Mutagenicity of ozone in different repair-deficient strains of Escherichia coli

L'Hérault, Pierre; Chung, Young Sup

doi:10.1007/bf00329945

Cited by 16 publications

(3 citation statements)

References 48 publications

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“…Although ozone and singlet oxygen have both been shown to cause DNA damage in vivo and in vitro, their roles in oxidative mutagenesis remain controversial (51,63,68,145,199,230).…”

Section: Oxidative Damage and Repairmentioning

confidence: 99%

Oxidative stress responses in Escherichia coli and Salmonella typhimurium.

Farr

Kogoma

1991

Microbiological Reviews

675

373

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INTRODUCTION AND SCOPE OF REVIEW been shown to cause damage to DNA, RNA, protein, and lipids. Active oxygen species are produced as an ines-Oxidative stress can be functionally defined as an excess capable by-product of normal aerobic metabolism, and their of prooxidants in the cell. Active oxygen molecules have production is further enhanced by exposure to certain environments or by dietary or disease conditions. Oxygen toxicity results wh~en the-degree of oxidative stress exceeds the * Corresponding author. capacity of the cell defense systems. Oxidative stress is 561

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“…Although ozone and singlet oxygen have both been shown to cause DNA damage in vivo and in vitro, their roles in oxidative mutagenesis remain controversial (51,63,68,145,199,230).…”

Section: Oxidative Damage and Repairmentioning

confidence: 99%

Oxidative stress responses in Escherichia coli and Salmonella typhimurium.

Farr

Kogoma

1991

Microbiological Reviews

675

373

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show abstract

“…Most, if not all, living organisms require defensive systems to protect against active oxygen species. Substantial attention has been paid to the relative roles of superoxide (O2-), hydrogen peroxide (H202), the hydroxyl radical (OH'), and singlet oxygen in mediating, directly or indirectly, damage to cellular components (4,6,12,23,25,32,37). Parallel attention has been paid to the roles of enzymes that may function as protective scavengers of active oxygen species.…”

mentioning

confidence: 99%

Toxicity and mutagenicity of plumbagin and the induction of a possible new DNA repair pathway in Escherichia coli

Farr¹,

Natvig²,

Kogoma³

1985

J Bacteriol

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Actively growing Escherichia coli cells exposed to plumbagin, a redox cycling quinone that increases the flux Of O2 radicals in the cell, were mutagenized or killed by this treatment. The toxicity of plumbagin was not found to be mediated by membrane damage. Cells pretreated with plumbagin could partially reactivate lambda phage damaged by exposure to riboflavin plus light, a treatment that produces active oxygen species. The result suggested the induction of a DNA repair response. Lambda phage damaged by H202 treatment were not reactivated in plumbagin-pretreated cells, nor did H202-pretreated cells reactivate lambda damaged by treatment with riboflavin plus light. Plumbagin treatment did not induce lambda phage in a lysogen, nor did it cause an increase in 3-galactosidase production in a dinD::Mu d(lac Ap) promoter fusion strain. Cells pretreated with nonlethal doses of plumbagin showed enhanced survival upon exposure to high concentrations of plumbagin, but were unchanged in their susceptibility to far-UV irradiation. polA and recA mutants were not significantly more sensitive than wild type to killing by plumbagin. However, xth-1 mutants were partially resistant to plumbagin toxicity. It is proposed that E. coli has an inducible DNA repair response specific for the type of oxidative damage generated during incubation with plumbagin. Furthermore, this response appears to be qualitatively distinct from the SOS response and the repair response induced by H202. 1309 on July 16, 2020 by guest

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“…In this regard, therefore, nonthermal plasma mutagenesis shows special advantages for it may overcome the abovementioned shortcomings, and in fact, it has currently gained increasing attention for its usage in mutagenesis [ 33 ]. In nonthermal plasma, in addition to the critical effects of charged particles and the induced reactive species [ 34 ], other factors, such as UV [ 35 , 36 ] and ozone [ 37 , 38 ], may also play important roles in the induction of gene mutations and the improvement of mutagenesis efficiency. In particular, as one of the popular plasma techniques, dielectric barrier discharge (DBD) nonthermal plasma has emerged to be an effective approach for microbe mutagenesis because it does not require a vacuum system and is characterized by a low temperature treatment, high concentration of active species, good uniformity of discharge, simple operation, rapid mutation, high mutation rate and strong controllability [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy

Zhang

et al. 2018

PLoS ONE

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As a traditional Chinese medicine, Ganoderma lingzhi has attracted increasing attention for both scientific research and medical application. In this work, in order to improve the production of polysaccharides from an original wide-type (WT) strain (named "RWY-0") of Ganoderma lingzhi, we applied atmospheric-pressure dielectric barrier discharge (DBD) nonthermal plasma to the protoplasts of RWY-0 for mutagenesis treatment. Through a randomly amplified polymorphic DNA (RAPD) assay, at least 10 mutagenic strains were confirmed. They also showed different mycelium characteristics in terms of shape, color, size and biomass in liquid fermentation. The mutant strains were examined by infrared spectroscopy, and based on the established near-infrared (NIR) quantification model, the polysaccharide contents in these mutants were quantitatively evaluated. As a result, we found that the Ganoderma polysaccharide contents in some of the mutant strains were significantly changed compared with that of the original WT strain. The polysaccharide content of RWY-1 G. lingzhi was considerably higher than that of the WT strain, with an increase of 25.6%. Thus, this preliminary work demonstrates the extension of the plasma mutagenesis application in acquiring polysaccharide-enhanced Ganoderma lingzhi mutants and shows the usefulness of NIR spectroscopy in the rapid screening of mutagenic strains for other important ingredients.

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Mutagenicity of ozone in different repair-deficient strains of Escherichia coli

Cited by 16 publications

References 48 publications

Oxidative stress responses in Escherichia coli and Salmonella typhimurium.

Oxidative stress responses in Escherichia coli and Salmonella typhimurium.

Toxicity and mutagenicity of plumbagin and the induction of a possible new DNA repair pathway in Escherichia coli

Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy

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