Multiphoton imaging and Raman spectroscopy of the bovine vertebral endplate

Abstract

Multiphoton imaging and Raman spectroscopy reveal chemistry and micro-structure of the bovine vertebral endplate.

Spectral Features Differentiate Aging-Induced Changes in Parchment—A Combined Approach of UV/VIS, µ-ATR/FTIR and µ-Raman Spectroscopy with Multivariate Data Analysis

“…The deconvolution of the amide III bands in the Raman spectra mainly confirms the previously mentioned changes in collagen conformation ( Figure S11 ). Bands at 1340 and 1380 cm −1 correspond to CH 2 -stretchinng vibrations associated with proteoglycans and glycosaminoglycans connected with collagen via crosslinks [ 44 ]. An abrupt increase (12.38 to 15.06%) is observed after a light exposure of 250 h, while reduced band area ratios of these glycans are observed for all RH levels, particularly pronounced at 9.73% after exposure to 80%RH.…”

Characterization of the mechanical properties of the mouse Achilles tendon enthesis by microindentation. Effects of unloading and subsequent reloading

“…3 ): the ν 1 PO 4 3− peak at 960 cm −1 for phosphate apatite, the ν(C—C) phenylalanine peak at 1003 cm −1 for collagen, and the ν 1 CO 3 2− peak at 1070 cm −1 for carbonate apatite ( Das Gupta et al, 2020 ; Mandair and Morris, 2015 ). From the maximum peak intensities, two intensity ratios were determined: the mineral-to-matrix ratio, that between the phosphate peak and the phenylalanine peak, and the carbonate-to-phosphate ratio, which indicates the proportion of carbonate-substituted phosphate apatites ( Crawford-Manning et al, 2021 ; Deymier et al, 2020 ; Schwartz et al, 2013 ; Mandair and Morris, 2015 ).

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Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer

“…In fact, as suggest by Shin et al, the level of collagen is higher in broadenoma rather than invasive ductal carcinoma, which both have similar carbonate level. 49 Moreover, two photon uorescence (TPF), widely used to identify mineral tissues, 50,51 could help by identifying the BMCs in the breast tissue sections. Finally, stimulated Raman scattering (SRS), a non-linear optical technique, offers the ability to obtain complementary information to Raman spectroscopy as it allows imaging of biological tissues and exploration of their chemical composition with a submicron resolution.…”

“…The objective of this study was to investigate the potential of combined O-PTIR and Raman spectroscopy for analysing breast BMCs, their composition, and the surrounding tissues and to ascertain when this instrument adds additional value over routine vibrational spectroscopic imaging approaches. Furthermore, the complementary nature of stimulated Raman scattering (SRS) and second harmonic generation (SHG) microscopy techniques allow visualisation of the collagen network and the protein distribution in the tissue 50,51 as well as within the BMC with a very good resolution. 54,55 By integrating all these techniques, we aim to gain more information about BMCs in different breast pathologies.…”