Multispectral system for medical fluorescence imaging

Andersson, Patrik; Montán, S.; Svanberg, Sune

doi:10.1109/jqe.1987.1073216

Cited by 119 publications

(31 citation statements)

References 15 publications

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“…3,4 It can be applied in point monitoring 5 or in imaging applications. 6,7 Applications include diagnostics of human malignant tumors 8,9 and atherosclerotic disease, 10 vegetation and water monitoring, 11,12 analytical chemistry techniques such as HPLC and capillary electrophoresis, 13 and forensic sciences. 14 Fluorescence diagnostics has been largely simplified through the availability of CCD detectors for direct readout of the spectrum obtained in the image plane of a spectrometer.…”

Section: Introductionmentioning

confidence: 99%

Compact fiber-optic fluorosensor using a continuous-wave violet diode laser and an integrated spectrometer

Gustafsson

Pålsson

Svanberg

2000

Review of Scientific Instruments

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A compact fluorosensor with a fiber-optic measurement probe was developed, employing a continuous-wave violet diode laser as an exciting source and an integrated digital spectrometer for the monitoring of fluorescence signatures. The system has the dimensions 22×13×8 cm3, and features 5 nm spectral resolution and an excellent detectivity. Results from measurements on vegetation and human premalignant skin lesions are reported, illustrating the potential of the instrument.

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Section: Introductionmentioning

confidence: 99%

Compact fiber-optic fluorosensor using a continuous-wave violet diode laser and an integrated spectrometer

Gustafsson

Pålsson

Svanberg

2000

Review of Scientific Instruments

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“…Arterial tissue fluorescence has been induced with ultraviolet1'3 and visible laser irradiation,2,4'5 and several classification algorithms have been evaluated for discriminating normal and atherosclerotic arterial tissue. [1][2][3][4][5][6] In these reports, normal and atherosclerotic arterial fluorescence differed, but the cause of this fluorescence difference was not investigated. Fluorescent substances have been extracted from atherosclerotic plaque,7-11 but it is unclear whether these are the fluorophores responsible for the observed differences in the fluorescence of intact normal and atherosclerotic artery.…”

mentioning

confidence: 97%

Biochemical basis for the difference between normal and atherosclerotic arterial fluorescence.

et al. 1989

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The observation that laser-induced fluorescence (LIF) spectra of atherosclerotic and normal artery are different has been proposed as the basis for guiding a "smart" laser angioplasty system. The purpose of this study was to investigate the causes of this difference in LIF. Helium-cadmium laser-induced (325 nm) fluorescence was recorded from pure samples of known constituents of normal and atherosclerotic artery including collagen, elastin, calcium, cholesterol, and glycosaminoglycans. Similarities between the LIF spectra of atherosclerotic plaque and collagen and normal aorta and elastin were noted. LIF spectroscopy was then performed on specimens of atherosclerotic aortic plaque (n=9) and normal aorta (n=13) and on their extracted lipid, collagen, and elastin. Lipid extraction did not significantly alter atherosclerotic plaque or normal aortic LIF, suggesting a minor contribution of lipid to arterial LIF. The LIF spectra of normal aorta wall was similar to the spectra of the extracted elastin, whereas the LIF spectra of atherosclerotic aortic plaque was similar to the spectra of the extracted collagen. These observations are consistent with the reported relative collagen-to-elastin content ratio of 0.5 for normal arterial wall and 7.3 for atherosclerotic plaque. A classification algorithm was developed to discriminate normal and atherosclerotic aortic spectra based on an elastin and collagen spectral decomposition. A discriminant score was formed by the difference of elastin and collagen (E-C) coefficients and used to classify 182 aortic fluorescence spectra. The mean E-C value was +0.83±0.04 for normal and -0.48±0.07 for atherosclerotic aorta (p<0.001). Classification accuracy was 92%. With 325-nm excitation, collagen and elastin are therefore the major fluorophores of aortic atherosclerotic plaque and normal aortic wall, respectively, and the difference between normal and atherosclerotic arterial fluorescence appears to be due to differences in relative collagen and elastin content. Consistent with this observation, a classification algorithm based on a collagen and elastin spectral decomposition can accurately classify normal and atherosclerotic aortic fluorescence spectra. Other laser lines may excite different chromophores. These findings will require validation for muscular arteries. (Circulation 1989;80:1893-1901 The difference between the laser-induced fluorescence spectra of atherosclerotic plaque and normal arterial wall has been well established1-5 and has been proposed as the basis

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“…27, simultaneously imaging recordings also seem quite feasible. The imaged recordings shown in this paper were all obtained by averaging over several laser pulses, while the spectra were recorded with one laser pulse per point.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous multiple species detection in a flame using laser-induced fluorescence

Westblom¹,

Aldén²

1989

Appl. Opt.

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An approach for simultaneous detection of NO, OH and 0 in flames using laser-induced fluorescence is presented. The technique is based on spectral coincidences using a Nd:YAG-based laser system producing a frequency-doubled and frequency-mixed laser beam at 287 and 226 nm, respectively. The possibility of making spatially resolved measurements using a diode-array detector was also investigated. The use of this technique for studying potential laser-induced disturbances was also demonstrated.

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Multispectral system for medical fluorescence imaging

Cited by 119 publications

References 15 publications

Compact fiber-optic fluorosensor using a continuous-wave violet diode laser and an integrated spectrometer

Compact fiber-optic fluorosensor using a continuous-wave violet diode laser and an integrated spectrometer

Biochemical basis for the difference between normal and atherosclerotic arterial fluorescence.

Simultaneous multiple species detection in a flame using laser-induced fluorescence

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