MYB Transcription Factors and a Putative Flavonoid Transporter ABCC-Like are Differentially Expressed in Radiata Pine Seedlings Exposed to Inclination

Gómez, Rocío Liliana; Gonzalez, Jaime; Herrera, Raúl; Ramos, Patricio

doi:10.1007/s00344-017-9707-5

Cited by 5 publications

(7 citation statements)

References 57 publications

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“…In our assay, NAC10 , NAC073 and MYB52 , MYB42 , MYB85 TFs were differentially expressed, and also showed relationships with other NACs and MYBs in the STRING network. Coincidently, several MYB TFs were characterized from radiata pine seedlings and are differentially-expressed on both sides of an inclined stem, yet preferentially on the upper side 85 . Whether NAC is modulating the expression of MYB and NAC genes, or if PrMADS10 could play a central role in this interaction in response to bending in pine, is an open question that requires further analysis.…”

Section: Discussionmentioning

confidence: 98%

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy

Cruz

Méndez

Ramos

et al. 2019

Sci Rep

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The molecular mechanisms underlying inclination responses in trees are unclear. In this study, we identified a MADS-box transcription factor differentially expressed early after inclination in the stems of Pinus radiata D. Don. PrMADS10 has a CDS of 582 bp and encodes a group II MADS-box transcription factor. We measured highest accumulation of this transcript on the lower side of inclined pine stems. In an effort to identify putative targets, we stably transformed Arabidopsis thaliana with a 35S::PrMADS10 construct. Transcriptome analysis revealed 1,219 genes differentially-expressed, with 690 and 529 genes up- and down-regulated respectively, when comparing the transgenic and wild-type. Differentially-expressed genes belong to different biological processes, but were enriched in cell wall remodeling and phenylpropanoid metabolic functions. Interestingly, lignin content was 30% higher in transgenic as compared to wild-type plants consistent with observed changes in gene expression. Differentially expressed transcription factors and phenylpropanoid genes were analyzed using STRING. Several MYB and NAC transcription factors showed interactions with genes of the phenylpropanoid pathway. Together, these results implicate PrMADS10 as a regulatory factor, triggering the expression of other transcription factors and genes involved in the synthesis of lignin.

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Section: Discussionmentioning

confidence: 98%

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy

Cruz

Méndez

Ramos

et al. 2019

Sci Rep

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“…Pinus radiata D. Don ABCC-flavonoid transporter (MRP1), MYB2, MYB5, MYB6, MYB10-flavonoid biosynthesis and homeostasis. [88] Pinus radiata D. Don MATE-flavonoid transporter (MATE1)-intracellular flavonoid homeostasis [89] Additionally, the expression pattern of genes associated with the phenylpropanoid biosynthetic pathway was studied. One study was performed to correlate the hormonal imbalance because of flavonoid differential accumulation with consequences for secondary cell wall modifications [32].…”

Section: Pinus Radiata D Donmentioning

confidence: 99%

“…[90,91]. Recently, the identification and transcriptional characterization of MYB TFs were assessed in radiata pine exposed to inclination stress [88]. Additionally, the promoter region of an ABCC transporter (PrMRP1) was isolated, and it contained putative cis-regulatory elements for MYBs and auxin-responsive elements [88].…”

Section: Pinus Radiata D Donmentioning

confidence: 99%

“…Regarding flavonoid homeostasis during the gravitropic response in wood-forming tissues of pine (see Table 3 ), putative transporters with high sequence identity to MATE (AtTT12) and ABCC (MRPs) were identified in SSH libraries [ 21 ]. These transporters were characterized in their expression during inclination stress and in response to hormones, showing robust auxin signaling dependence [ 88 , 89 ].…”

Section: Hormone and Flavonoid Metabolism During The Gravitropic Responsementioning

confidence: 99%

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A Talk between Flavonoids and Hormones to Reorient the Growth of Gymnosperms

