Multiplex Electrochemiluminescence Polarization Assay Based on the Surface Plasmon Coupling Effect of Au NPs and Ag@Au NPs

Liang, Zihui; Nie, Yixin; Zhang, Xin; Wang, Peilin; Ma, Qingjie

doi:10.1021/acs.analchem.1c00757

Cited by 42 publications

(25 citation statements)

References 36 publications

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“…In the [Ru(bpy) 3 ](PF 6 ) 2 /TPrA system, the signal was generated by the reaction between [Ru(bpy) 3 ](PF 6 ) 2 and TPrA, and the related mechanism was depicted as follows Moreover, the yellow-green light emission at the cathode of BPE was attributed to the ECL reaction of BNQDs-K 2 S 2 O 8 . For the BNQDs/S 2 O 8 2– system, BNQDs were reduced to BN –· , the reduction of S 2 O 8 2– produces the strong oxidant SO 4 –· , and BN –· could react with SO 4 –· to generate the signal. , The proposed ECL process is displayed in the Supporting Information.…”

Section: Results and Discussionmentioning

confidence: 99%

Ultrasensitive and Visual Electrochemiluminescence Ratiometry Based on a Constant Resistor-Integrated Bipolar Electrode for MicroRNA Detection

et al. 2022

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In this work, a new electrochemiluminescence (ECL) platform was constructed for detecting the prostate cancer marker microRNA-141 (miRNA-141) on a constant resistorintegrated closed bipolar electrode (BPE). It consisted of two reservoirs and a constant resistor, and both ends were connected to the anode of the driving electrode and the cathode of BPE. The cathode of BPE was modified with boron nitride quantum dots (BNQDs), and the anode reservoir was the [Ru(bpy) 3 ](PF 6 ) 2 / TPrA system. After introducing a certain amount of hairpin DNA 3 (H3) and ferrocene-labeled single-stranded DNA (Fc-ssDNA) on the surface of the BNQDs, the ECL emission signal of the BNQDs was difficult to be observed by the naked eye, while [Ru(bpy) 3 ]-(PF 6 ) 2 emitted a strong and visible ECL signal. In the presence of the target, bipedal DNA assembled by catalytic hairpin assembly (CHA) took away the Fc-ssDNA and the ECL intensity of the BNQDs was enlarged, and as the concentration of miRNA-141 increased to the cutoff value, yellow-green light was visible by the naked eye. Meanwhile, the red emission signal of [Ru(bpy) 3 ](PF 6 ) 2 /TPrA became weakened. Thus, an ultrasensitive "color switch" ECL biosensor for detection of miRNA-141 was constructed and endowed with a wide linear range from 10 −17 to 10 −7 M and a detection limit of 10 −17 M (S/N = 3). This study provides the potential for investigating portable devices in the detection of lowconcentration nucleic acids.

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Section: Results and Discussionmentioning

confidence: 99%

Ultrasensitive and Visual Electrochemiluminescence Ratiometry Based on a Constant Resistor-Integrated Bipolar Electrode for MicroRNA Detection

et al. 2022

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“…The electromagnetic field coupling between the emission of QDs and the metallic plasmon material appears at different polarization angles under different surface charge distributions . Therefore, the isotropic ECL emission of QDs can be amplified and converted into the directional emission in the presence of metal plasmons Figure demonstrates the effect on the ECL polarization characteristics of SnS 2 QDs with three different Au nanomaterials, including Au NPs, Au NRs, and Au NTs.…”

Section: Resultsmentioning

confidence: 99%

“…30 Therefore, the isotropic ECL emission of QDs can be amplified and converted into the directional emission in the presence of metal plasmons. 10 Figure 4 demonstrates the effect on the ECL polarization characteristics of SnS 2 QDs with three different Au nanomaterials, including Au NPs, Au NRs, and Au NTs. These Au nanomaterials possessed different shapes and ECL enhancement abilities.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Recently, the surface coupling effect with “hot spots” in luminescence enhancement has attracted the attention of researchers. The anisotropy metallic nanostructures with large specific surface areas and sharp tip positions can provide a significantly stronger electromagnetic intensity, which is called the “hot spot” regions. , Metal nanoparticles composed of different elements and the gap coupling distance between Au nanoparticle dimers have further been explored for the research of surface plasmon coupling ECL . More importantly, the anisotropic geometry of the noble metal nanostructure allowed for the excitation of hot spots to effectively modulate the polarization properties of the emitted light .…”

