Multiple metal tolerance of Paenibacillus dentritiformis isolated from metal contaminated soils west Godavari district (Andhra Pradesh)

Abstract

Sugar industrial effluents possess high amounts of toxic pollutants and contaminate the receiving sites. Treatment of contaminated sites by using microorganisms provides an alternate to conventional methods hence demands in the identification of metal tolerant microorganisms has been increasing day by day. Therefore in this study soil samples collected from Tanuku sugar factory residual effluent point (bank of Gosthani river), west Godavari district A.P were analyzed for the bacterial tolerance to Copper (Cu), Zinc (Zn) and Lead (Pb) in their chloride forms. Additionally, the study was carried out to identify the metal tolerant bacteria by morphological, biochemical and 16S rRNA gene sequencing studies. Four potential bacterial isolates were selected to analyze metal tolerance against CuCl2, ZnCl2, and PbCl2. The sequences were compared with those in NCBI and submitted in gene bank with accession numbers MK100333 (Paenibacillus cookie), MK100334 (Bacillus cereus), MK100335 (Aneurini bacillus sp) and MK100387 (Paenibacillus sp.). A Phylogenetic tree was constructed to Paenibacillus sp. the highly efficient bacterial strain among the four isolates using MEGA 7 soft ware. The results of this study showed that P. dentritiformis had multiple metal tolerances (Cu, Zn and Pb) up to 500mg/L after 72 hrs. The identified bacterial strain proved to be the strong heavy metal tolerant bacterial strain. Hence, its usage will be helpful in the treatment of heavy metals specifically Cu, Zn and Pb contaminated soils and further optimization of these cultures is required to improve its metal resistant capacity.

An immobilized biosorbent from Paenibacillus dendritiformis dead cells and polyethersulfone for the sustainable bioremediation of lead from wastewater

“…shows a phylogenetic tree between the selected strain and other strains. In this regard, Sridevi and Raghuram 39 isolated P. dendritiformis from contaminated soil and deposited it in GenBank under accession number MK100387. This isolate exhibited high tolerance against three metals (Pb > Zn > Cu) at its optimum pH.…”

Tolerance and reduction of Cr(VI) by Bacillus amyloliquefaciens, B. thuringiensis and Paenibacillus sp., isolated from Pasto River

“…presented reduction percentages of 47.5%, 71.7%, and 86.4% in concentrations of 25, 60, and 100 mg/L of Cr (VI), respectively. In Figure 8, could be observed that in the 24-to 60-hour interval, the reduction process was accelerated and the treatment of 100 mg/L of Cr (VI) showed the major percentage indicating that, under stress, this bacterium triggers different mechanisms that could be reduce and block the toxic effect of the metal, increasing its chances of survival in environments contaminated with heavy metals (Ramírez-Díaz, et al, 2008;Wani, et al, 2018;Sridevi and Raghuram, 2019). Additionally, it was determined that the initial Cr (VI) concentration in the control used for this process did not vary significantly; in a certain way, it is confirmed that the bacteria used in the treatment are responsible for the reduction of this metal.…”