Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA

Watcharatanyatip, Kamolwan; Boonmoh, Sirikwan; Chaichoun, Kridsada; Songserm, Taweesak; Woratanti, Mingkhwan; Dharakul, Tararaj

doi:10.1016/j.jviromet.2009.09.027

Cited by 45 publications

(30 citation statements)

References 19 publications

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“…The same was observed with the H7 ELISA (Table 5). The higher relative sensitivity of the ELISA compared to HI test corresponds with the results of other AIV ELISAs [13,15,16,19,35-38]. Hence, it should be considered to replace the more laborious HI test or at least as initial screening in the surveillance [17,34].…”

Section: Discussionmentioning

confidence: 99%

An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies

et al. 2013

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BackgroundAvian influenza virus (AIV) subtypes H5 and H7 attracts particular attention because of the risk of their potential pathogenicity in poultry. The haemagglutination inhibition (HI) test is widely used as subtype specific test for serological diagnostics despite the laborious nature of this method. However, enzyme-linked immunosorbent assays (ELISAs) are being explored as an alternative test method.H5 and H7 specific monoclonal antibodies were experimentally raised and used in the development of inhibition ELISAs for detection of serological response specifically directed against AIV subtypes H5 and H7. The ELISAs were evaluated with polyclonal chicken anti-AIV antibodies against AIV subtypes: H1N2, H5N2, H5N7, H7N1, H7N7, H9N9, H10N4 and H16N3.ResultsBoth the H5 and H7 ELISA proved to have a high sensitivity and specificity and the ELISAs detected H5 and H7 antibodies earlier during experimental infection than the HI test did. The reproducibility of the ELISA’s performed at different times was high with Pearson correlation coefficients of 0.96-0.98.ConclusionsThe ELISAs are a potential alternative to the HI test for screening of large amounts of avian sera, although only experimental sera were tested in this study.

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Section: Discussionmentioning

confidence: 99%

An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies

et al. 2013

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“…Recombinant NP (rNP) was generated and characterized previously by Watcharatanyatip et al (2010). The recombinant NS1 protein (rNS1) was cloned and expressed in this study.…”

Section: Source Of the Recombinant Proteinsmentioning

confidence: 99%

Improved sensitivity of influenza A antigen detection using a combined NP, M, and NS1 sandwich ELISA

Jian-umpunkul

Thepthai

Apiwat

et al. 2012

Journal of Virological Methods

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“…The rapid diagnosis kit such as lateral-flow assay [1,2] on the basis of immunochromatography gives such low sensitivity that it needs over 10 4 PFU/mL, though it has a big advantage in the short detection time, within 30 min [3,4]. Conventional ELISA having the same sensitivity requires a detection time of about 2 h [5,6]. RT-PCR method can detect viruses with high sensitivity and moreover identify the subtype, but needs a detection time of several days, expensive equipment, and skilled technicians to perform [7].…”

Section: Introductionmentioning

confidence: 99%

Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex

et al. 2016

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Recently, point of care testing (POCT) used for diagnosis of influenza infection has a problem showing false negative diagnosis because of the low sensitivity. We would like to report detection of influenza virus A (H1N1) by an immunosensor based on electrochemiluminescence (ECL) that uses an immunoliposome encapsulating tris(2,2'-bipyridyl)ruthenium(II) complex. By using the sensor, we could detect the virus that competed with hemagglutinin (HA) peptide immobilized on self-assembled monolayers (SAMs) in immunoreaction of the antibody bound on the surface of liposome. The HA peptide was 19 mer (TGLRNGITNKVNSVIEKAA). We demonstrated great improvement of sensitivity and accuracy by introducing binary SAMs instead of mono SAMs. The binary SAMs was prepared from 3,3'-dithiodipropionic acid and 1-hexanethiol. Use of the binary SAMs enabled to increase the SAMs coverage on Au electrode; the fact was confirmed by observation of the cathodic desorption currents. By using such an electrode, first the detection method of BSA was optimized to lower ECL background signal. Then we applied the method to the detection of influenza virus. We could successfully detect the virus with higher sensitivity compared with that by POCT and ELISA. The detection range was from a concentration of 2.7 × 10(2) to 2.7 × 10(3) PFU/mL.

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Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA

Cited by 45 publications

References 19 publications

An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies

An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies

Improved sensitivity of influenza A antigen detection using a combined NP, M, and NS1 sandwich ELISA

Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex

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