Myroides marinus sp. nov., a member of the family Flavobacteriaceae, isolated from seawater

et al., 1979;Macfarlane et al., 1985;Ferrer et al., 1995;Hsueh et al., 1995;Green et al., 2001; Yagcı et al., 2000), recent studies have indicated that they are also common inhabitants of soil and water and behave as low-grade opportunistic pathogens (Hugo et al., 2006). A novel bacterial strain, designated MY15 T , was isolated from a saliva sample obtained from an asymptomatic student during a teaching experiment at Weifang Medical University, China. A further attempt to isolate the bacterium from the same student a week later failed. The aim of the present study was to determine the exact taxonomic position of strain MY15T by using a polyphasic taxonomic approach.The saliva sample was diluted with sterile saline (0.9 % NaCl; w/v) and spread onto trypticase soy agar (TSA; Difco) plates that were incubated at 37 u C for 48 h. Strain MY15 T , which produced a yellow to brown diffusible pigment, was purified by streaking three times on TSA. Cultures were maintained on Luria-Bertani (LB) agar For 16S rRNA gene sequencing, cells grown in LB broth at 28 u C for 24 h were harvested by centrifugation. Extraction and purification of total genomic DNA from the cells and PCR amplification of the 16S rRNA gene were performed as described by Jin et al. (2010). The nearly complete 16S rRNA gene sequence (1356 nt) of strain MY15T was submitted to GenBank and EMBL to search for similar sequences using the BLAST algorithm. The identification of phylogenetic neighbours and the calculation of pairwise 16S rRNA gene sequence similarities were achieved using the EzTaxon server (http://www.eztaxon.org/; Chun et al., 2007). Multiple sequence alignment was performed by using CLUSTAL_X 1.8The GenBank/EMBL/DDBJ accession number for 16S rRNA gene sequence of Myroides phaeus sp. nov. MY15T is GU253339.A supplementary figure is available with the online version of this paper.

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“…This value falls within the range (33.8-36.7 %) reported for strains of the genus Myroides (Cho et al, 2011).…”

Section: Strain My15supporting

confidence: 86%

Myroides phaeus sp. nov., isolated from human saliva, and emended descriptions of the genus Myroides and the species Myroides profundi Zhang et al. 2009 and Myroides marinus Cho et al. 2011

Yan

Zhao

Zhang

2012

International Journal of Systematic and Evolutionary Microbiology

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“…In recent years, three novel species (Myroides pelagicus, Myroides profundi and Myroides marinus) were isolated from seawater (Yoon et al, 2006;Zhang et al, 2008;Cho et al, 2011). Although commonly found in soil and water, Myroides spp.…”

Section: Discussionmentioning

confidence: 99%

Septicaemia caused by Myroides spp.: a case report

et al. 2015

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Introduction: Myroides spp. are aerobic, yellow-pigmented, non-fermentative Gram-negative rods considered to be low-grade opportunistic pathogens. However, their intrinsic resistance to commonly used antibiotics necessitates prompt identification and evaluation. Case presentation: Here, we report a case of septicaemia caused by Myroides sp. A 36-year-old pregnant female was admitted to the intensive care unit at week 18 of gestation with a diagnosis of hydatidiform mole with acute renal failure and anaemia. Blood culture under aerobic conditions showed growth of round, smooth, convex, yellow-pigmented colonies on blood agar but which failed to grow on MacConkey agar, and the organism was identified as Myroides sp. Susceptibility testing showed that it was susceptible only to piperacillin+tazobactam and, following treatment, the patient recovered. Conclusion: Myroides spp. has, until now, been regarded as an opportunistic pathogen and a rare cause of serious disease in immunocompromised subjects. However, it may cause serious disease, even in immunocompetent hosts, and its intrinsic resistance warrants appropriate testing and timely intervention.

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“…nov. (Zhang et al, 2014)), seawater (strain JS-08(T), named M. marinus sp. nov. (Cho et al, 2011), strain SM1(T), named M. pelagicus sp. nov. (Yoon et al, 2006)), deep-sea sediment (strain D25T (Zhang et al, 2008)), human saliva (strain MY15T, named M. phaeus sp.…”

mentioning

confidence: 99%

Antibiotic resistance mechanisms of Myroides sp.

Yuan

et al. 2016

J. Zhejiang Univ. Sci. B

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Bacteria of the genus Myroides (Myroides spp.) are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA (rRNA) gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that obtaining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infections. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.

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Myroides marinus sp. nov., a member of the family Flavobacteriaceae, isolated from seawater

Cited by 34 publications

References 20 publications

Myroides phaeus sp. nov., isolated from human saliva, and emended descriptions of the genus Myroides and the species Myroides profundi Zhang et al. 2009 and Myroides marinus Cho et al. 2011

Myroides phaeus sp. nov., isolated from human saliva, and emended descriptions of the genus Myroides and the species Myroides profundi Zhang et al. 2009 and Myroides marinus Cho et al. 2011

Septicaemia caused by Myroides spp.: a case report

Antibiotic resistance mechanisms of Myroides sp.

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