Multiple pharmacological and toxicological investigations on Tanacetum parthenium and Salix alba extracts: Focus on potential application as anti-migraine agents

Giacomo, Viviana di; Ferrante, Claudio; Ronci, Maurizio; Cataldi, Amelia; Valerio, Valentina Di; Rapino, Monica; Recinella, Lucia; Chiavaroli, Annalisa; Leone, Sheila; Vladimir‐Knežević, Sanda; Kindl, Marija; Brunetti, Luigi; Menghini, Luigi; Orlando, Giustino

doi:10.1016/j.fct.2019.110783

Cited by 26 publications

(36 citation statements)

References 51 publications

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“…By contrast, the methanol extract displayed a significant stimulating effect on liver DA concentration (Figure 3). This protective effect is consistent with both the antioxidant effect that is exerted by the extract and with literature [22,27]. Overall, the present pharmacological assays suggest that B. speciosa could be considered as a source of natural compounds with potential application in liver inflammation and cancer.…”

Section: Resultssupporting

confidence: 90%

“…This was, at least in part, confirmed by the mild anti-proliferative effect that was exerted by the sole epicatechin (100 µg/mL) (CTR: 100; Epicatechin: 87.88 ± 4.35). On the other hand, we cannot exclude that the stimulating effect induced by methanol extract depends on its major content in phenolic compounds (Tables 1 and 7), with particular regards to gallic acid, which could also exert putative pro-oxidant effects [22,52], thus potentially contributing to the maintenance of a microenvironment favorable to the proliferation of a cancer cell line [24]. Protective effects following extract treatment were also evaluated in a toxicological model constituted by isolated liver specimens that were stimulated with LPS, which increased the 3-HK levels in the liver tissue ( Figure 2).…”

Section: Resultsmentioning

confidence: 96%

“…All of the analytical and chromatographic details are given in Supplemental Materials. B. speciosa water and methanol extracts (5 µg/mL) were also analyzed for accurate phenolic quantitative determination of epicatechin, catechin, and gallic acid while using a reversed phase HPLC-fluorimetric in gradient elution mode, as recently described [22]. The experimental details are given in Supplemental Materials.…”

Section: Phytochemical Compositionmentioning

confidence: 99%

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Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf

et al. 2020

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Bridelia species have been used in traditional African medicine for the management of diverse human ailments. In the current work, the detailed phytochemical profiles of the extracts of the stem bark of B. speciosa were evaluated and the antioxidant and enzyme inhibitory properties of the extracts were assessed. The anti-bacterial and anti-mycotic effects of the extracts were evaluated against selected pathogen strains. Additionally, the anti-proliferative effects were studied on the liver cancer HepG2 cell line. Finally, the putative protective effects were assessed on isolated rat liver that was challenged with lipopolysaccharide (LPS). The results revealed the presence of 36 compounds in the ethyl acetate extract, 44 in the methanol extract, and 38 in the water extract. Overall, the methanol extract showed the highest antioxidant activity, particularly in LPS-stimulated rat liver. Additionally, this extract exerted the highest antimycotic effect on C. albicans, whereas the water extract showed a promising anti-proliferative effect on liver cancer HepG2 cells. The methanol extract was also the most active as enzyme inhibitor, against acetylcholinesterase and butyrylcholinesterase. The current study appraises the antioxidant and enzyme inhibition properties of B. speciosa methanol extract and showed that this specie could be a promising source of biologically active phytochemicals, with potential health uses. Supplementary Materials: The following are available online at http://www.mdpi.com/2076-3921/9/2/128/s1. Supplementary Materials and Methods; CFF-strains. Author Contributions: Conceptualization, C.F. and G.Z.; methodology, G.O.; L.M.; software, L.M.; validation,: C.F.; G.Z.; L.M.; G.O.; formal analysis, C.F.; G.Z.; investigation, K.I.S.; K.B.; S.L.; L.R.; A.C. (Annalisa Chiavaroli); S.V.; P.A.; V.H.; S.C.; R.V.; L.D.L.; A.C. (Alessandro Cama); M.C.N.P.-A.; Z.C.; J.J.; M.F.M.; resources, C.F.; G.O.; L.M.; data curation, C.F.; G.Z.; writing-original draft preparation, M.F.M.; writing-review and editing, C.F., G.Z.; G.O.; L.M.; visualization, L.B.; supervision, L.B.; project administration, C.F.; L.M.; G.O.; G.Z.; funding acquisition, C.F.; G.O.; L.M. All authors have read and agreed to the published version of the manuscript.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 96%

