Mutations in foot and mouth disease virus types A and O isolated from vaccinated animals

Abdulrahman, D A; El‐Deeb, Ayman H.; Shafik, Nermen Guda; Shaheen, Moahmed; Hussein, Heba A.

doi:10.20506/rst.38.3.3016

Cited by 5 publications

(4 citation statements)

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“…The six isolated FMD viruses and the two archived FMD viruses gave positive bands with the specific primer for serotype O, while no results with the specific primers for serotype A and SAT-2 as shown figure (2).…”

Section: Fmd Virus Serotyping Using Conventional Rt-pcrmentioning

confidence: 90%

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Isolation and Genetic characterization of foot and mouth disease virus causing an outbreak at Qalyabia government in 2021

Elbagory

El-Deen²,

Fakhry³

2022

Benha Veterinary Medical Journal

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Foot and mouth disease (FMD) is an extremely contagious transboundary disease that affects cloven-hoofed animals. An uncontrolled animal movement has a significant role in the disease circulation in endemic areas. An accurate and early diagnosis is critical for FMD control. Seventeen tongue epithelium and three vesicular fluid samples were collected from five cattle farms, and nine tongue epithelium and one vesicular fluid samples were collected from three buffalo farms suspected to be infected with FMDV at Qalyubia government in 2021. Trail of virus isolation was carried out on BHK-21 cell line followed by conventional RT-PCR for typing of the obtained virus isolate. Partial sequencing and phylogenetic analysis of VP1 for the field isolate revealed that it was serotype O topotype EA-3 lineage Alx-17 (FMDV/O/EGY/Qalyubia/2021/OM681353) and closely related to the previously local Egyptian isolate in 2017 (FMDV/O/EGY/2017/OM221230.1) with nucleotide identity 99.36% and sharing nucleotide similarity 99.72% with the previously isolated virus from Sudan (FMDV/O/SUD/2017/MK422569.1) and Ethiopia (FMDV/O/ETH/2017/ MN987453.1) during 2017 that suggested the transboundary incursion. The isolated FMDV/O/EA-3/Alx-17 shared 85.65% nucleotide identity with the sequenced FMDV serotype O topotype ME-SA lineage Sharqaui-72 (FMDV/O/EGY/OM681355), while 15.28% nucleotide difference between the isolated FMDV/O/EA-3/Alx-17 and the vaccine strain Pan-Asia-2 (FMDV/O/ME-SA/Pan-Asia-2/OK642671.1). The sequenced Sharqaui-72 virus revealed 86.01% sequence identity with the Pan-Asia-2 vaccine strain. The authors recommend for periodical molecular and genetic characterization between field isolates and vaccine strains, more cross-matching (R-value) and challenge studies between EA-3 viruses and PanAsia-2 vaccine strain, in addition to strict quarantine measures for the imported animals

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Section: Fmd Virus Serotyping Using Conventional Rt-pcrmentioning

confidence: 90%

“…The viral VP1-RNA was amplified by RT-PCR using specific primers for serotype O, A, and SAT2 as shown in table(2).…”

mentioning

confidence: 99%

Isolation and Genetic characterization of foot and mouth disease virus causing an outbreak at Qalyabia government in 2021

Elbagory

El-Deen²,

Fakhry³

2022

Benha Veterinary Medical Journal

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“…The relationship between the FMDV circulating strains in Egypt and vaccine strains (5,6,9,34,(38)(39)(40)(41). A-FMDV circulating strains within serotype A: (1-Topotype Africa G-VII and G-IV&2-Topotype Asia, lineage Iran-05).…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization and phylogenetic analysis of foot and mouth disease virus vaccine strains and recent field isolate

