Murine ileitis after intracellular parasite infection is controlled by TGF-β–producing intraepithelial lymphocytes

Buzoni‐Gatel, Dominique; Debbabi, Hajer; Mennechet, Franck J. D.; Martin, Valentina; Lepage, Anne C.; Schwartzman, Joseph D.; Kasper, Lloyd H.

doi:10.1053/gast.2001.22432a

Cited by 88 publications

(89 citation statements)

References 52 publications

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“…TGF-g is a potent immunoregulatory molecule produced by P IEL [26]. Previous studies from our laboratory have shown that the in vivo blockade of TGF-g abrogates adoptive protection in this model of pathogen-driven ileitis [6]. We demonstrate in these studies that the inflammatory profile of LPderived CD4 + T cells is modulated by the adoptive transfer of TGF-g -secreting P IEL.…”

Section: Discussionmentioning

confidence: 51%

“…We have previously shown that IEL are critical effector cells in preventing inflammation in this murine model of T. gondii-driven ileitis [6]. In contrast, the CD4 + T lymphocytes isolated from the LP synergize with intestinal epithelial cells to enhance the pro-inflammatory response against T. gondii [15].…”

Section: Introductionmentioning

confidence: 89%

“…The oral infection of C57BL/6 mice with T. gondii leads to a rapid and severe ileitis with 100% mortality [6,18]. As shown in Fig.…”

Section: Assessment Of the Protective Mechanisms Exerted By Primed Ielmentioning

confidence: 97%

“…Cytokines are essential in the initiation of the inflammatory process after T. gondii infection [6,15,18]. We analyzed the expression of mRNA for a wide panel of intestinal cytokines in mice that received P IEL or U IEL and were then challenged, to evaluate the effect(s) of the IEL adoptive transfer in the recipient intestines.…”

Section: Iel Down-regulate Pro-inflammatory Cytokine Mrna Expression mentioning

confidence: 99%

“…In this context, abnormalities of the IEL population have been reported in a number of human inflammatory bowel diseases (IBD) [1][2][3][4]. Immunoregulatory cytokines, in particular IL-10 and TGF-g , are also important in the control of chronic gut inflammation [5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4⁺ T cells

et al. 2004

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Intraepithelial lymphocytes (IEL) play a key role in gut homeostasis and are critical effector cells preventing the inflammatory intestinal lesions induced in mice following oral infection with Toxoplasma gondii. In this intestinal inflammatory model, CD4 + T lymphocytes from the lamina propria (LP) synergize with the infected enterocytes to secrete pro-inflammatory chemokines and cytokines. In this study, we assessed the mechanisms accounting for the ability of IEL to modulate the inflammatory activity of these cells. Adoptive transfer of IEL purified from wild-type mice, or CD154-,CD95L-or IL-10-deficient mice infected with T. gondii completely impairs the development of the lethal ileitis in recipient mice orally infected with T. gondii. Compared with unprimed IEL isolated from naive mice, the CD8 § g TCR § g subset of primed IEL, isolated from T. gondii-infected mice, secretes increased amount of TGF-g . IEL interact with the LP CD4 + T lymphocytes, down-regulate their production of inflammatory cytokines such as IFN-+ and reduce their proliferative activity. These effects are linked to the secretion of TGF-g and are correlated with a shift in the balance between Smad7/T-bet down-regulation and Smad2/Smad3 up-regulation in LP CD4 + T lymphocytes.

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Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 89%

“…The oral infection of C57BL/6 mice with T. gondii leads to a rapid and severe ileitis with 100% mortality [6,18]. As shown in Fig.…”

Section: Assessment Of the Protective Mechanisms Exerted By Primed Ielmentioning

confidence: 97%

Section: Iel Down-regulate Pro-inflammatory Cytokine Mrna Expression mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4⁺ T cells

et al. 2004

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Innate and T Cell-Mediated Immune Responses in Cryptosporidiosis

Wyatt

McDonald²

2004

World Class Parasites

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A variety of innate immune responses may help to control early parasite replication, initiate inflammation and generate signals for T cell activation. Ultimately, elimination of infection involves cells that are of the Th1 (cell-mediated immunity) phenotype, but there may be a protective role for lymphocytes of the Th2 type (antibody-dependent responses). Intraepithelial lymphocytes have increased activity as a result of infection and may be important in the anti-cryptosporidial immune response.

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Factors determining resistance and susceptibility to infection with Toxoplasma gondii

Suzuki

World Class Parasites

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Murine ileitis after intracellular parasite infection is controlled by TGF-β–producing intraepithelial lymphocytes

Cited by 88 publications

References 52 publications

Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4⁺ T cells

Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4⁺ T cells

Innate and T Cell-Mediated Immune Responses in Cryptosporidiosis

Factors determining resistance and susceptibility to infection with Toxoplasma gondii

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Murine ileitis after intracellular parasite infection is controlled by TGF-β–producing intraepithelial lymphocytes

Cited by 88 publications

References 52 publications

Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4+ T cells

Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4+ T cells

Innate and T Cell-Mediated Immune Responses in Cryptosporidiosis

Factors determining resistance and susceptibility to infection with Toxoplasma gondii

Contact Info

Product

Resources

About

Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4⁺ T cells

Intestinal intraepithelial lymphocytes prevent pathogen‐driven inflammation and regulate the Smad/T‐bet pathway of lamina propria CD4⁺ T cells