Myelin basic protein: a multifunctional protein

Boggs, Joan M.

doi:10.1007/s00018-006-6094-7

Cited by 576 publications

(590 citation statements)

References 198 publications

(289 reference statements)

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“…This effect parallels the effects of RmAb on cultured oligodendrocytes and antiGalC on myelin (Dyer and Benjamins 1988;Bansal and Pfeiffer 1989;Menon et al 1997). Degradation of major MBP isoforms such as 18.5 and 17.5 kDa is important as 18.5 kDa isoform plays a major role in the compaction of myelin (Baumann and Pham-Dinh 2001;Harauz et al 2004;Boggs 2006) and 17.5 kDa is implicated in the formation of compact myelin (Kimura et al 1998). Thus a significant break down of MBP could lead to myelinolysis and thereby initiating the process of demyelination.…”

Section: Discussionmentioning

confidence: 54%

Diminished degradation of myelin basic protein by anti-sulfatide antibody and interferon-γ in myelin from glia maturation factor-deficient mice

Menon

Haas

et al. 2007

Neuroscience Research

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In this study we show the effect of anti-sulfatide (RmAb) antibodies and inflammatory cytokines, TNF-α and IFN-γ in inducing myelin basic protein (MBP) degradation in myelin isolated from control wild type (WT) and glia maturation factor (GMF)-deficient (GMF-KO) mice. GMF was not detected in isolated myelin from WT and GMF-KO mice although it is present in brains of WT mice. Our results show that calcium dependent neutral protease activity caused significantly elevated degradation of 18.5 and/or 17.5 kDa isoforms of MBP in WT-myelin treated with RmAb or IFN-γ. In contrast, MBP degradation in isolated myelin from GMF-KO mice remained unaffected following treatment with RmAb, IFN-γ, or GM-CSF. Neither the 14 kDa isoform of MBP or proteolipid protein (PLP) showed an elevated degradation compared to controls. A virtual absence of GM-CSF, TNF-α and IFN-γ in GMF-KO brain compared to WT was also evident when the animals were challenged with MOG 35-55. Additionally, the myelin from GMF-KO mice showed difference in distribution of myelin oligodendrocyte glycoprotein (MOG) and β-tubulin in a sucrose density gradient myelinaxolemmal fractions compared to WT. Taken together, our data suggests a role for GMF in the biochemical organization of myelin and thereby its effect on MBP degradation induced by RmAb and IFN-γ.

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Section: Discussionmentioning

confidence: 54%

Diminished degradation of myelin basic protein by anti-sulfatide antibody and interferon-γ in myelin from glia maturation factor-deficient mice

Menon

Haas

et al. 2007

Neuroscience Research

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“…Quantitative real time PCR (qRT‐PCR) was performed on cDNA synthesized from the CC and PFC RNA. The expression of four genes were investigated: GFAP, which has been used as an estimate of astrocyte activation and is considered a characteristic marker of brain injury;30 MBP , a myelin protein essential for normal myelination and axonal signal conduction31 which may be vulnerable to proteolysis following TBI;32 NFL, exclusive to neurons and involved in maintaining neuronal shape and function 33, 3435.…”

Section: Methodsmentioning

confidence: 99%

Sex matters: repetitive mild traumatic brain injury in adolescent rats

Wright

O’Brien

Shultz

et al. 2017

Ann Clin Transl Neurol

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ObjectiveWhether sex differences contribute to the heterogeneity of mild traumatic brain injury (mTBI) and repeated mTBI (RmTBI) outcomes in adolescents is unknown. Therefore, this study examined changes in, and differences between, male and female rats following single mTBI and RmTBI.MethodsRats were given a single mTBI, RmTBI (i.e., 3x), or sham injuries. Injuries were administered using a lateral impact model that mimics forces common in human mTBI. After the final injury, rats underwent extensive behavioral testing to examine cognition, motor function, and anxiety‐ and depressive‐like behavior. Postmortem analyses investigated gene expression and structural changes in the brain.ResultsMany of the outcomes exhibited a sex‐dependent response to RmTBI. While all rats given RmTBI had deficits in balance, motor coordination, locomotion, and anxiety‐like behavior, only male rats given RmTBI had short‐term working memory deficits, whereas only females given RmTBI had increased depressive‐like behavior. Volumetric and diffusion weighted MRI analyses found that while RmTBI‐induced atrophy of the prefrontal cortex was greater in female rats, only the male rats exhibited worse white matter integrity in the corpus callosum following RmTBI. Sex‐dependent changes in brain expression of mRNA for glial fibrillary acidic protein, myelin basic protein, and tau protein were also observed following injury.InterpretationThese findings suggest that in adolescent mTBI, sex matters; and future studies incorporating both male and females are warranted to provide a greater understanding of injury prognosis and better inform clinical practice.

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“…2−6 In myelin sheaths, the membrane becomes compact due to electrostatic interactions between the negatively charged lipids and positively charged myelin basic protein (MBP). 7,8 MBP, which represents about 10% of myelin by weight, is intrinsically disordered in solution. 9,10 When bound to a lipid surface, secondary structural elements form, and the hydrophobic parts of the protein are embedded in the bilayer.…”

Section: ■ Introductionmentioning

confidence: 99%

Structural Transition in Myelin Membrane as Initiator of Multiple Sclerosis

et al. 2016

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ABSTRACT:In demyelinating diseases such as multiple sclerosis, disrupted myelin structures impair the functional role of the sheath as an insulating layer for proper nerve conduction. Though the etiology and recovery pathways remain unclear, in vivo studies show alterations in the lipid and the adhesive protein (myelin basic protein, MBP) composition. We find that in vitro cytoplasmic myelin membranes with modified lipid composition and low MBP concentration, as in demyelinating disease, show structural instabilities and pathological phase transition from a lamellar to inverted hexagonal, which involve enhanced local curvature. Similar curvatures are also found in vivo in diseased myelin sheaths. In addition, MBP dimers form a correlated mesh-like network within the inner membrane space, only in the vicinity of native lipid composition. These findings delineate the distinct functional roles of dominant constituents in cytoplasmic myelin sheaths, and shed new light on mechanisms disrupting lipid−protein complexes in the diseased state.

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Myelin basic protein: a multifunctional protein

Cited by 576 publications

References 198 publications

Diminished degradation of myelin basic protein by anti-sulfatide antibody and interferon-γ in myelin from glia maturation factor-deficient mice

Diminished degradation of myelin basic protein by anti-sulfatide antibody and interferon-γ in myelin from glia maturation factor-deficient mice

Sex matters: repetitive mild traumatic brain injury in adolescent rats

Structural Transition in Myelin Membrane as Initiator of Multiple Sclerosis

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