Section: Phytochemical Compositionmentioning

confidence: 99%

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Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf

et al. 2020

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“…The brine shrimp lethality test was performed in order to evaluate extract cytotoxicity. The assay yielded LC 50 values > 100 µg/mL and based on previous studies [13,36], a concentration at least ten fold lower was considered as biocompatibility limit for the subsequent in vitro tests on hypothalamic HypoE22 cells, that were selected as a model of neuronal cell line responding to neuromodulatory and protective agents [17,35]. In the MTT test, HypoE22 cells were stimulated with the extracts in the range 0.1-10 µg/mL and a null effect on cell viability was observed (Figure 2).…”

Section: Resultsmentioning

confidence: 89%

Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts

et al. 2020

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The stem bark (SB) of Piptadeniastrum africanum (PA) has been extensively used in African traditional medicinal systems. However, there is a dearth of scientific information regarding its possible activity in the management of type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. This study therefore attempted to elucidate the in vitro inhibitory action of ethyl acetate, methanol, and water extracts of P. africanum stem bark (PA-SB) on α-amylase, α-glucosidase, acetylcholinesterase, butyrylcholinesterase, and tyrosinase. Cell viability, catecholamine, and 3-hydroxykynurenine levels of hypothalamic HypoE22 cells exposed to PA-SB extracts were also investigated. The phytochemical profiles of the extracts were determined by high performance liquid chromatography (HPLC) and antioxidant properties were investigated. Saponin (867.42 mg quillaja equivalent/g) and tannin (33.81 mg catechin equivalent/g) contents were higher in the methanol extract. Multiple dihydroxy-trimethoxy(iso)flavone isomers, loliolide, eriodictyol, naringenin, luteolin, chrysoeriol, apigenin, and liquiritigenin, were characterized from PA-SB extracts using HPLC. The methanol extract of PA-SB showed highest inhibitory activity against acetylcholinesterase (4.88 mg galantamine equivalent (GALAE)/g extract), butyrylcholinesterase (5.37 mg GALAE/g extract), and tyrosinase (154.86 mg kojic acid equivalent/g extract) while α-glucosidase was effectively inhibited by the ethyl acetate extract (15.22 mmol acarbose equivalent/g extract). The methanol extract of PA-SB also showed potent antioxidant properties (493.87, 818.12, 953.07, and 732.19 mg Trolox equivalent/g extract, for 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays, respectively). PA-SB extracts exhibited antioxidant activity and promising inhibition against key enzymes related to type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. Additionally, all extracts were able to contrast hydrogen peroxide-induced oxidative stress, in HypoE22 cells, thus restoring basal catecholamine and 3-hydroxykinurenine levels, whereas only methanol and water extracts stimulated basal dopamine release. Overall, data from the present study contribute to the biological assessment of P. africanum that appears to be a promising source of natural compounds with protective and neuromodulatory effects.

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“…The comparative 2 −∆∆Ct method was used to quantify the relative abundance of mRNA and then determine the relative changes in individual gene expression (relative quantification) [19]. Finally, an untargeted proteomic analysis was carried out on tissue homogenate [20,21]. The detailed description of RIA, real-time PCR and mass spectroscopy analysis is reported in the Supplementary Materials section.…”

Section: Ex Vivo Studiesmentioning

confidence: 99%

Multidirectional Pharma-Toxicological Study on Harpagophytum procumbens DC. ex Meisn.: An IBD-Focused Investigation

et al. 2020

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In the present study, we investigated the water extract of Harpagophytum procumbens DC. ex Meisn. in an experimental model of inflammatory bowel diseases (IBDs). Additionally, a microbiological investigation was carried out to discriminate the efficacy against bacterial and fungal strains involved in IBDs. Finally, an untargeted proteomic analysis was conducted on more than one hundred colon proteins involved in tissue morphology and metabolism. The extract was effective in blunting the production of oxidative stress and inflammation, including serotonin, prostaglandins, cytokines, and transcription factors. Additionally, the extract inhibited the growth of Candida albicans and C. tropicalis. The extract was also able to exert a pro-homeostatic effect on the levels of a wide plethora of colon proteins, thus corroborating a protective effect. Conversely, the supraphysiological downregulation of cytoskeletal-related proteins involved in tissue morphology and antimicrobial barrier function suggests a warning in the use of food supplements containing H. procumbens extracts.

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Multiple pharmacological and toxicological investigations on Tanacetum parthenium and Salix alba extracts: Focus on potential application as anti-migraine agents

Cited by 26 publications

References 51 publications

Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf

Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf

Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts

Multidirectional Pharma-Toxicological Study on Harpagophytum procumbens DC. ex Meisn.: An IBD-Focused Investigation

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