MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

Abstract

A simple and label-free electrochemical aptasensor was developed for ultra-sensitive determination of chloramphenicol (CAP) based on a 2D transition of metal carbides (MXene) loaded with gold nanoparticles (AuNPs). The embedded AuNPs not only inhibit the aggregation of MXene sheets, but also improve the quantity of active sites and electronic conductivity. The aptamers (Apts) were able to immobilize on the MXene–AuNP modified electrode surface through Au–S interaction. Upon specifically binding with CAP with high affinity, the CAP–Apt complexes produced low conductivity on the aptasensor surface, leading to a decreased electrochemical signal. The resulting current change was quantitatively correlated with CAP concentration. Under optimized experimental conditions, the constructed aptasensor exhibited a good linear relationship within a wide range of 0.0001–10 nM and with a low detection limit of 0.03 pM for CAP. Moreover, the developed aptasensor has been applied to the determination of CAP concentration in honey samples with satisfactory results.

Recent trends in nanostructured carbon-based electrochemical sensors for the detection and remediation of persistent toxic substances in real-time analysis

“…In [Fe (CN) 6 ] 3− / 4− redox probe media, the CV arcs for several steps of altered electrodes were captured. The uncoated GCE electrode (slope a) displayed a distinct pair of reversible redox spikes, as seen in figure 8(A) [110]. Following immobilizing MXene-AuNPs, the reversible redox spike current significantly improved in comparison to naked GCE, and the peak potential separation (Ep) was reduced (curve b).…”

“…Focusing on MXene coated with Au NPs, Yang et al [110] created a straightforward and label-free electrochemical sensor (aptasensor) for excellent sensitivity measurement of chloramphenicol (CAP). Along with preventing MXene sheets from adhering together, the implanted AuNPs also increase the amount of active areas and electronic conductivity.…”

Bio-Tailored Sensing at the Nanoscale: Biochemical Aspects and Applications

“…These properties reflect the high number of similar devices which have been realized in recent years. Additional examples where the glassy carbon electrode is coated with hybrid nanostructured composites include: reduced MoS 2 -AuNPs for the double detection of zearalenone and fumonisin B1 mycotoxins in food [ 29 ]; (3-aminopropyl)triethoxysilane-modified graphene oxide (GO)-AgNPs for detection of chloramphenicol in food [ 30 ]; thiourea capped-ZnS quantum dots (QDs)-AuNPs for detection of β-casomorphin 7 in urine [ 31 ]; AgNPs-GQDs containing core-shell Cu-In-S/ZnSQDs for detection of ractopamine in urine and serum [ 32 ]; magnetic reduced GO-Fe 3 O 4 -Cu 2 O and Ag-resorcinol-formaldehyde NPs-Ag nanodots (NDs) for detection of prostate specific antigen in serum [ 33 ]; reduced GO-AgNPs and prussian blue-AuNPs for detection of acetamipridin in food [ 34 ]; reduced GO-AuNPs for detection of glycated albumin [ 35 ]; MXene-AuNPs for detection of chloramphenicol in food [ 36 ]; PtNPs-MIL-101(Fe) MOFs for detection of aflatoxin M1 in food [ 37 ]; and bimetallic CoNi-MOFs with graphene-like nanosheet structure to detect enrofloxacin in food, water and serum [ 38 ].…”

MXene-Based Nucleic Acid Biosensors for Agricultural and Food Systems

“…Chloramphenicol (CAP) is one of the common antibiotics banned from food production due to its potential for bone marrow aplasia, gray-baby syndrome, etc., but it still probably exists in foods of animal origin [ 118 ]. Yang et al [ 100 ] developed an electrochemical aptasensor using CAP aptamer immobilized on the MXene surface for the detection of CAP in honey as low as 0.03 pM. In another effort, Jiang et al [ 101 ] employed ZnO quantum dots/nitrogen (N)-doped MXene as a sensing framework that supported aptamers to interact with CAP specifically.…”