El-Bagoury¹,

El-Deen²,

Fakhry³

2023

IJVS

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Foot and Mouth Disease (FMD) control is a national and regional responsibility. FMD outbreaks were detected in three farms in Port-Said Governorate in 2020, although animals in these farms were vaccinated with the local polyvalent inactivated vaccine. Samples from tongue epithelium and vesicular fluid were collected from these farms, where FMDV isolation was performed on BHK-21 cell culture. Viral RNA was extracted from the virus isolates, and vaccine strains were then screened for FMDV using conventional Reverse transcriptase polymerase chain reaction. The substitution rates and phylogenetic relationship between the field isolate and the vaccine strains were determined using DNA sequence and bioinformatics analyses. The results illustrate that the sequence analysis for the 1D region of FMDV field isolate was serotype A-Africa topotype, Genotype IV, and closely related to isolated A-Africa topotype Genotype IV by Animal Health Research Institute, 2020 with nucleotide similarity ranging from 97.1% to 99.74%, and revealing genetic variation of 5.63-7.88% from previously Egyptian isolated A-Africa topotype, Genotype IV in 2016 and 2018. High genetic variations were determined from the locally used vaccine strain serotype A of the Asia topotype, lineage Iran-05, in the major antigenic sites of the VP1 region with a nucleotide difference of 26.34%. Depending on these findings, the Veterinary Serum and Vaccine Research Institute produced an emergency inactivated monovalent vaccine using the newly isolated strain, and we recommended adding it to the subsequent prepared vaccine batches.

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“…The high genetic and antigenic variability of FMDV and the existence of many lineages and sublineages are mainly due to error-prone RNA replication and extensive intra-and interserotype recombination [3,4]. Based on VP1 gene sequences, the serotypes are further classified into antigenically different topotypes [5]. Protective immune responses are elicited either by vaccination or by natural infection with a homologous strain [6].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the potency and effectiveness of a heptavalent oil-adjuvanted (ISA 206) foot-and-mouth disease vaccine in Egypt

et al. 2023

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Foot-and-mouth disease (FMD) is a serious highly contagious viral disease affecting all cloven-hoofed animals, and outbreaks can have a severe economic impact. An inactivated heptavalent oil-adjuvanted FMD vaccine (Aphtovac-7, MEVAC) was prepared from the foot-and-mouth disease virus (FMDV) strains A-Iran05, A-Africa-IV, O-PanAsia2, O-Manisa, O-EA3, SAT-2 Gharbia, and SAT-2 LIB-12. The vaccine potency and effectiveness were evaluated in three groups of 6- to 8-month-old calves and 200 adult dairy cattle under field conditions. All animals were vaccinated with the vaccine preparation, and the three groups of calves were challenged after 28 days by intradermolingual inoculation with 104 50% tissue culture infective dose (TCID50) of FMDV serotype A, O, or SAT-2. Mock-vaccinated calves (two per group) served as unvaccinated controls during the challenge test. Adult dairy cattle were tested for seroconversion using a virus neutralization test at 30, 60, and 120 days post-vaccination. All calves displayed complete protection against challenge with the different serotypes of FMDV when compared to the control groups. Serum samples collected after the primary and booster immunizations at 30 days post-vaccination contained high titers of protective antibodies (≥ 1/32; i.e. 1.5 log10). Antibodies persisted until the end of the study period (120 days), with a peak value around 60 days post-vaccination. The heptavalent FMD vaccine preparation was found to be potent and capable of providing a protective immune response under both experimental and field conditions.

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Mutations in foot and mouth disease virus types A and O isolated from vaccinated animals

Cited by 5 publications

References 0 publications

Isolation and Genetic characterization of foot and mouth disease virus causing an outbreak at Qalyabia government in 2021

Isolation and Genetic characterization of foot and mouth disease virus causing an outbreak at Qalyabia government in 2021

Genetic characterization and phylogenetic analysis of foot and mouth disease virus vaccine strains and recent field isolate

Assessment of the potency and effectiveness of a heptavalent oil-adjuvanted (ISA 206) foot-and-mouth disease vaccine in Egypt

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