Mutations affecting levels of tetrahydrofolate interconversion enzymes in Saccharomyces cerevisiae

Jones, Elizabeth W.; Lam, Keng-Bon

doi:10.1007/bf00271239

Cited by 18 publications

(12 citation statements)

References 20 publications

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“…Yeast cells were grown either in YEPD (1% yeast extract, 2% peptone, 2% dextrose) or in defined synthetic media supplemented with 2% dextrose as a carbon source (26 Nucleic acid preparation. Bacterial plasmid DNA was prepared as described by Woolford and Rosbash (73).…”

Section: Methodsmentioning

confidence: 99%

Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene.

Larkin¹,

Thompson²,

Woolford³

1987

Mol. Cell. Biol.

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The Saccharomyces cerevisiae CRY] gene encodes ribosomal protein rp59, a component of the 40S ribosomal subunit. Mutations in CRY] can confer resistance to the alkaloid cryptopleurine, an inhibitor of the elongation step of translation. The nucleotide sequence of the cloned CRY] gene was determined. The predicted amino acid sequence shows that CRY] encodes a 14,561-dalton polypeptide that has 88% amino acid sequence homology to the hamster or human S14 ribosomal protein responsible for emetine resistance and 45% homology to Escherichia coli ribosomal protein Sll. Analysis of the DNA sequences upstream from CRYI revealed the presence of three sequences, HOMOLl (consensus, A/TACATCC/TG/ATA/GCA), RPG (consensus, ACCCA/ GTACATT/CT/A), and a thymine-rich sequence, found upstream of more than 20 other cloned yeast genes encoding components of the translational apparatus. We exploited the ability to assay the expression of CRY) in vivo by using the cryptopleurine resistance phenotype to demonstrate that these three consensus sequences are necessary for the transcription of CRY). We previously showed that the upstream promoter element of the yeast RP39A gene consists of these identical sequence motifs. Therefore, we suggest that these three sequences define a consensus promoter element for the genes encoding the yeast translational apparatus. CRY) is one of several hundred yeast genes, including ribosomal protein genes, whose expression is transiently decreased 10-fold upon heat shock. We found that the HOMOLl and RPG consensus sequences are not necessary for the heat shock response of CRY).The biosynthesis, assembly, and function of ribosomes are complicated interrelated processes essential to the function and growth of all cells. Equimolar quantities of more than 70 different ribosomal proteins synthesized in the cytoplasm and four ribosomal RNAs transcribed in the nucleus are assembled into ribosomes in the nucleolus (16). Ribosome biosynthesis is tightly coordinated with the physiological state of the cells. In the yeast Saccharomyces cerevisiae, the rate of synthesis of ribosomal proteins is coordinately decreased in response to heat shock (31) or upon sporulation (33) and is increased when the yeast is shifted from medium containing ethanol as a carbon source to medium containing glucose as a carbon source (29). To study the coordinate expression of ribosomal genes and the role of individual ribosomal components in yeast ribosome assembly and function, we and other workers have cloned a number of yeast ribosomal protein genes (4, 5, 13-15, 22, 35, 37, 39, 60, 72). With few exceptions, these genes are unlinked, occur in two copies in the genome, and contain a single intervening sequence (1, 4, 5, 13-16, 28, 35, 48, 72, 73).Experiments thus far indicate that a variety of control mechanisms operate to coordinate yeast ribosome biosynthesis. Ribosomal protein mRNA transcription, processing, stability, and translation are regulated, as well as the stability of the ribosomal proteins themselves (11, 12, 18, 19, 29-31,...

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Section: Methodsmentioning

confidence: 99%

Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene.

Larkin¹,

Thompson²,

Woolford³

1987

Mol. Cell. Biol.

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“…HML and glkl have both been mapped against his4 and leu2 and are VOL. 44,1980 on September 22, 2020 by guest http://mmbr.asm.org/ Downloaded from…”

Section: Chromosome Mmentioning

confidence: 99%

“…Gene cly7 is linked to ural but fails to show VOL. 44,1980 on September 22, 2020 by guest http://mmbr.asm.org/ Downloaded from linkage to fasl (111). Therefore, cly7 and fasl were placed on opposite ends of this segment.…”

Section: Chromosome XImentioning

confidence: 99%

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Genetic map of Saccharomyces cerevisiae.

Mortimer¹,

Schild²

1980

Microbiological Reviews

205

112

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“…Thus it seems very easy to recover ade3 mutants and a remarkably high number of independently arising ade3 mutants were found. In four of the experiments the thermal selection was combined with selection against ade3 (26): The cells were grown in SD + adenine + histidine prior to the heat treatment in order to make the ade3 mutants heat sensitive. In experiment 10 which included selection against ade3 it is seen that the thermal selection is not completely efficient: Despite the selection against Ade-mutants a red colony appeared which turned out to be an adel mutant.…”

Section: Characterization Of Mutantsmentioning

confidence: 99%

Genetic and molecular characterization of a distiller’s yeast

Keiding

1985

Carlsberg Res. Commun.

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Auxotrophic mutants have been induced by UV-irradiation and EMS treatment in meiotic segregants of a distiller's production strain of yeast, In complementation tests some of the mutants were allelic to adel, ade3, lys2, met13, trpl, ural and ura4 of standard strains orS. cerevisiae.Crosses were made between two spore-derived clones of the distiller's strain and haploid S. cerevisiae strains carrying several auxotrophic markers. Tetrad analysis of the mating products revealed that the spore-derived clones were disomic for six and seven chromosomes, respectively.Chromosomes III from the distiller's production strain were compared to their counterparts in standard strains ofS. cerevisiae at several loci. Cloned DNA from HIS4, LEU2 and MATloci from the standard strain were used as hybridization probes to test the nucleotide sequence homology at these loci and the silent mating type genes HAIL and HMR. Restriction site polymorphisms were not detected inside the loci but in regions adjacent to them. These could be exploited in combination with tetrad analysis to identify four different chromosomes III in the distiller's strain. Of the four chromosomes, three were lacking HML and one was lacking HMR. The data are consistent with the assumption that all loci tested are organized in the same order as in the genetically analysed standard strains.

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Mutations affecting levels of tetrahydrofolate interconversion enzymes in Saccharomyces cerevisiae

Cited by 18 publications

References 20 publications

Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene.

Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene.

Genetic map of Saccharomyces cerevisiae.

Genetic and molecular characterization of a distiller’s yeast

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