Nano‐Structured Cell‐Adhesive and Cell‐Repulsive Plasma‐Deposited Coatings: Chemical and Topographical Effects on Keratinocyte Adhesion

Abstract

Cell‐adhesive and cell‐repulsive coatings have been plasma‐deposited on poly(ethylene terephthalate) surfaces previously structured with nano‐metric conical features by means of colloidal lithography. Surface analysis revealed that both coatings are conformal on nano‐structured substrates, with their wettability depending on the substrate morphology. The effect of surface chemistry and surface topography on cell adhesion has been investigated and clarified. The adhesion of a human keratinocyte cell‐line was found to be strongly dependent on the surface topography for plasma‐deposited acrylic acid (cell‐adhesive), and on the surface chemistry for poly(ethylene oxide)‐like (cell‐repulsive) coatings.

Controlling roughness: from etching to nanotexturing and plasma-directed organization on organic and inorganic materials

“…It should also be noted that nanotexturing permits control of cell growth on the surface as recently discussed in several reports (64,65) …”

Surface engineering of synthetic polymer materials for tissue engineering and regenerative medicine applications

“…[37][38][39][40][41][42] Furthermore, the effect of orthogonal-gradients of chemical and physical modifications was studied to elucidate the combinatorial effects of both attributes on cell behaviour. 43 Although in most cases a sequential strategy have been taken to impose both modifications onto surface, more recently new methods has been developed to impose both modifications in one step such as reactive imprint lithography (RIL).…”

Investigation of air-DBD effects on biological liquids for in vitro studies on eukaryotic cells

“…Low pressure (LP) plasmas are still mostly utilized in this highly interdisciplinary field, for producing cell-adhesive layers on flat or within porous substrates, functionalized surfaces decorated with biomolecules, non fouling coatings, anti-bacterial surfaces and films with hydrogel properties just to mention a few examples [1][2][3][4][5][6][7][8][9]. AP plasmas are now also utilized for surface modification of biomedical materials as well as for sterilization [10][11][12].…”