanin‐treated skin has been increased from 250 μm to 350 μm due to the optical clearing effect of the DMSO solvent, and Raman analysis revealed that relative spectral intensities of melanin‐treated samples were lower in the amide‐I and CH2‐deformation bands, and higher in the CH2‐twist and C–C stretch bands. Using machine learning for skin type classification, an accuracy of 89% is achieved.

show abstract

“…Some latest solutions advocate for the combination of OCT with other techniques. For example, a combination with ultrasound (US) provides an overall visualization of more extensive lesions (sacrificing image resolution) and complementary molecular information can be provided by Raman spectroscopy [ 13 ]; however, all of these multimodal approaches are still in the initial steps of development. Unfortunately, none of these solutions have overcome the mentioned problems during diagnosis.…”

Section: Introductionmentioning

confidence: 99%

“…They reported differences in the attenuation factor of the epidermis when comparing malignant melanoma, dysplastic nevus, and benign nevus. The OPE (optical properties extraction) algorithm has been presented in [ 13 ] and continues to evolve in [ 19 ], where tissue-scattering coefficient, absorption coefficient, and anisotropy factor optical properties were calculated from OCT images and integrated, and then signatures associated to melanoma were identified in a trained machine learning approach. Additionally, they proposed an “optical radiomic melanoma detection (ORMD)” protocol that is aimed to be used by clinicians during diagnosis, providing hints about the tissue similarity with melanoma or a healthy status.…”

Section: Introductionmentioning

confidence: 99%

Melanoma and Nevi Subtype Histopathological Characterization with Optical Coherence Tomography

Saratxaga

Asumendi

Gardeazábal

et al. 2023

Life

View full text Add to dashboard Cite

Background: Melanoma incidence has continued to rise in the latest decades, and the forecast is not optimistic. Non-invasive diagnostic imaging techniques such as optical coherence tomography (OCT) are largely studied; however, there is still no agreement on its use for the diagnosis of melanoma. For dermatologists, the differentiation of non-invasive (junctional nevus, compound nevus, intradermal nevus, and melanoma in-situ) versus invasive (superficial spreading melanoma and nodular melanoma) lesions is the key issue in their daily routine. Methods: This work performs a comparative analysis of OCT images using haematoxylin–eosin (HE) and anatomopathological features identified by a pathologist. Then, optical and textural properties are extracted from OCT images with the aim to identify subtle features that could potentially maximize the usefulness of the imaging technique in the identification of the lesion’s potential invasiveness. Results: Preliminary features reveal differences discriminating melanoma in-situ from superficial spreading melanoma and also between melanoma and nevus subtypes that pose a promising baseline for further research. Conclusions: Answering the final goal of diagnosing non-invasive versus invasive lesions with OCT does not seem feasible in the short term, but the obtained results demonstrate a step forward to achieve this.

show abstract

Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy

Cited by 14 publications

References 45 publications

Multimodal imaging system with ultrasound, photoacoustics, and optical coherence tomography for optical biopsy of melanoma

Multimodal imaging system with ultrasound, photoacoustics, and optical coherence tomography for optical biopsy of melanoma

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Melanoma and Nevi Subtype Histopathological Characterization with Optical Coherence Tomography

Contact Info

Product

Resources

About