Murine orthostatic response during prolonged vertical studies: Effect on cerebral blood flow measured by arterial spin‐labeled MRI

Foley, Lesley M.; Hitchens, T. Kevin; Kochanek, Patrick M.; Mendeloff, John; Jackson, Edwin K.; Ho, Chien

doi:10.1002/mrm.20621

Cited by 32 publications

(36 citation statements)

References 34 publications

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“…Our CBF values in mice at 1.8% isoflurane are 151-254 mL/ 100 g/min, which is consistent with previously reported values of 170-280 mL/100 g/min obtained with single-coil CASL measurement under 2% isoflurane (8). The CBF map also illustrated the spatially heterogeneous CBF distribution in the mouse brain, in which values in the thalamus were consistently greater than those in other regions.…”

Section: Discussionsupporting

confidence: 90%

“…The CBF map also illustrated the spatially heterogeneous CBF distribution in the mouse brain, in which values in the thalamus were consistently greater than those in other regions. This concurs with previous studies in rats, as well as results obtained by single-coil CASL measurement in mice (8,9,27). The single-shot readout scheme used in our study fulfills two major requirements: a reduction in total scan time and mitigation of the transverse decay effect at 9.4 T. The quantitative accuracy of the perfusion measurement is biased by the inclusion of unwanted intravascular spins in the perfusion signal, which are principally found in arteries.…”

Section: Discussionsupporting

confidence: 89%

“…4a. The averaged CBF over these ROIs was 158 AE 36 mL/100 g/ min, which concurs with other studies (8,9). Figure 3c, d shows the corresponding CBF maps obtained with 5% CO 2 -95% air in 1.2% isoflurane and 100% air in 1.8% isoflurane, respectively.…”

Section: Cbf Reacts To Hypercapnia and Anesthesiasupporting

confidence: 89%

“…Assessment of tissue perfusion in the mouse brain by ASL, however, is still challenging because of its low sensitivity, and only a few studies have been published so far (8)(9)(10)(11)(12)(13)(14)(15)(16). As there are a large number of transgenic mouse models of many human brain diseases, including Alzheimer's disease, stroke and other neuronal pathological conditions, there is an important need for an easy and reliable technique to measure CBF in mice.…”

Section: Introductionmentioning

confidence: 99%

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High‐sensitivity cerebral perfusion mapping in mice by kbGRASE‐FAIR at 9.4 T

2010

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The combination of flow-sensitive alternating inversion recovery (FAIR) and single-shot k-space-banded gradient- and spin-echo (kbGRASE) is proposed here to measure perfusion in the mouse brain with high sensitivity and stability. Signal-to-noise ratio (SNR) analysis showed that kbGRASE-FAIR boosts image and temporal SNRs by 2.01 ± 0.08 and 2.50 ± 0.07 times, respectively, when compared with standard single-shot echo planar imaging (EPI)-FAIR implemented in our experimental systems, although the practically achievable spatial resolution was slightly reduced. The effects of varying physiological parameters on the precision and reproducibility of cerebral blood flow (CBF) measurements were studied following changes in anesthesia regime, capnia and body temperature. The functional MRI time courses with kbGRASE-FAIR showed a more stable response to 5% CO(2) than did those with EPI-FAIR. The results establish kbGRASE-FAIR as a practical and robust protocol for quantitative CBF measurements in mice at 9.4 T.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Section: Cbf Reacts To Hypercapnia and Anesthesiasupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High‐sensitivity cerebral perfusion mapping in mice by kbGRASE‐FAIR at 9.4 T

2010

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“…However, distinction between gray and white matter is less clear in mice, and it remains unclear whether the average value obtained from humans applies to mice. Depending on the method used, the BBPC is a linear factor in the calculation of CBF for arterial spin labeling MRI (van Dorsten et al, 1999;Foley et al, 2005;Leithner et al, 2008). Therefore, the erroneous assumption of a BBPC may lead to a proportional error in the absolute quantification of CBF.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Brain–Blood Partition Coefficient for Water in Mice Using MRI

Leithner

Müller

Füchtemeier

et al. 2010

J Cereb Blood Flow Metab

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Cerebral blood flow (CBF) quantification is a valuable tool in stroke research. Mice are of special interest because of the potential of genetic engineering. Magnetic resonance imaging (MRI) provides repetitive, noninvasive CBF quantification. Many MRI techniques require the knowledge of the brain-blood partition coefficient (BBPC) for water. Adopting an MRI protocol described by Roberts et al (1996) in humans, we determined the BBPC for water in 129S6/SvEv mice from proton density measurements of brain and blood, calibrated with deuterium oxide/water phantoms. The average BBPC for water was 0.89 ± 0.03 mL/g, with little regional variation within the mouse brain.

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Sinonasal adenoid cystic carcinoma

Lupinetti

Roberts

Williams

et al. 2007

Cancer

160

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With the increasing number of transgenic mouse models of human brain diseases, there is a need for a sensitive method that allows assessing quantitative whole brain perfusion within a reasonable scan time. Arterial spin labeling (ASL), an MRI technique that permits the noninvasive quantification of cerebral blood flow, has been used to assess rodents brain perfusion. For mice, the reported experiments performed with continuous or pulsed ASL were challenged by poor multislice capability, limited sensitivity, or quantification issues. Here, the recently proposed pseudo‐continuous ASL strategy, which has shown great promise for human studies, was investigated for mouse brain perfusion imaging at 11.75 T. Pseudo‐continuous ASL was experimentally optimized and compared with a standard flow‐sensitive alternating inversion recovery sequence for sensitivity, robustness, absolute quantification, and multislice imaging capability. A sensitivity gain up to 40% and clear advantages for multislice imaging are obtained with pseudo‐continuous ASL. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc.

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Murine orthostatic response during prolonged vertical studies: Effect on cerebral blood flow measured by arterial spin‐labeled MRI

Cited by 32 publications

References 34 publications

High‐sensitivity cerebral perfusion mapping in mice by kbGRASE‐FAIR at 9.4 T

High‐sensitivity cerebral perfusion mapping in mice by kbGRASE‐FAIR at 9.4 T

Determination of the Brain–Blood Partition Coefficient for Water in Mice Using MRI

Sinonasal adenoid cystic carcinoma

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