Multispecific monoclonal antibodies

Ghosh, Souravi; Campbell, Ailsa M.

doi:10.1016/0167-5699(86)90108-8

Cited by 97 publications

(36 citation statements)

References 38 publications

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“…Different antigens may share similar conformational epitopes [43,44]. In contrast, the primary structure of the V H segment of autoantibodies [45] and somatically generated sequences in the H chain CDR3 [46,47] have been shown to be essential in determining the relative degree of polyreactivity of autoantibodies.…”

Section: Discussionmentioning

confidence: 99%

Polyreactivity of Disease‐Associated Anti‐RBC IgG Autoantibodies of Patients with Warm Autoimmune Haemolytic Anaemia and Natural Anti‐RBC IgG Autoantibodies of Healthy Individuals

et al. 2000

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Anti-red blood cell (RBC) immunoglobulin G (IgG) autoantibodies are present in patients with warm autoimmune haemolytic anaemia (WAIHA), and, as natural autoantibodies, in healthy individuals. This study investigated whether the feature of polyreactivity discriminates disease-associated from natural anti-RBC IgG autoantibodies. The patterns of reactivity of puri®ed anti-RBC IgG eluted from the RBC of WAIHA patients and from the RBC of healthy individuals were analysed using quantitative immunoblotting on a panel of whole human tissue or bacterial cell extracts as antigen sources. In parallel, the reactivity patterns of IgG puri®ed from plasma were analysed. Anti-RBC IgG of WAIHA patients and of healthy individuals recognized a wide range of self-and nonself-antigens. The reactivity patterns of anti-RBC IgG were homogeneous among patients and controls, did not differ between patients and controls, and were similar to those of IgG puri®ed from plasma in the case of both patients and healthy individuals. The data demonstrate that the anti-RBC IgG autoantibodies of WAIHA patients share extensive similarity with those of healthy individuals. Polyreactivity is a common feature of both disease-associated and natural anti-RBC IgG autoantibodies.

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Section: Discussionmentioning

confidence: 99%

Polyreactivity of Disease‐Associated Anti‐RBC IgG Autoantibodies of Patients with Warm Autoimmune Haemolytic Anaemia and Natural Anti‐RBC IgG Autoantibodies of Healthy Individuals

et al. 2000

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“…In human hybridoma technology, whether or not EBV transformation is employed, the great majority of immortalised lymphocytes secrete antibody of the IgM isotype (Steel et al, 1977;Chan et al, 1986;Cote et al, 1986). This antibody, being multivalent, can react spuriously with various antigens in a 'multispecific' fashion because any low affinity interactions with such antigens will be amplified by local concentration effects (Ghosh & Campbell, 1986;Campbell et al, 1987). The probability of this irrelevant type of non-specific reaction is increased when antigens have high epitope densities with a repeating structure offering a wide variety of regularly spaced epitopes.…”

Section: Discussionmentioning

confidence: 99%

“…The probability of this irrelevant type of non-specific reaction is increased when antigens have high epitope densities with a repeating structure offering a wide variety of regularly spaced epitopes. Such antigens include single stranded DNA, actin, myosin and vimentin (Ghosh & Campbell, 1986).…”

Section: Discussionmentioning

confidence: 99%

B-Lymphocytes from melanoma patients and normal individuals react with melanoma cells but also with irrelevant antigens

Damato

Campbell²,

McGuire³

et al. 1988

Br J Cancer

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Peripheral B-lymphocytes of 13 patients with uveal melanoma and of 5 healthy individuals were transformed with Epstein-Barr virus (EBV). The reactivity of these transformed cells with autologous or allogeneic melanoma cells and lymphocytes was measured by the enzyme-linked immunosorbent assay (ELISA). Antigens which are neither self nor common environmental antigens (i.e., plant protoplasts, schistosome antigen and keyhole limpet haemocyanin) were used for controls. Lymphocyte reactivity with all types of antigen was apparent both in patients with uveal melanoma and in normal controls. The response detected by the techniques available is likely to reflect antibody multispecificity leading to mis-identification of irrelevant antigens.

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“…The situation is made even more complex by the presence of overlapping idiotopes (17J8) and by inference overlapping paratopes, and paratopes with multi-specificity (19,20). The binding of an anti-idiotype antibody to an idiotope may also change the idiotypic nature (through conformational induced changes) of both the reactants.…”

Section: Idiotopes and Idiotypementioning

confidence: 99%

The nature of immunoglobulin idiotypes and idiotype‐anti‐idiotype interactions in immunological networks

Poskitt¹,

Jean-Francois²,

Turnbull³

et al. 1991

Immunology & Cell Biology

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Multispecific monoclonal antibodies

Cited by 97 publications

References 38 publications

Polyreactivity of Disease‐Associated Anti‐RBC IgG Autoantibodies of Patients with Warm Autoimmune Haemolytic Anaemia and Natural Anti‐RBC IgG Autoantibodies of Healthy Individuals

Polyreactivity of Disease‐Associated Anti‐RBC IgG Autoantibodies of Patients with Warm Autoimmune Haemolytic Anaemia and Natural Anti‐RBC IgG Autoantibodies of Healthy Individuals

B-Lymphocytes from melanoma patients and normal individuals react with melanoma cells but also with irrelevant antigens

The nature of immunoglobulin idiotypes and idiotype‐anti‐idiotype interactions in immunological networks

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