2009
DOI: 10.1016/j.tiv.2009.06.003
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Multiplexed assay panel of cytotoxicity in HK-2 cells for detection of renal proximal tubule injury potential of compounds

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Cited by 73 publications
(67 citation statements)
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“…Interestingly, HK-2 cells, an HPV-immortalized human renal proximal tubule cell line, were shown to have an IC 50 of 20 pg/ml at 72 h posttreatment with Stx2, compared to 2.2 pg/ml and 0.7 pg/ml at the same time point for 2D and 3D NKi-2 cells, respectively, in the present study. HK-2 cells have also been shown to lack sensitivity to drug-induced toxicity (26), indicating that they may not be the best cell line for studying renal cytotoxicity.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Interestingly, HK-2 cells, an HPV-immortalized human renal proximal tubule cell line, were shown to have an IC 50 of 20 pg/ml at 72 h posttreatment with Stx2, compared to 2.2 pg/ml and 0.7 pg/ml at the same time point for 2D and 3D NKi-2 cells, respectively, in the present study. HK-2 cells have also been shown to lack sensitivity to drug-induced toxicity (26), indicating that they may not be the best cell line for studying renal cytotoxicity.…”
Section: Discussionmentioning
confidence: 99%
“…To overcome these limitations, investigators have used either animal models (18,19) or two-dimensional (2D) monolayer cell cultures to study Stx-mediated disease (20)(21)(22). 2D cell culture systems, while simple and low in cost, lack the architecture required to play a major role in cell-cell and cellextracellular matrix (ECM) interactions and are thus incapable of recapitulating the complexity of the in vivo environment (23,24), as well as inadequate at predicting cellular toxicity (25,26). Studies using 2D human renal proximal tubule cells have demonstrated that Stx increases apoptosis in a time-and dose-dependent manner, along with the secretion of IL-1␤, IL-6, and IL-8, the inhibition of protein synthesis, and water absorption (20,21,27,28).…”
mentioning
confidence: 99%
“…Nanoparticles often become enriched in the kidney. Transformed or immortalized animal and human renal proximal tubule cells are frequently used for in vitro nephrotoxicology, and primary proximal tubule cells have also been applied (Chen et al 1990;Bach et al 1997;Pfaller and Gstraunthaler 1998;Li et al 2003Li et al , 2006McGoldrick et al 2003;Pallet et al 2008;Hovda et al 2009;Konigs et al 2009;Wu et al 2009). Herein we investigated the effects of pHF-QDs on well-established porcine and human proximal tubule cell lines, and on primary human proximal tubule cells obtained from different donors and vendors, in order to learn more about the advantages and disadvantages of each cell model.…”
Section: Discussionmentioning
confidence: 99%
“…Also, the use of permanent lines of human cells is not without problems. It has been found that cell lines show reduced sensitivity to toxins and toxic effects of nanoparticles, as compared to primary human cells (Bregoli et al 2009;Wu et al 2009), suggesting that the use of primary human cells might be more appropriate.…”
Section: Introductionmentioning
confidence: 99%
“…Morelli et al [33] validated an HCA screen for phospholipidotic potential of cationic amphiphilic drugs. Application of a multiplex assay analogous to the quadprobe HCA assay with measurements for mitochondrial activity, apoptosis, plasma membrane permeability and DNA in human kidney cells (HK-2) revealed proximal tubule injury potential of compounds [34]. Several studies have demonstrated that neurotoxicity can be effectively screened for using HCA analysis of rat PC12, mouse N1E-115 and human SH-SY5Y neuronal cell lines for assessment of neurite outgrowth [35] and inhibition of cell proliferation [36].…”
Section: M72mentioning
confidence: 99%