Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, Bombyx mori

Wang, Lingyan; Kiuchi, Takashi; Fujii, Tsuguru; Daimon, Takaaki; Li, Muwang; Banno, Yutaka; Kikuta, Shingo; Kikawada, Takahiro; Katsuma, Susumu; Shimada, Toru

doi:10.1016/j.ibmb.2013.03.011

Cited by 53 publications

(47 citation statements)

References 47 publications

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“…Interestingly, the "extra" White homologs of Anopheles and Aedes fall into the Scarlet group (a result also seen in Wang et al 2013), and we call them "Scarlet-like" to distinguish them from the Scarlet orthologs in this group.…”

Section: Phylogenetic Analysis Of Abc-transporter Eye-color Homologsmentioning

confidence: 76%

“…While not necessary for eye color, BmOk is necessary for the proper importation of uric acid into the larval integument (Wang et al 2013), a role that is also dependent on the proper function of the Bombyx w ortholog, Bmw3 (Abraham et al 2000;Komoto et al 2009;Tatematsu et al 2011). Interestingly, Anopheles mosquito larvae also use uric acid to pigment their integuments, a function dependent on mosquito W orthologs, while mutations in a locus called collarless prevent proper importation of uric acid without affecting eye color (Benedict et al 1996a(Benedict et al ,b, 2003.…”

Section: Discussionmentioning

confidence: 99%

“…While this conclusion has also been supported by an apparent lack of a Tribolium bw ortholog, bw orthologs have been identified in other species that also lack pteridine eye pigments (Tatematsu et al 2011;Wang et al 2013). Furthermore, Bw is known in Drosophila to play a role in other tissues (Campbell and Nash 2001;Borycz et al 2008), so it would not be surprising for the Tribolium lineage to have maintained an ortholog of this gene.…”

Section: Identification Of Tcbwmentioning

confidence: 98%

“…More interestingly, knockdown of the putative Tcst also caused a white-eye phenocopy, while control experiments with pteridine biosynthesis enzymes did not alter eye color or pigmentation of any other examined tissue (Broehan et al 2013). Furthermore, to date, no study has identified a Tribolium ortholog of bw (Tcbw) (Tatematsu et al 2011;Broehan et al 2013;Wang et al 2013). Altogether, these results suggest that the visible pigments of Tribolium eyes are composed only of ommochromes.…”

mentioning

confidence: 90%

“…Furthermore, our annotations showed that, in each of these species, this fourth gene was located next to the brown ortholog in a tail-to-tail formation, suggesting a common origin. A characterization of the Bombyx gene has since been reported (Wang et al 2013), and it was given the name Bmok. This same study also identified the extra Apis transporter as an ok ortholog (Amok) and noted the tandem arrangement in both species.…”

Section: Phylogenetic Analysis Of Abc-transporter Eye-color Homologsmentioning

confidence: 99%

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The ABCs of Eye Color inTribolium castaneum: Orthologs of theDrosophila white,scarlet, andbrownGenes

et al. 2015

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In Drosophila melanogaster, each of the three paralogous ABC transporters, White, Scarlet and Brown, is required for normal pigmentation of the compound eye. We have cloned the three orthologous genes from the beetle Tribolium castaneum. Conceptual translations of Tribolium white (Tcw), scarlet (Tcst), and brown (Tcbw) are 51, 48, and 32% identical to their respective Drosophila counterparts. We have identified loss-of-eye-pigment strains that bear mutations in Tcw and Tcst: the Tcw gene in the ivory (i) strain carries a single-base transversion, which leads to an E / D amino-acid substitution in the highly conserved Walker B motif, while the Tcst gene in the pearl (p) strain has a deletion resulting in incorporation of a premature stop codon. In light of these findings, the mutant strains i and p are herein renamed white ivory (w i ) and scarlet pearl (st p ), respectively. In addition, RNA inhibition of Tcw and Tcst recapitulates the mutant phenotypes, confirming the roles of these genes in normal eye pigmentation, while RNA interference of Tcbw provides further evidence that it has no role in eye pigmentation in Tribolium. We also consider the evolutionary implications of our findings.

