Murine Th Clones Confer Late Asthmatic Response upon Antigen Challenge

Ohtomo, Takayuki; Kaminuma, Osamu; Kitamura, Noriko; Suko, Matsunobu; Kimura, Noriaki; Mori, Akio

doi:10.1159/000210646

Cited by 9 publications

(8 citation statements)

References 78 publications

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“…Twenty four hours after the final OVA challenge, airway reactivity to aerosolized methacholine was measured using a whole body plethysmograph, as described by Ohtomo et al (2009). In brief, unrestrained conscious mice were placed in whole body plethysmographic chambers, and, after 5 min of stabilization, dose response curves to aerosolized methacholine were generated.…”

Section: Measurement Of Airway Reactivitymentioning

confidence: 99%

Mast cells play a key role in Th2 cytokine-dependent asthma model through production of adhesion molecules by liberation of TNF-α

et al. 2011

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Section: Measurement Of Airway Reactivitymentioning

confidence: 99%

Mast cells play a key role in Th2 cytokine-dependent asthma model through production of adhesion molecules by liberation of TNF-α

et al. 2011

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“…To further analyze mechanisms of LAR, we developed a T-cell-dependent asthma model in which T helper (Th) clones were adoptively transferred into unprimed mice [9]. In addition to eosinophilic inflammation, airway hyperresponsiveness and remodeling [10,11], bronchoconstriction was induced in this model [11,12]. An ovalbumin (OVA)-reactive Th clone, T6-2, conferred LAR, while another Th clone, BF7, induced airway hyperresponsiveness upon OVA challenge [12].…”

Section: Introductionmentioning

confidence: 99%

A Contraction Assay System Using Primary Cultured Mouse Bronchial Smooth Muscle Cells

Kouyama¹,

Otomo-Abe²,

Kitamura³

et al. 2013

Int Arch Allergy Immunol

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Background: Contraction of bronchial smooth muscle is the main mechanism of asthmatic responses. Inflammatory cells such as eosinophils and mast cells produce chemical mediators that induce smooth muscle contraction. To investigate the mechanisms of IgE-independent asthmatic response in murine asthma models, a novel in vitro assay system using primary cultured mouse bronchial smooth muscle cells (BSMC) was explored. Method: Trachea and bronchi were taken from young mice and underwent primary culture. BSMC were expanded in culture and then embedded in a collagen gel. The well-known constrictors leukotriene D₄ (LTD₄), methacholine and histamine were applied to the BSMC gels. The gel images were captured by an image analyzer and contractile responses were evaluated. Results: LTD₄ and methacholine significantly induced the gel contraction in a dose-dependent manner, but histamine did not. Montelukast, a CysLT type ΙΙ receptor antagonist, and atropine, a muscarinic acetylcholine receptor antagonist, inhibited the contractile responses in an agonist-specific manner. Conclusion: A contraction assay system using cultured mouse BSMC was successfully established for the first time. It may go a long way toward identifying bronchoconstricting mediators involved in murine asthma models.

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“…Besides an eosinophilic inflammation, bronchoconstriction was induced in this model [13]. An ovalbumin (OVA)-reactive Th clone, T6–2, conferred LAR, while another Th clone, BF7, induced airway hyperresponsiveness upon OVA challenge [13,14].…”

Section: Introductionmentioning

confidence: 99%

Murine T Cell-Derived Contractile Activity for Bronchial Smooth Muscle Cells

Abe

Koyama

Ohtomo

et al. 2012

Int Arch Allergy Immunol

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Background: Adoptive transfer of helper T (Th) cells conferred a late asthmatic response upon antigen challenge. A possible production of a contractile activity for bronchial smooth muscle (BSM) by activated Th cells was examined. Method: Murine Th clones were stimulated with immobilized anti-CD3 antibody in the presence or absence of anti-CD28 antibody. Culture supernatants were dialyzed and then applied to the collagen gels containing cultured human BSM cells. Results: Culture supernatants of activated but not resting murine Th clones that conferred a late asthmatic response, induced the contraction of human BSM cell-containing collagen gels. Conclusion: Activated Th cells produce a contractile activity for BSM in vitro.

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Murine Th Clones Confer Late Asthmatic Response upon Antigen Challenge

Cited by 9 publications

References 78 publications

Mast cells play a key role in Th2 cytokine-dependent asthma model through production of adhesion molecules by liberation of TNF-α

Mast cells play a key role in Th2 cytokine-dependent asthma model through production of adhesion molecules by liberation of TNF-α

A Contraction Assay System Using Primary Cultured Mouse Bronchial Smooth Muscle Cells

Murine T Cell-Derived Contractile Activity for Bronchial Smooth Muscle Cells

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