Section: Introductionmentioning

confidence: 99%

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Polarization-Resolved Electrochemiluminescence Sensor Based on the Surface Plasmon Coupling Effect of a Au Nanotriangle-Patterned Structure

et al. 2021

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This work focused on the construction of a nanomaterial-patterned structure for high-resolved ECL signal modulation. Due to the surface coupling effect, the different shapes and distribution states of surface plasmonic nanomaterials not only affect the luminescence intensity enhancement but also decide the electrochemiluminescence (ECL) polarization characteristics. Herein, tin disulfide quantum dots were synthesized via a solvothermal method as ECL emitters. Compared with other nanostructures, Au nanotriangle (Au NT) displayed both the localized surface plasmon resonance electromagnetic enhancement effect and the tip amplification effect, which had significant hot spot regions at three sharp tips. Therefore, self-assembled Au NT-based patterned structures with high density and uniform hot spots were constructed as ideal surface plasmonic materials. More importantly, the distribution states of the hot spots affect the polarization characteristics of ECL, resulting in directional ECL emission at different angles. As a result, a polarization-resolved ECL biosensor was designed to detect miRNA 221. Moreover, this polarization-resolved biosensor achieved good quantitative detection in the linear range of 1 fM to 1 nM and showed satisfactory results in the analysis of the triple-negative breast cancer patients’ serum.

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“…Our previous studies have proved that SEECL were successfully applied for detection carcinoembryonic antigen and Hg 2+ with high sensitivity and selectivity. , Multiplex detection based on electrochemiluminescence is a difficulty that needs to be broken through, and it is also a research hotspot. Normally, electrochemiluminescence multiplex detection require spectrum or potential of the molecules to be separated which limits their application. − As an alternative approach, the spatially resolved electrode array overcomes the shortages, the electrode array was employed to fix specific recognition units such as antibodies or aptamers of different target objects in specific positions of the array, and the targeted detection can be realized. However, SEECL based on photomultiplier tube (PMT) detection can only detect one target at a time, which is not suitable for multiple targets detection.…”

Section: Introductionmentioning

confidence: 99%

Surface-Enhanced Electrochemiluminescence Imaging for Multiplexed Immunoassays of Cancer Markers in Exhaled Breath Condensates

Ding

Huang

et al. 2022

Anal. Chem.

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Recently we have demonstrated that the surface plasmon of noble metal nanoparticles can effectively enhance the ECL intensity of Ru(bpy)3 2+, and we named this detection principle as surface-enhanced electrochemiluminescence (SEECL-I). However, SEECL based on photomultiplier tube (PMT) detection can only detect one target at a time, which is not suitable for multiple targets detection. In this work, we combined our previous developed SEECL with a bioimaging device to develop a novel multiplexed immunassay for simultaneous and fast analysis of cancer markers. A core–shell nanocomposite consisted of gold–silicon dioxide nanoparticles doped with Ru(bpy)3 2+(Au@SiO2-Ru) with strong ECL emission was employed as ECL label due to the localized surface plasmon resonance (LSPR) of AuNPs, which can significantly enhance the ECL emission of Ru(bpy)3 2+. The ECL signals from the 4 × 4 electrode arrays were collected using the constant potential method (current–time curve method) imaging with a sCOMS camera. As a proof-of-concept application, we demonstrated the use of the proposed SEECL-I for simultaneous detection of carcinoembryonic antigen (CEA), neuron specific enolase (NSE), and squamous cell carcinoma antigen (SCC) in exhaled breath condensates (EBCs) with low detection limit (LOD) of 0.17, 0.33, and 0.33 pg/mL (S/N = 3), respectively. The results demonstrated that the proposed SEECL-I strategy can provide a high sensitivity, fast analysis, and high-throughput platform for clinical diagnosis of cancer markers in EBCs.

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Multiplex Electrochemiluminescence Polarization Assay Based on the Surface Plasmon Coupling Effect of Au NPs and Ag@Au NPs

Cited by 42 publications

References 36 publications

Ultrasensitive and Visual Electrochemiluminescence Ratiometry Based on a Constant Resistor-Integrated Bipolar Electrode for MicroRNA Detection

Ultrasensitive and Visual Electrochemiluminescence Ratiometry Based on a Constant Resistor-Integrated Bipolar Electrode for MicroRNA Detection

Polarization-Resolved Electrochemiluminescence Sensor Based on the Surface Plasmon Coupling Effect of a Au Nanotriangle-Patterned Structure

Surface-Enhanced Electrochemiluminescence Imaging for Multiplexed Immunoassays of Cancer Markers in Exhaled Breath Condensates

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