Myogenic Differentiation of Stem Cells for Skeletal Muscle Regeneration

Dong-sheng, YU; Cai, Zijun; Li, Daishi; Zhang, Yi; He, Miao; Yang, Yuntao; Liu, Di; Xie, Wenqing; Li, Yusheng; Xiao, Wenfeng

doi:10.1155/2021/8884283

Cited by 43 publications

(26 citation statements)

References 105 publications

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“…Pax-7 functions upstream of myogenic factors, such as Myod and Myogenin. Myod promotes the development of myogenic precursors while Myogenin stimulates the differentiation of myoblast into myocytes and myotubes [ 61 ]. Repletion of 25(OH)D 3 significantly decreased mRNA expression of negative regulators of skeletal muscle mass (IL-1β, IL-6 and TNF-α as well as Atrogin-1, Murf-1 and Myostatin), while increasing the expression of positive regulators of skeletal muscle mass (Myod, Myogenin and Pax7) relative to repletion of 1,25(OH) 2 D 3 in CKD mice ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of 25-Hydroxyvitamin D3 versus 1α 25-Dihydroxyvitamin D3 on Adipose Tissue Browning in CKD-Associated Cachexia

Mak

Querfeld

Gonzalez

et al. 2021

Cells

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Patients with chronic kidney disease (CKD) often have low serum concentrations of 25(OH)D3 and 1,25(OH)2D3. We investigated the differential effects of 25(OH)D3 versus 1,25(OH)2D3 repletion in mice with surgically induced CKD. Intraperitoneal supplementation of 25(OH)D3 (75 μg/kg/day) or 1,25(OH)2D3 (60 ng/kg/day) for 6 weeks normalized serum 25(OH)D3 or 1,25(OH)2D3 concentrations in CKD mice, respectively. Repletion of 25(OH)D3 normalized appetite, significantly improved weight gain, increased fat and lean mass content and in vivo muscle function, as well as attenuated elevated resting metabolic rate relative to repletion of 1,25(OH)2D3 in CKD mice. Repletion of 25(OH)D3 in CKD mice attenuated adipose tissue browning as well as ameliorated perturbations of energy homeostasis in adipose tissue and skeletal muscle, whereas repletion of 1,25(OH)2D3 did not. Significant improvement of muscle fiber size and normalization of fat infiltration of gastrocnemius was apparent with repletion of 25(OH)D3 but not with 1,25(OH)2D3 in CKD mice. This was accompanied by attenuation of the aberrant gene expression of muscle mass regulatory signaling, molecular pathways related to muscle fibrosis as well as muscle expression profile associated with skeletal muscle wasting in CKD mice. Our findings provide evidence that repletion of 25(OH)D3 exerts metabolic advantages over repletion of 1,25(OH)2D3 by attenuating adipose tissue browning and muscle wasting in CKD mice.

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Section: Discussionmentioning

confidence: 99%

Differential Effects of 25-Hydroxyvitamin D3 versus 1α 25-Dihydroxyvitamin D3 on Adipose Tissue Browning in CKD-Associated Cachexia

Mak

Querfeld

Gonzalez

et al. 2021

Cells

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“…By means of histological analyses, the extra gains in muscle mass could be attributed to a larger increase in non-contractile tissue. Accordingly, mRNA levels of procollagen-1, IGF-1, and TGF-β were highly induced in IL-6-deficient mice, whereas the elevation of MyoD, required for myogenic differentiation ( Yu et al, 2021 ), was attenuated compared to WT animals. Collectively, these studies indicate that IL-6 is important for SC-mediated hypertrophy and may be involved in intramuscular connective tissue remodeling e.g., to prevent fibrosis in response to increased mechanical loading.…”

Section: Resistance Training and The Skeletal Muscle Secretomementioning

confidence: 98%

The Role of the Skeletal Muscle Secretome in Mediating Endurance and Resistance Training Adaptations

et al. 2021

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Exercise, in the form of endurance or resistance training, leads to specific molecular and cellular adaptions not only in skeletal muscles, but also in many other organs such as the brain, liver, fat or bone. In addition to direct effects of exercise on these organs, the production and release of a plethora of different signaling molecules from skeletal muscle are a centerpiece of systemic plasticity. Most studies have so far focused on the regulation and function of such myokines in acute exercise bouts. In contrast, the secretome of long-term training adaptation remains less well understood, and the contribution of non-myokine factors, including metabolites, enzymes, microRNAs or mitochondrial DNA transported in extracellular vesicles or by other means, is underappreciated. In this review, we therefore provide an overview on the current knowledge of endurance and resistance exercise-induced factors of the skeletal muscle secretome that mediate muscular and systemic adaptations to long-term training. Targeting these factors and leveraging their functions could not only have broad implications for athletic performance, but also for the prevention and therapy in diseased and elderly populations.

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“…Natural and synthetic materials, such as fibrin, alginate, polycaprolactone-based polymers and various other scaffold strategies, have been developed to generate Fig. 2 The graphical abstract of two therapy approaches based on stem cells for sarcopenia: TE and stem cell transplantation skeletal muscle tissues in vitro [90,91]. After stem cell isolation and purification to create an in vitro approximation of the in vivo stem cell niche and proliferation and differentiation in a simulated injury environment with proper cytokines, growth factors should be added to the blend of stem cells and scaffolds.…”

Section: Tementioning

confidence: 99%

The role and therapeutic potential of stem cells in skeletal muscle in sarcopenia

et al. 2022

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Sarcopenia is a common age-related skeletal muscle disorder featuring the loss of muscle mass and function. In regard to tissue repair in the human body, scientists always consider the use of stem cells. In skeletal muscle, satellite cells (SCs) are adult stem cells that maintain tissue homeostasis and repair damaged regions after injury to preserve skeletal muscle integrity. Muscle-derived stem cells (MDSCs) and SCs are the two most commonly studied stem cell populations from skeletal muscle. To date, considerable progress has been achieved in understanding the complex associations between stem cells in muscle and the occurrence and treatment of sarcopenia. In this review, we first give brief introductions to sarcopenia, SCs and MDSCs. Then, we attempt to untangle the differences and connections between these two types of stem cells and further elaborate on the interactions between sarcopenia and stem cells. Finally, our perspectives on the possible application of stem cells for the treatment of sarcopenia in future are presented. Several studies emerging in recent years have shown that changes in the number and function of stem cells can trigger sarcopenia, which in turn leads to adverse influences on stem cells because of the altered internal environment in muscle. A better understanding of the role of stem cells in muscle, especially SCs and MDSCs, in sarcopenia will facilitate the realization of novel therapy approaches based on stem cells to combat sarcopenia.

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Myogenic Differentiation of Stem Cells for Skeletal Muscle Regeneration

Cited by 43 publications

References 105 publications

Differential Effects of 25-Hydroxyvitamin D3 versus 1α 25-Dihydroxyvitamin D3 on Adipose Tissue Browning in CKD-Associated Cachexia

Differential Effects of 25-Hydroxyvitamin D3 versus 1α 25-Dihydroxyvitamin D3 on Adipose Tissue Browning in CKD-Associated Cachexia

The Role of the Skeletal Muscle Secretome in Mediating Endurance and Resistance Training Adaptations

The role and therapeutic potential of stem cells in skeletal muscle in sarcopenia

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