Nanocarrier-Assisted Antimicrobial Therapy Against Intracellular Pathogens

Kumar, Lalit; Verma, Shivani; Vaidya, Bhuvaneshwar; Mehra, Neelesh Kumar

doi:10.1016/b978-0-323-46152-8.00013-5

Cited by 9 publications

(8 citation statements)

References 165 publications

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“…Fluoroquinolones and macrolides diffuse well into cells but have low intracellular retention time. 10 Moreover, the efficiency of antibiotics would be decreased by the intracellular pH and enzymes. 11,12 Nanocarriers can improve the penetration and stability of antibiotics via targeted delivery to efficiently kill the intracellular bacteria.…”

Section: Introductionmentioning

confidence: 99%

Macrophage-targeting bioactive glass nanoparticles for the treatment of intracellular infection and subcutaneous abscess

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Macrophage-targeting bioactive glass nanoparticles for the treatment of intracellular infection and subcutaneous abscess

et al. 2022

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“…In general, larger rigid particles over 2 μm in diameter can accumulate in the spleen, liver, and the lung capillaries . For example, following intravenous (IV) administration, MPs larger than 7 μm in size have been reported to be captured by lung capillaries, enabling localized lung delivery . In a study of oral drug delivery formulations for intestinal administration, PLGA MPs with a diameter ranging from 1 to 10 μm showed lower cell uptake compared to 100 nm nanoparticles (NPs) …”

Section: Micro- and Nanoparticle-based Peptide Delivery Formulationsmentioning

confidence: 99%

Recent Advances in Formulations for Long-Acting Delivery of Therapeutic Peptides

Zangabad

Rezvani

Tong

et al. 2023

ACS Appl. Bio Mater.

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Recent preclinical and clinical studies have focused on the active area of therapeutic peptides due to their high potency, selectivity, and specificity in treating a broad range of diseases. However, therapeutic peptides suffer from multiple disadvantages, such as limited oral bioavailability, short half-life, rapid clearance from the body, and susceptibility to physiological conditions (e.g., acidic pH and enzymolysis). Therefore, high peptide dosages and dose frequencies are required for effective patient treatment. Recent innovations in pharmaceutical formulations have substantially improved therapeutic peptide administration by providing the following advantages: long-acting delivery, precise dose administration, retention of biological activity, and improvement of patient compliance. This review discusses therapeutic peptides and challenges in their delivery and explores recent peptide delivery formulations, including micro/nanoparticles (based on lipids, polymers, porous silicon, silica, and stimuli-responsive materials), (stimuli-responsive) hydrogels, particle/hydrogel composites, and (natural or synthetic) scaffolds. This review further covers the applications of these formulations for prolonged delivery and sustained release of therapeutic peptides and their impact on peptide bioactivity, loading efficiency, and (in vitro/in vivo) release parameters.

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“…Therefore, for the efficient killing of such intracellular microorganisms, antimicrobials must reach phagosomes or even phagolysosomes. Polymeric particles (PPs) are promising carrier systems for the delivery of antimicrobial drugs to immune cells (Donnellan and Giardiello 2019 ; Kumar et al 2017 ; Lee et al 2015 ; Mejía et al 2021 ) and potentially to phagosomes. However, before such drug delivery systems can be further developed, it is necessary to assess whether a PP can reach a phagolysosome containing a pathogenic microorganism.…”

Section: Introductionmentioning

confidence: 99%

Targeting of phagolysosomes containing conidia of the fungus Aspergillus fumigatus with polymeric particles

González

Gangapurwala

Alex

et al. 2022

Appl Microbiol Biotechnol

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Conidia of the airborne human-pathogenic fungus Aspergillus fumigatus are inhaled by humans. In the lung, they are phagocytosed by alveolar macrophages and intracellularly processed. In macrophages, however, conidia can interfere with the maturation of phagolysosomes to avoid their elimination. To investigate whether polymeric particles (PPs) can reach this intracellular pathogen in macrophages, we formulated dye-labeled PPs with a size allowing for their phagocytosis. PPs were efficiently taken up by RAW 264.7 macrophages and were found in phagolysosomes. When macrophages were infected with conidia prior to the addition of PPs, we found that they co-localized in the same phagolysosomes. Mechanistically, the fusion of phagolysosomes containing PPs with phagolysosomes containing conidia was observed. Increasing concentrations of PPs increased fusion events, resulting in 14% of phagolysosomes containing both conidia and PPs. We demonstrate that PPs can reach conidia-containing phagolysosomes, making these particles a promising carrier system for antimicrobial drugs to target intracellular pathogens. Key points • Polymer particles of a size larger than 500 nm are internalized by macrophages and localized in phagolysosomes. • These particles can be delivered to Aspergillus fumigatus conidia-containing phagolysosomes of macrophages. • Enhanced phagolysosome fusion by the use of vacuolin1 can increase particle delivery.

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Nanocarrier-Assisted Antimicrobial Therapy Against Intracellular Pathogens

Cited by 9 publications

References 165 publications

Macrophage-targeting bioactive glass nanoparticles for the treatment of intracellular infection and subcutaneous abscess

Macrophage-targeting bioactive glass nanoparticles for the treatment of intracellular infection and subcutaneous abscess

Recent Advances in Formulations for Long-Acting Delivery of Therapeutic Peptides

Targeting of phagolysosomes containing conidia of the fungus Aspergillus fumigatus with polymeric particles

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