Mutagenesis of Saccharomyces cerevisiae by sodium azide activated in Barley

Velemínský, J.; Šilhánková, L.; Smiovská, V.; Gichner, T.

doi:10.1016/0027-5107(79)90126-x

Cited by 28 publications

(4 citation statements)

References 21 publications

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“…Sodium azide is known as a powerful mutagen in several organisms including bacteria, plants and animals (Hadwiger et al, 1976;Veleminsky et al, 1979;Hasegawa and Inoue, 1983;Rines, 1985;Veleminsky and Anglis, 1987;Owais and Kleinhofs, 1988;Raicu and Mixich, 1992;Grant and Salamone, 1994;Sadiq and Owais, 2000). Previous studies clearly showed that the mutagenicity of sodium azide is mediated through the production of an organic metabolite of azide called L-azidoalanine (Ciesla et al, 1980;Owais and Kleinhofs, 1988;Sadiq and Owais, 2000).…”

Section: Discussionmentioning

confidence: 99%

Mutagenic and antimutagenic effects of hexane extract of some Astragalus species grown in the eastern Anatolia region of Turkey

Güllüce¹,

Ağar²,

Barış³

et al. 2010

Phytotherapy Research

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Medical plants and their various extracts have been occasionally used in the treatment of many diseases. Astragalus is one of those medical plants and it has several biological activities. In the present study, the hexane extracts of six Astragalus species, which are grown in the eastern Anatolia region of Turkey, were isolated, and their mutagenic and antimutagenic properties were investigated by using Salmonella typhimurium TA1535, TA1537 and Escherichia coli WP2uvrA tester strains at 0.05, 0.5 and 5 microg/plate concentrations. Known mutagens sodium azide (NaN(3)), 9-Aminoacridine (9-AA) and N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) were used to determine antimutagenic properties of hexane extracts. The results showed that all hexane extracts, investigated in the present study, can be considered genotoxically safe because they do not have mutagenic activity at the tested concentrations. But, a great many of them have antimutagenic activity against 9-Aminoacridine known as a model intercalator agent. The inhibition rates obtained from the antimutagenicity assays ranged from 27.51% (A. macrocephalus--0.05 microg/plate) to 54.39% (A. galegiformis--5 microg/plate). These activities are valuable toward an extension of the employ of these drugs as new phytotherapeutic or preservative ingredients.

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Section: Discussionmentioning

confidence: 99%

Mutagenic and antimutagenic effects of hexane extract of some Astragalus species grown in the eastern Anatolia region of Turkey

Güllüce¹,

Ağar²,

Barış³

et al. 2010

Phytotherapy Research

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“…Mutagenicity of NaN 3 known as a strong mutagen is mediated through the production of an organic metabolite called as L-azidoalanine (Grant and Salamone, 1994;Hadwiger et al, 1976;Hasegawa and Inoue, 1983;Owais and Kleinhofs, 1988;Raicu and Mixich, 1992;Rines, 1985;Sadiq and Owais, 2000;Veleminsky and Anglis, 1987;Veleminsky et al, 1979). In this study, it is observed that the essential oil of the tested flower had antimutagenic activity against NaN 3 at 5 mg/plate concentration (29.49%) and the essential oil of leaves had antimutagenic activity at 5 mg/plate concentration (35.65%).…”

Section: Discussionmentioning

confidence: 65%

Determination of genotoxic and antigenotoxic properties of essential oil from Ferula orientalis L. using Ames/Salmonella and E. coli WP2 bacterial test systems

