Mutagenicity of AH26 in an in vitro mammalian cell mutation assay

Schweikl, Helmut; Schmalz, Gottfried; Stimmelmayr, Horst; Bey, Brigitte

doi:10.1016/s0099-2399(06)80825-3

Cited by 41 publications

(22 citation statements)

References 10 publications

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“…22 (1995) have shown, for AH26 cement, an elevated cytotoxic and mutagenic effect, which was greater for recently mixed material, and reduced significantly after a period of 1 to 7 days. Similar results were observed for the AH26 liquid, or in other words epoxy resin.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of cytotoxicity of two endodontic cements in a macrophage culture

et al. 2005

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ompared to gutta-percha, the endodontic cements are used in small quantity to seal root canals, but are indispensable to achieve hermetically sealed margins, where its biocompatibility depends on the sum of responses of each cell present in the periapical region. The object of this study was to evaluate the cytotoxicity of two endodontic cements, one based on epoxy resin (Sealer 26) and the other containing zinc oxide eugenol (Endofill) by using cultured peritoneal macrophages from Swiss mice to measure the induced production of nitric oxide. After solidification and pulverization, aliquots of 100µl of suspension containing 18mg/mL of the respective cements were added to 96-well tissue culture plates containing the tissue culture of macrophages at a concentration of 5.0X10 6 cells/ml. In the positive control group the cell culture was treated with 10mg/mL of lipopolyssaccharide from Escherichia coli 026:B6 and the cell culture alone represented the negative control. After 48 hours of incubation, at 37 º C, in 5% CO 2 , the cultures were placed in an ELISA automatic reader to evaluate the release of nitric oxide. The production of nitric oxide for cement Sealer 26 was between 36.1 and 313.0 µmols, with a mean of 143.82+111.03µmols, while for the Endofill these values were significantly less (p=0.01), varying from 50.8 to 125.7µmols, with a mean of 80.33+28.42 µmols. In the positive and negative control groups the mean release of nitric oxide was of 162.75µmols and 4.42µmols, respectively. There was no significant difference between the positive control group and cement Sealer 26 (p>0.05). Therefore, the cement Sealer 26 caused significantly greater toxicity to the macrophages, possibly due to the components from the epoxy resin and formaldehyde release during polymerization. Uniterms: Root canal filling materials; Nitric oxide; Macrophages. omparativamente à guta-percha, os cimentos endodônticos são utilizados em pequena quantidade nas obturações dos canais radiculares, mas são imprescindíveis para obtenção do hermético selamento marginal, sendo sua biocompatibilidade dependente do somatório das respostas de cada célula presente na região periapical. Por conseguinte, o objetivo deste estudo foi avaliar em cultura de macrófagos peritoneais de seis camundongos Swiss, a citotoxicidade de dois cimentos endodônticos, um à base de resina epóxica (Sealer 26) e outro contendo óxido de zinco e eugenol (Endofill), quanto a indução da produção de óxido nítrico. Após a presa e pulverização, alíquotas de 100µl da suspensão contendo 18mg/mL dos respectivos cimentos foram adicionados em placa de cultura de tecido de 96 poços, contendo a cultura de macrófagos na concentração de 5,0X10 6 células/ml. Após 48 horas de incubação, a 37 º C, em 5% de CO 2 , foi feita a leitura em um leitor ELISA automático para se averiguar a liberação de óxido nítrico.

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Section: Discussionmentioning

confidence: 97%

Evaluation of cytotoxicity of two endodontic cements in a macrophage culture

et al. 2005

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“…The cytotoxicity due to formaldehyde is supported by other researches 20,21) Additionally, mutagenic substances, epoxy derivatives of bisphenol-A-diglicidylether which may be also cytotoxic, have been found in the set material 20,22) . It is known that epoxy-bis-phenol resin (liquid component of AH 26) relate to its severe cytotoxicity 23) . So other researcher pointed to the possibility that mixed silver-free AH 26 might contain small amounts of two mutagenic substances : bisphenol A diglycidyl eter and formaldehyde 24) .…”

Section: ⅳ Discussionmentioning

confidence: 99%

In vivo study on the biocompatibility of new resin-based root canal sealers

Kim

Baek

Bae

2002

J Korean Acad Conserv Dent

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“…Schwikl et al concluded that mixed AH26 was mutagenic, and the genotoxicity depended on setting time. 48 Physiological saline eluates of mixed AH26 were not mutagenic. Leyhausen et al showed that AH-plus revealed no genotoxicity and mutagenicity.…”

Section: Genotoxicity Of Root Canal Sealersmentioning

confidence: 97%

“…53 The leakage of formaldehyde from the epoxy sealers has been contributed to the mutagenic effects. 48,54 Formaldehyde is carcinogenic in animals; however, there are only few evidences for showing carcinogenicity in human. 55 Considering the low exposure of these materials from epoxy sealers, it seems that such sealers do not contribute to increased risk of carcinogenicity in human.…”

Section: Genotoxicity Of Root Canal Sealersmentioning

confidence: 99%