Mutation breeding by ion implantation

Zhang, Yu; Deng, Jing; He, Jianguo; Yuping, Huo; Wu, Yuejin; Wang, Xuedong; Lui, Guifu

doi:10.1016/0168-583x(91)95307-y

Cited by 109 publications

(31 citation statements)

References 2 publications

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“…At present, there are many mutagenic sources, such as ultraviolet rays (Said et al 2010), γ-rays (Fatnassi et al 2011), laser irradiation and the N + ion beam . N + ion beam implantation has been increasingly used in various fields, especially in industrial microbial mutagenesis and mutation breeding (Yu et al 1991). This is due to the controllable damage rate, higher mutation rate, and wider spectrum of mutations obtained by N + ion beam implantation compared to traditional mutation methods (Feng et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Song¹,

Liu²,

Wang³

et al. 2014

Braz. arch. biol. technol.

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Section: Introductionmentioning

confidence: 99%

Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Song¹,

Liu²,

Wang³

et al. 2014

Braz. arch. biol. technol.

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“…Plant tissues Plant tissues Plant tissues Plant tissues Table 1 shows a list of the plant species, mostly horticultural ones, and tissue culture-derived explants used in this study. The explants were rehydrated in sterile distilled water for 30 min, thereafter cultured onto artificial media [Murashige and Skoog (MS) 11 + NAA and kinetin each at 0.5 mg/l for items 1 and 16 (Table 1), White 12 for items 2 and 6-8, MS+NAA and kinetin each at 1.0 mg/l for items 3 and 4, MS + BA 1 mg/l for items 5 and 17, Vacin and Went (VW) 13 + 20% coconut water for items 9-14, and MS supplemented with BA, GA 3 and NAA at 1, 0.1 and 0.01 mg/l, respectively, for items 15, 18 and 19] kept at 28±1 o C under continuous light approximately at 13 µmol/m 2 / s for 15 days. The fresh onion outermost cell layer from its bulb scale (uncultured) was also used in this study.…”

Section: −10mentioning

confidence: 99%

“…In ion-beam mutagenesis a large number of cells are irradiated and DNA modifications are randomly induced in the nucleus, of which the desired ones are subsequently selected out. 3 In ion-beam-induced DNA transfer only the cell envelope is bombarded in order to allow a subsequent transfer of whole DNA into the internal cell region. 7 A recent report 10 described the interaction of energetic ions with bacterial cells, inducing direct DNA transfer into E. coli, indicating that ion beams with an energy such that the ion range is , but none of them has been well supported experimentally.…”

Section: −10mentioning

confidence: 99%

“…The technology is typified by ion bombardment, a physical process in which energetic charged particles are accelerated by an electric field and transported to a target into which they penetrate, introducing foreign atoms, electric charge, and radiation damage in the near surface region. 1 Heavy ion beams have recently been used to bombard biological materials for genetic modification purposes, particularly for the mutation of plants and bacteria [2][3][4][5][6] , in which the DNA structure is modified by relatively high energy (~10 2 …”

Section: Introductionmentioning

confidence: 99%

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Apavatjrut¹,

Alisi²,

Phanchaisri³

et al. 2003

ScienceAsia

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Although the technology of ion-beam-induced gene transfer into either plant or bacterial cells has been successfully established, relevant mechanisms have not been understood. This work aimed to study the process of induction and thus to develop applications of ion beam bioengineering. Cells of various plant tissues were bombarded in vacuum with argon and nitrogen ion beams at energies of 15-30 keV with fluences ranging from 5 × 10 14 -3 × 10 16 ions/cm 2 . The ion bombardment effects on tissue viability and neutral red dye molecule transfer into the cells through the cell envelope were investigated. The results showed that the characteristics of the tissue survival from the ion bombardment and penetration of the dye molecules into the cells through the cell envelope depended on ion species, energy and fluence. For 30-keV argon-ion bombardment at a fluence of 2 × 10 15 ions/cm 2 , the dye molecules entered the cells without fatal injury, whereas under other conditions, the dye either did not enter the cells or stained the nuclei. On the cell envelope surface, ion-bombardment-induced crater-like structures were observed. Calculations indicated that exogenous molecule transfer into living plant cells can be achieved by ion beams with appropriate physical parameters such that the ion range and the radiation damage range lie within the solid cell wall thickness.

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“…The low-energy ion beam irradiation (10 200 keV) has been approved to have a wide range of biological effects in recent years (Yu and Deng, 1991;Chen et al, 1998;Wu and Yu, 2001;Phanchaisri et al, 2002;Yu et al, 2006;Liu et al, 2007). Meanwhile, there were some arguments on the mechanism of these biological effects because of their short penetrating and their irradiation needing desiccation and vacuum conditions (Phanchaisri et al, 2002;Yu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

SOS induction by vacuum, desiccation and low-energy ion beam mock-irradiation in bacteria

et al. 2009

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In this work, we compared the SOS response induced by vacuum-drying, desiccation (wind-spray-drying) and low-energy ion beam mock-irradiation with that of mitomycin C, UV induction. The induction factor induced by vacuum-drying and low-energy ion beam mock-irradiation was relatively higher than that of desiccation in Sal94 and JC19008 strain, respectively. These findings revealed that the SOS response produced by low-energy ion beam mock-irradiation was mainly induced by the step of vacuum-drying, unlikely by the step of wind-spray-drying. The mutation frequencies of rifampicin resistance gene in AB1157 and lacI gene in W3110 increased significantly by vacuum treatment and low-energy ion beam mock-irradiation, but had no remarkable change by desiccation treatment. Meanwhile, the mutation frequency of rifampicin resistance gene in IC400 strain was not significantly influenced by these treatments. These results implied that the SOS response played an important role in the mutations induced by vacuum treatment and low-energy ion beam mock-irradiation.

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Mutation breeding by ion implantation

Cited by 109 publications

References 2 publications

Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Untitled

SOS induction by vacuum, desiccation and low-energy ion beam mock-irradiation in bacteria

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