MULTIPLIKASI EMBRIO SOMATIS TANAMAN ANGGREK (Dendrobium sp) DENGAN PEMBERIAN KINETIN DAN SUKROSA SECARA IN-VITRO

Abstract

The objective of the study was to obtain the combination of sucrose and kinetin concentration reducted in the best effect on Dendrobium explant. This research was conducted in laboratory of tissues culture in Balai Benih Induk Dinas Pertanian and Peternakan Provinsi Riau Pekanbaru from April to June 2015. The experiment was designed using completely randomized design (CRD), factorial consisting of two factors with three replications, the first factor was sucrose with four level respectively 0, 25, 50,75 g L-1 medium. The second factor was kinetin with four level : 0, 0,1, 1,0, 10 mg L-1. The optimum interaction between effect of sucrose and kinetin for percentage of life explants, age bud formation, percentage of explant forming buds, the number of shoot, a percentage of explant producing root was 50 g L-1 sucrose and 1,0 mg L-1 kinetin. Application of sucrose significantly effect age of bud formation, percentage of explant forming buds, number of shoot, shoots high, percentage of explant producing root, number of root, root high, root and shoot ratio (T/R Ratio). Sucrose at 50 g L-1 medium being the best consentration and Kinetin 1,0 mg L-1 being the best consentration.

Potassium Dihydrogen Phosphate (KH2PO4) and Kinetin Enhance The Growth of Dendrobium Sonia Somatic Embryos

“…The results of this study were compared with research conducted by Heriansyah (2019), then the same results were obtained for the growth of Dendrobium sp. orchid explants, the best results were found in the addition of 1 mg/ L -1 of kinetin media.…”

“…Research on the use of kinetin in tissue culture medium has been carried out by several previous researchers including Bhattacharyya & Banerjee, (2020), Hapsari & Ermayanti (2020), Heriansyah, (2019, Manokari et al (2020), Chawla et al (2020). Meanwhile, the aim of this research was to determine the best concentration of Potassium Dihydrogen Phospate (KH 2 PO 4 ) and Kinetin for the growth of Dendrobium Sonia somatic embryos…”

Multiplication of Dendrobium Sp Orchid Somatic Embries Using In-Vitro Concentrations of MgSO4 and Myo-Inositol in Murashige and Skoog Media

“…The results of this study when compared with research conducted by (Heriansyah et al, 2014), different results were obtained, concluding that the administration of 50 mg/l Myo-inositol into MS media had a significant effect on the growth of the number of shoots of Dendrobium Sp orchid explants with an average -the average number of shoots was 2.11, whereas in this study the administration of the same concentration of 50 mg/l Myo-inositol into MS media produced a better number of shoots of 3.75.…”

“…The results of this study when compared with research conducted by Heriansyah et al (2014), different results were obtained, concluding that the administration of 50 mg/l Myo-inositol into MS media had a significant effect on the height growth of Dendrobium Sp orchid explants with an aver age -the average shoot height was 2.32 cm, while in this study the administration of 150 mg/l Myo-inositol into the MS media was able to produce a shoot height of 1.25 cm, so the results of this study, even though they had used higher concentrations, the results obtained lower.…”

In-Vitro Rooting Induction On The Embryo Somatic Of Dendrobium Species From Riau Province Indonesia

“…Earlier research on the induction of rooting especially in the Dendrobium orchid plant species has not been found, but the research on the use of kinetin on embryo growth that examines kinetin on the growth of embryonic shoots have been done by Heriansyah, 2019. Explaining that the administration of kinetin affects the establishment of a dendrobium of explants species (Heriansyah 2019).…”