Mutations in apoptotic genes and micronucleus occurrence in vinyl chloride‐exposed workers in China

Feng, Na; Zheng, Guoqiao; Hao, Yanhui; Li, Yong; Xu, Yue‐Ping; Xu, Xiaowen; Zhang, Guanghui; Wang, Jinwei; Li, Yongliang; Brandt‐Rauf, Paul W.; Zhao, Xin

doi:10.1002/em.22046

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Peripheral blood lymphocyte micronucleus formation rate is an indicator of the genetic damage level (Ghandhi et al, 2018). Many studies have shown that the peripheral blood lymphocyte micronucleus test can be used to assess the chromosomal damage of vinyl chloride in exposed workers and that the micronucleus rate can be used as an early-effect biomarker for vinyl chloride exposure (Feng et al, 2014(Feng et al, , 2017. In recent years, more and more studies confirmed that epigenetic alterations can occur independently or concomitantly with genotoxic aberrations and may directly influence the genotoxicity of chemical substances (Chappell et al, 2016;Virani et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Novel deoxyribonucleic acid methylation perturbations in workers exposed to vinyl chloride

et al. 2022

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To explore the epigenetic mechanism of deoxyribonucleic acid (DNA) damage induced by vinyl chloride (VC), we studied the micronuclei of peripheral blood lymphocytes in 193 subjects (92 in a VC exposure group employed in a chlorine-alkali plant; 101 in a control group employed in a power plant) and selected three pairs from the subjects (exposed and control) for whole-genome bisulfite sequencing (WGBS). The results showed that the rate of micronucleus formation in the VC exposure group was higher than that of control group (6.05 ± 3.28‰ vs. 2.01 ± 1.79‰). A total of 9534 differentially methylated regions (DMRs) were identified by WGBS, of which 4816 were hypomethylated and 4718 were hypermethylated. The Kyoto encyclopedia of genes and genomes (KEGG) pathway and gene ontology (GO) analyses showed the top three KEGG pathways were cancer , neuroactive ligand-receptor interaction, and axon guidance, and the top three GO-BP pathways enriched were multicellular organismal process, developmental process, and anatomical structure development. In the most enriched DMR pathway (pathways in cancer), we found that BCL2, TJP2, TAOK1, PFKFB3, LIPI, and LIPH were hypermethylated, and the methylation levels of BNIP1 and GRPEL2 were decreased. The methylation of differentially methylated genes (DMGs) mentioned above were verified by methylation-specific PCR (MSP) and agarose gel electrophoresis (AGE) in 50 pairs of subjects, where the coincidence rate was 60–100%. In conclusion, the epigenetic perturbations of specific DMGs ( BCL2, TJP2, TAOK1, PFKFB3, LIPI, LIPH, BNIP1, and GRPEL2) may be associated with DNA damage from vinyl chloride exposure.

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Section: Introductionmentioning

confidence: 99%

Novel deoxyribonucleic acid methylation perturbations in workers exposed to vinyl chloride

et al. 2022

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The lymphocyte cytokinesis block micronucleus test in human populations occupationally exposed to vinyl chloride: A systematic review and meta-analysis

Bolognesi

Bruzzone

Ceppi

et al. 2017

Mutation Research/Reviews in Mutation Research

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Micronucleus Induction by Exposure to Vinyl Chloride

Feng

Zhang

2019

The Micronucleus Assay in Toxicology

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Monomeric vinyl chloride is a colorless, with a slightly fragrant smell, gas at ordinary temperatures and pressures, which is widely used in the production of polyvinyl chloride, and is classified as a Group I human carcinogen by the International Agency for Research on Cancer (IARC) on the basis of increased risk for angiosarcoma of liver and hepatocellular carcinoma. The carcinogenicity of vinyl chloride could be mediated by a mutagenic mechanism. Following inhalation, vinyl chloride is rapidly absorbed and metabolized in the liver to the electrophilic metabolites chloroethylene oxide and chloroacetaldehyde, which could form DNA adducts that can be processed into point mutations in genes involved in pathways related to cancer in humans and rats exposed to vinyl chloride. The present chapter analyzes the published studies in which the cytokinesis-block micronucleus assay in peripheral lymphocytes was applied in vinyl chloride-exposed workers. Eight similar studies performed in China among three regions showed increased micronucleus frequencies (Frequency Ratio 1.92–3.79) associated with increased cumulative vinyl chloride exposure. The benchmark dose lower limit for 10% excess risk calculated from three studies resulted in an estimated exposure limit of 0.03–0.50 mg m−3. Furthermore, the results suggested it is very important to re-evaluate the recommended exposure limits using new bio-monitoring methods in addition to the micronucleus assay.

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Mutations in apoptotic genes and micronucleus occurrence in vinyl chloride‐exposed workers in China

Abstract: Genetic polymorphisms in genes related to apoptosis process may have an impact on chromosomal damage induced by vinyl chloride. Environ. Mol. Mutagen. 58:39-45, 2017. © 2016 Wiley Periodicals, Inc.

Cited by 3 publications

References 19 publications

Novel deoxyribonucleic acid methylation perturbations in workers exposed to vinyl chloride

Novel deoxyribonucleic acid methylation perturbations in workers exposed to vinyl chloride

The lymphocyte cytokinesis block micronucleus test in human populations occupationally exposed to vinyl chloride: A systematic review and meta-analysis

Micronucleus Induction by Exposure to Vinyl Chloride

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