et al. 2012

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The essential oils having many application fields such as medicine, flavoring, cosmetics are natural products obtained from aromatic plants. As the natural products of Ferula species have a wide range of use in folk medicine, this study was planned to evaluate the mutagenic and antimutagenic activities of essential oils of leaves and flowers of Ferula orientalis grown in Erzurum, through the bacterial reverse mutation assay. Furthermore, the chemical compositions of essential oils isolated by the hyrodistillation method were analysed by gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS), as their biological activities were connected to their contents. According to our results, any tested essential oil at any used concentration on Salmonella typhimurium TA1535 and TA1537 strains and in Escherichia coli WP2 uvrA strain showed no mutagenic activity. However, the tested materials at different concentrations showed antimutagenic activities against the used mutagens. The inhibition rates ranged against sodium azide (NaN3) on S. typhimurium TA1535 from 29% to 36%, against 9-aminoacridine (9-AA) on S. typhimurium TA1537 from 40% to 68% and against N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on E. coli WP2 uvrA from 23% to 52%, respectively. Also, it is revealed by GC and GC/MS analysis of the essential oils isolated from the leaves and flowers, respectively. The major compounds in these oils were determined as α-cadinol, δ-cadinene and germacrene D-4-ol. The results of this study indicate that as the essential oils of F. orientalis have many constituents, they show no mutagenic activity but significant antimutagenic activity, and these materials can be safely used in medicinal applications after further investigations.

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“…Mutagenicity of NaN 3 known as a strong mutagen is mediated through the production of an organic metabolite called as l -azidoalanine (Grant and Salamone, 1994; Hadwiger et al, 1976; Hasegawa and Inoue, 1983; Owais and Kleinhofs, 1988; Raicu and Mixich, 1992; Rines, 1985; Sadiq and Owais, 2000; Veleminsky and Anglis, 1987; Veleminsky et al, 1979). In this study, it is observed that the methanol extracts of the lichens have shown antimutagenic activity against NaN 3 in almost all the concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, it was shown that the tested materials at some concentrations have strong antimutagenic activity in the strains of S. typhimurium (TA1535 and TA1537) and in the E. coli (WP2) against NaN 3 , 9-AA and MNNG, respectively (Baris et al, 2011; Mortelmans and Riccio, 2000;Mortelmans and Zeiger, 2000). Mutagenicity of NaN 3 known as a strong mutagen is mediated through the production of an organic metabolite called as L-azidoalanine (Grant and Salamone, 1994;Hadwiger et al, 1976;Hasegawa and Inoue, 1983;Owais and Kleinhofs, 1988;Raicu and Mixich, 1992;Rines, 1985;Sadiq and Owais, 2000;Veleminsky and Anglis, 1987;Veleminsky et al, 1979). In this study, it is observed that the methanol extracts of the lichens have shown antimutagenic activity against NaN 3 in almost all the concentrations.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic, antigenotoxic and antioxidant properties of methanol extracts obtained from Peltigera horizontalis and Peltigera praetextata

et al. 2013

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Now-a-days, there is a big need to reduce genotoxic effects of mutagenic and carcinogenic agents in environment, which are increased by the technological development. Lichens produce a wide variety of unique metabolites due to being in various extreme areas and being symbiotic organisms of fungi and algae. Therefore, this study was planned to search new sources having antimutagenic activity by researching two different lichen species and to determine whether their usage is safe. With this respect, the mutagenic and antimutagenic properties of methanol extracts of the lichens were determined by the bacterial reverse mutation and sister chromatid exchange assays. Furthermore, the malondialdehyde level, superoxide dismutase, glutathione and glutathione peroxidase activities against aflatoxin B1 were determined for understanding the ways in which the lichens showed their genotoxic properties.

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Mutagenesis of Saccharomyces cerevisiae by sodium azide activated in Barley

Cited by 28 publications

References 21 publications

Mutagenic and antimutagenic effects of hexane extract of some Astragalus species grown in the eastern Anatolia region of Turkey

Mutagenic and antimutagenic effects of hexane extract of some Astragalus species grown in the eastern Anatolia region of Turkey

Determination of genotoxic and antigenotoxic properties of essential oil from Ferula orientalis L. using Ames/Salmonella and E. coli WP2 bacterial test systems

Genotoxic, antigenotoxic and antioxidant properties of methanol extracts obtained from Peltigera horizontalis and Peltigera praetextata

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