Multiphase Intrafibrillar Mineralization of Collagen

L, Niu; Jiao, Kai; Ryou, Heonjune; Yiu, Cky; Chen, Jihua; Breschi, Lorenzo; Arola, D.; Pashley, David H.; Tay, Franklin R.

doi:10.1002/ange.201210259

Cited by 7 publications

(8 citation statements)

References 29 publications

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“…After another 8 weeks, fibrils were closely packed, displaying a plywood-like interlaced pattern of fibril bundles, similar to the hierarchical organization found (Fig. 6F, bottom) (25,(33)(34)(35)(36). These characterization studies demonstrate that BAM scaffolds elicit the production of a mineralized matrix, with clear differences in terms of bone volume, mineral density, collagen content, and degree of mineralization compared to that observed with PLA.…”

Section: In Vitro Osteogenesis Enhanced By Bamsupporting

confidence: 77%

Bioenergetic-active materials enhance tissue regeneration by modulating cellular metabolic state

Liu

St-Pierre

et al. 2020

Sci. Adv.

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Cellular bioenergetics (CBE) plays a critical role in tissue regeneration. Physiologically, an enhanced metabolic state facilitates anabolic biosynthesis and mitosis to accelerate regeneration. However, the development of approaches to reprogram CBE, toward the treatment of substantial tissue injuries, has been limited thus far. Here, we show that induced repair in a rabbit model of weight-bearing bone defects is greatly enhanced using a bioenergetic-active material (BAM) scaffold compared to commercialized poly(lactic acid) and calcium phosphate ceramic scaffolds. This material was composed of energy-active units that can be released in a sustained degradation-mediated fashion once implanted. By establishing an intramitochondrial metabolic bypass, the internalized energy-active units significantly elevate mitochondrial membrane potential (ΔΨm) to supply increased bioenergetic levels and accelerate bone formation. The ready-to-use material developed here represents a highly efficient and easy-to-implement therapeutic approach toward tissue regeneration, with promise for bench-to-bedside translation.

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Section: In Vitro Osteogenesis Enhanced By Bamsupporting

confidence: 77%

Bioenergetic-active materials enhance tissue regeneration by modulating cellular metabolic state

Liu

St-Pierre

et al. 2020

Sci. Adv.

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“…In this way the CaP phases are deposited over the original amorphous silica phase. 24 Sodium tripolyphosphate (TPP) a linear tripolyphosphate, that has a similar structure to that of OSA, was demonstrated to induce hierarchal mineralization within selfassembling collagen brils. TPP forms ionic cross-links (ionic bridges) within collagen molecules leading to the deposition of mineral within the brils.…”

Section: Resultsmentioning

confidence: 99%

Soluble silicon patterns and templates: calcium phosphate nanocrystal deposition in collagen type 1

et al. 2016

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“…23−25 Other ex vivo studies have shown that with the presence of charged polymers such as poly(allylamine hydrochloride) (PAH), 26 poly(acrylic acid) (PAA), 27 or polyaspartic acid (PASP), 23,28 ACP clusters can be stabilized and facilitate the formation of oriented intrafibrillar HAP nanoplatelets. 29,30 Inspired by these findings, we suggest that a hydrogel of charged polymers loaded with a high concentration of ACP nanoclusters could be used as a precursor for bone regeneration via a biomineralization-like pathway. (CaCl 2 ), disodium hydrogen phosphate (Na 2 HPO 4 ), sodium hydroxide (NaOH), and PAA (Mw = 450 000 Da) were purchased from Sigma-Aldrich (Beijing, China).…”

Section: Introductionmentioning

confidence: 94%

Calcium Phosphate Nanocluster-Loaded Injectable Hydrogel for Bone Regeneration

Yao

Xu²,

Zhou

et al. 2019

ACS Appl. Bio Mater.

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Bone is a hierarchical tissue in which the extracellular matrix consists of hydroxyapatite (HAP) crystals embedded in the collagen matrix. Artificial bone regeneration remains a great challenge due to the difficulty of balancing the chemical composition, biological compatibility and mechanical performance of the implant. Biomineralization starts from the formation of a hydrogellike bio-macromolecule matrix in many cases, while the mineralization of HAP often builds from amorphous calcium phosphate (ACP) nanoclusters. Inspired by these discoveries, here we use a hydrogel loaded with ~1 nm sized polymer-stabilized ACP nanoclusters (cluster-loaded hydrogel) as an injectable bone regeneration material. The hydrogel is biocompatible and stabilizes the ACP clusters such that they could efficiently infiltrate into collagen fibrils leading to intrafibrillar mineralization of HAP nanocrystals. The ex vivo results reveal that the cluster-loaded hydrogel has an excellent bone affinity as well as provide a suitable environment for the proliferation and differentiation of bone cells. In vivo experiments with rat bone show that the cluster-loaded hydrogel can generate HAP-based fillings within bone defects with perfect bonding to the surrounding tissue and a mechanical performance comparable with native bone. The fluidity of the hydrogel is further beneficial by providing a feasible minimally invasive bone healing procedure via syringe injection. The discovery and utilization of the cluster-loaded hydrogel described here provides a promising bio-inspired approach for bone tissue regeneration.

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Multiphase Intrafibrillar Mineralization of Collagen

Cited by 7 publications

References 29 publications

Bioenergetic-active materials enhance tissue regeneration by modulating cellular metabolic state

Bioenergetic-active materials enhance tissue regeneration by modulating cellular metabolic state

Soluble silicon patterns and templates: calcium phosphate nanocrystal deposition in collagen type 1

Calcium Phosphate Nanocluster-Loaded Injectable Hydrogel for Bone Regeneration

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