N-alkylimidazole derivatives as potential inhibitors of quorum sensing in Pseudomonas aeruginosa

De novo peptides as potential antimicrobial agents

“…Serial dilutions of the peptides (1.878 mM–917.5 nM) were made in triplicate in microtiter plates and then 100 μL of the broth containing the bacteria was transferred into each well of the microtiter plate to make a total volume of 200 μL. The plates were incubated at 37 °C for 24 h. The MICs were determined as the lowest peptide concentration that inhibited bacterial growth using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) indicator [ [29] , [30] , [31] ].…”