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Section: Phylogenetic Analysis Of Abc-transporter Eye-color Homologsmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Identification Of Tcbwmentioning

confidence: 98%

mentioning

confidence: 90%

Section: Phylogenetic Analysis Of Abc-transporter Eye-color Homologsmentioning

confidence: 99%

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The ABCs of Eye Color inTribolium castaneum: Orthologs of theDrosophila white,scarlet, andbrownGenes

et al. 2015

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RNA interference‐mediated knockdown of eye coloration genes in the western tarnished plant bug (Lygus hesperus Knight)

Brent

Hull

2018

Arch Insect Biochem Physiol

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Insect eye coloration arises from the accumulation of various pigments. A number of genes that function in the biosynthesis (vermilion, cinnabar, and cardinal) and importation (karmoisin, white, scarlet, and brown) of these pigments, and their precursors, have been identified in diverse species and used as markers for transgenesis and gene editing. To examine their suitability as visible markers in Lygus hesperus Knight (western tarnished plant bug), transcriptomic data were screened for sequences exhibiting homology with the Drosophila melanogaster proteins. Complete open reading frames encoding putative homologs for all seven genes were identified. Bioinformaticbased sequence and phylogenetic analyses supported initial annotations as eye coloration genes. Consistent with their proposed role, each of the genes was expressed in adult heads as well as throughout nymphal and adult development. Adult eyes of those injected with double-stranded RNAs (dsRNAs) for karmoisin, vermilion, cinnabar, cardinal, and scarlet were characterized by a red band along the medial margin extending from the rostral terminus to the antenna. In contrast, eyes of insects injected with dsRNAs for both white and brown were a uniform light brown. White knockdown also produced cuticular and behavioral defects. Based on its expression profile and robust visible phenotype, cardinal would likely prove to be the most suitable marker for developing gene editing methods in Lygus species.

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Comparative genomic analysis of ABC transporter genes inTenebrio molitorand four other tenebrionid beetles (Coleoptera: Tenebrionidea)

Xiao

Yang

et al. 2022

Arch Insect Biochem Physiol

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ATP-binding cassette (ABC) transporters, one of the largest transmembrane protein families, transport a diverse number of substate across membranes. Details of their diverse physiological functions have not been established. Here, we identified 87 ABC transporter genes in the genomes of Tenebrio molitor along with those from Asbolus verrucosus (104), Hycleus cichorii (65), and Hycleus phaleratus (80).Combining these genes (336 in total) with genes reported in Tribolium castaneum (73), we analyzed the phylogeny of ABC transporter genes in all five Tenebrionids. They are assigned into eight subfamilies (ABCA-H). In comparison to other species, the ABCC subfamily in this group of beetles appears expanded. The expression profiles of the T. molitor genes at different life stages and in various tissues were also investigated using transcriptomic analysis. Most of them display developmental specific expression patterns, suggesting to us their possible roles in development. Most of them are highly expressed in detoxification-related tissues including gut and Malpighian tubule, from which we infer their roles in insecticide resistance. We detected specific or abundant expressions of many ABC transporter genes in various tissues such as salivary gland, ovary, testis,

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Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, Bombyx mori

Cited by 53 publications

References 47 publications

The ABCs of Eye Color inTribolium castaneum: Orthologs of theDrosophila white,scarlet, andbrownGenes

The ABCs of Eye Color inTribolium castaneum: Orthologs of theDrosophila white,scarlet, andbrownGenes

RNA interference‐mediated knockdown of eye coloration genes in the western tarnished plant bug (Lygus hesperus Knight)

Comparative genomic analysis of ABC transporter genes inTenebrio molitorand four other tenebrionid beetles (Coleoptera: Tenebrionidea)

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