NADPH oxidases: an overview from structure to innate immunity-associated pathologies

Panday, Arvind; Sahoo, Malaya K.; Osorio, Diana; Batra, Sanjay

doi:10.1038/cmi.2014.89

Cited by 804 publications

(713 citation statements)

References 291 publications

(274 reference statements)

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“…The NADPH oxidase complex catalyzes electron transfer from NADPH to molecular oxygen and thus generates superoxide, the precursor of H 2 O 2 and other ROS (29,46). Genetic defects in NADPH oxidase cause chronic granulomatous disease, an inherited primary immunodeficiency associated with autoinflammation and autoimmunity (47)(48)(49).…”

Section: Methodsmentioning

confidence: 99%

“…To test whether CD8 Treg deficiency is a feature of all inflammatory diseases, we recruited middle-aged and older patients with psoriatic arthritis (PsA), an aggressive inflammatory syndrome of the skin and the joints (28). Middleaged patients with PsA were able to generate NOX2-expressing CD8 Tregs, and patients with PsA over 60 years of age were indistinguishable from healthy older individuals (Figure 4, C-E (29,30), but it also mediates tissue damage by ROS production. When loaded with oxidation-sensitive fluorogenic probes, CD8 Tregs and nonsuppressive, freshly isolated CD8 T cells could be clearly distinguished in that only CD8 Tregs were able to produce high levels of intracellular ROS ( Figure 5, A and B).…”

Section: Cd26mentioning

confidence: 99%

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NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs

Wen

Shimojima

Shirai

et al. 2016

Journal of Clinical Investigation

118

110

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Section: Methodsmentioning

confidence: 99%

Section: Cd26mentioning

confidence: 99%

NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs

Wen

Shimojima

Shirai

et al. 2016

Journal of Clinical Investigation

118

110

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“…Allergen exposure drives oxidative stress by causing airway epithelium and smooth muscle cells to produce ROS, particularly O 2 -, through activation of NADPH oxidase (4,5). Other sources of ROS include inflammatory cells like eosinophils (6), neutrophils (7), and monocytes/macrophages (8).…”

Section: Introductionmentioning

confidence: 99%

The R213G polymorphism in SOD3 protects against allergic airway inflammation

Gaurav¹,

Varasteh²,

Weaver³

et al. 2017

JCI Insight

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“…ROS production may increase through modulators of its expression, such as transforming growth factor β, angiotensin II, tumor necrosis factor alpha (TNF-α), and shear stress. A decrease in expression is observed in the presence of platelet-derived growth factor (PDGF), hypoxia-induced factor 1 (HIF-1), and peroxisome proliferator-activated receptor (PPAR) gamma 14,15 . Our results showed that the Nox4 gene responded with hyperexpression during IR (IRG), which was characteristic of oxidative stress ( Table 2).…”

Section: Nadph Oxidase 4 (Nox4) Genementioning

confidence: 99%

“…This protein has a high expression level in cardiac tissue, although the degree of the difference in expression between the vessels and the myocardium is unclear. NOX4 presents a differentiated pattern of ROS production following binding of NADPH to its terminal carbon, which facilitates electron transfer, and thereafter via binding of the flavin adenine dinucleotide (FAD) to two heme residues and then to molecular oxygen; this process predominately acts as a source of hydrogen peroxide (H 2 O 2 ) and, to a lesser extent, as a source of superoxide anion (O 2 -) 13,14 . ROS production may increase through modulators of its expression, such as transforming growth factor β, angiotensin II, tumor necrosis factor alpha (TNF-α), and shear stress.…”

Section: Nadph Oxidase 4 (Nox4) Genementioning

confidence: 99%

Hyperbaric oxygenation and the genic expression related to oxidative stress in the heart of mice during intestinal ischemia and reperfusion

Neto

Ikejiri²,

Bertoletto³

et al. 2017

Acta Cir. Bras.

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Hyperbaric oxygenation and the genic expression related to oxidative stress in the heart of mice during intestinal ischemia and reperfusion 1 3-Experimental SurgeryActa Cir Bras. 2017;32(11):913-923 AbstractPurpose: To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. Methods: Four groups were subjected to 60 minutes of intestinal ischemia followed by 60 minutes of reperfusion: IRG, ischemia and reperfusion group without HBO; HBO-IG, which received HBO during ischemia; HBO-RG, which received HBO during reperfusion; and HBO-IRG, which received HBO during ischemia and reperfusion. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes. The (RT-qPCR) method was applied. Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student's t-test p<0.05).Results: Eight genes (9.52%) were hyperexpressed in the IRG. When the HBO groups were compared to the IRG, we found a decrease in the expression of eight genes in the HBO-IG, five genes in the HBO-RG, and seven genes in the HBO-IRG. Conclusion:The reduction in the expression of genes related to oxidative stress and antioxidant defense following HBO in mouse hearts resulting from intestinal IR injury was more favorable during the ischemic period than during the reperfusion period. Thirty male isogenic mice (C57BL/6) from the Center for the Development of Experimental Models in Medicine and Biology (CEDEME -UNIFESP) were housed under controlled temperature and light conditions with a 12:12 hour light: dark cycle and free access to water and pelleted rations for up to 6 hours before the surgical procedures. Anesthetic and surgical proceduresThe mice were anesthetized intramuscularly with 44 mg.kg -1 of ketamine hydrochloride solution (Ketamina AgenerUnião Química, São Paulo), 2.5 mg.kg -1 of xylazine hydrochloride (Calmium -União Química, São Paulo), and 0.75 mg.kg -1 of acepromazine (Acepran -Rhobifarma, Hortolândia-SP). The control group (CG) was anesthetized, subjected to laparotomy without occlusion of the superior mesenteric vessels, and observed for 120 minutes. The IR group (IRG) was subjected to 60 minutes of superior mesenteric artery occlusion, followed by 60 minutes of reperfusion. Three groups received HBO as follows: during ischemia (HBO-IG), during reperfusion (HBO-RG), and during ischemia and during reperfusion (HBO-IRG). Material collection procedureSamples were taken from the small intestine and the heart. The fragments of the hearts and the intestinal segments were washed with saline solution, wrapped in labeled aluminum foil, immersed in liquid nitrogen (-196°C), and sent to the Laboratory ■ IntroductionIschemia-reperfusion (IR) injuries with cellular alterations and humoral disorders lead to an imbalance in homeostasis 1 . These injuries occur as a resu...

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NADPH oxidases: an overview from structure to innate immunity-associated pathologies

Cited by 804 publications

References 291 publications

NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs

NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs

The R213G polymorphism in SOD3 protects against allergic airway inflammation

Hyperbaric oxygenation and the genic expression related to oxidative stress in the heart of mice during intestinal ischemia and reperfusion

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