Morales-Quintana

Ramos

2021

IJMS

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Plants reorient the growth of affected organs in response to the loss of gravity vector. In trees, this phenomenon has received special attention due to its importance for the forestry industry of conifer species. Sustainable management is a key factor in improving wood quality. It is of paramount importance to understand the molecular and genetic mechanisms underlying wood formation, together with the hormonal and environmental factors that affect wood formation and quality. Hormones are related to the modulation of vertical growth rectification. Many studies have resulted in a model that proposes differential growth in the stem due to unequal auxin and jasmonate allocation. Furthermore, many studies have suggested that in auxin distribution, flavonoids act as molecular controllers. It is well known that flavonoids affect auxin flux, and this is a new area of study to understand the intracellular concentrations and how these compounds can control the gravitropic response. In this review, we focused on different molecular aspects related to the hormonal role in flavonoid homeostasis and what has been done in conifer trees to identify molecular players that could take part during the gravitropic response and reduce low-quality wood formation.

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“…For example, antho-multidrug and toxic extrusion [antho-MATE1 (AM1)] and AM3 proteins mediate specifically acylated anthocyanin transport but could not transport cyanidin 3-O-glucoside or malvidin 3-O-glucoside, suggesting that the acyl conjugation was essential for the uptake 42 . Similar to many secondary metabolites, flavonoids are synthesized in the cytosol and transported into the vacuole to prevent cellular damage, where MATE and ATP-binding cassette (ABC) proteins are involved 44,45 . PDR-type ABC transporters have been reported in secretion of the flavonoid glycoside genistein from soybean roots 46 , and a multidrug resistance–associated protein (MRP), ZmMrp3, is required for anthocyanin transport in Zea mays 47 .…”

Section: Discussionmentioning

confidence: 99%

Widely targeted metabolome and transcriptome landscapes of Allium fistulosum–A. cepa chromosome addition lines revealed a flavonoid hot spot on chromosome 5A

Abdelrahman

Hirata²,

Sawada

et al. 2019

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Here, we report a comprehensive analysis of the widely targeted metabolome and transcriptome profiles of Allium fistulosum L. (FF) with the single extra chromosome of shallot [ A . cepa L. Aggregatum group (AA)] to clarify the novel gene functions in flavonoid biosynthesis. An exhaustive metabolome analysis was performed using the selected reaction monitoring mode of liquid chromatography–tandem quadrupole mass spectrometry, revealing a specific accumulation of quercetin, anthocyanin and flavone glucosides in AA and FF5A. The addition of chromosome 5A from the shallot to A . fistulosum induced flavonoid accumulation in the recipient species, which was associated with the upregulation of several genes including the dihydroflavonol 4-reductase , chalcone synthase , flavanone 3-hydroxylase , UDP-glucose flavonoid-3-O-glucosyltransferase , anthocyanin 5-aromatic acyltransferase-like , pleiotropic drug resistance-like ATP binding cassette transporter , and MYB14 transcriptional factor. Additionally, an open access Allium Transcript Database ( Allium TDB, http://alliumtdb.kazusa.or.jp ) was generated by using RNA-Seq data from different genetic stocks including the A . fistulosum–A . cepa monosomic addition lines. The functional genomic approach presented here provides an innovative means of targeting the gene responsible for flavonoid biosynthesis in A . cepa . The understanding of flavonoid compounds and biosynthesis-related genes would facilitate the development of noble Allium varieties with unique chemical constituents and, subsequently, improved plant stress tolerance and human health benefits.

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MYB Transcription Factors and a Putative Flavonoid Transporter ABCC-Like are Differentially Expressed in Radiata Pine Seedlings Exposed to Inclination

Cited by 5 publications

References 57 publications

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy

A Talk between Flavonoids and Hormones to Reorient the Growth of Gymnosperms

Widely targeted metabolome and transcriptome landscapes of Allium fistulosum–A. cepa chromosome addition lines revealed a flavonoid hot spot on chromosome 5A

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