Nanoceria-mediated delivery of doxorubicin enhances the anti-tumour efficiency in ovarian cancer cells via apoptosis

Das, Joydeep; Choi, Yun‐Jung; Han, Jung Soo; Reza, Abu Musa Md Talimur; Kim, Jin‐Hoi

doi:10.1038/s41598-017-09876-w

Cited by 84 publications

(35 citation statements)

References 49 publications

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“…Furthermore, NC enhances the antitumor activity of doxorubicin and increases apoptosis in cancer cells. NC also has cytotoxic effects; however, cotreatment of cancer cells with doxorubicin and NC showed synergistic effects (34). In our pilot study, we observed the cytotoxic effect of NC at a dose of 5 mg/kg, and co-treatment of NC and CP markedly decreased oxidative stress marker levels, liver injury enzyme levels, histopathological changes, and apoptosis in the liver (not published).…”

Section: Discussionmentioning

confidence: 84%

Anti-apoptotic and Antioxidant Effect of Cerium Oxide Nanoparticles on Cyclophosphamide-Induced Hepatotoxicity

Amiri¹,

Hamzeh²,

Beklar³

et al. 2018

Erciyes Med J

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Objective: Cyclophosphamide (CP), as an anticancer agent has side effects, especially hepatotoxicity. Cerium oxide nanoparticles [nanoceria (NC)], which act as antioxidants, have a protective effect against oxidative stress-induced toxicity. This study evaluated the preventive effect of NC in hepatotoxicity induced by CP. Materials and Methods: Thirty-two mice were randomly distributed into four groups: control (received only normal saline), NC (received NC 100 μg/kg for 3 days, intraperitoneally), CP (received CP 200 mg/kg single dose on the third day of the study, intraperitoneally), and NC+CP (NC 100 μg/kg and CP 200 mg/kg). Two days after the final treatment, oxidative stress marker (MDA and GSH) and alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels were measured, and histopathological and immunohistochemical examinations were performed for determining the effects of NC on hepatotoxicity. Results: CP-induced hepatotoxicity was evident by the significant elevation of liver enzyme levels and alteration in oxidative stress marker levels. Additionally, histopathological changes and apoptosis were markedly increased. NC decreased MDA level, increased GSH level and decreased liver enzyme levels in CP-treated mice. In addition, NC pretreatment could be alleviated by the immunoreactivity of caspase-3 in CP-treated mice compared with the CP group. Conclusion: NC showed a potent antioxidant effect in hepatotoxicity induced by CP. The study suggested that NC through its antioxidant and anti-apoptotic properties has a protective effect against hepatotoxicity induced by CP.

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Section: Discussionmentioning

confidence: 84%

Anti-apoptotic and Antioxidant Effect of Cerium Oxide Nanoparticles on Cyclophosphamide-Induced Hepatotoxicity

Amiri¹,

Hamzeh²,

Beklar³

et al. 2018

Erciyes Med J

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“…Also, they were exhibited that NC have cytotoxic effects. But co-treatment cancer cells with doxorubicin and NC showed synergistic effects [49]. In our pilot study, we saw cytotoxic effect of NC in dose of 5 mg/kg for 7 days, and co-treatment NC and CP markley decreased oxidative stress markers, renal injury markers, histopathological and apoptosis in kidney [not publish].…”

Section: Discussionmentioning

confidence: 99%

Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via anti-apoptotic and antioxidant properties in BALB/c mice

Hamzeh¹,

Amiri²,

Beklar³

et al. 2018

mpj

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Cyclophosphamide [CP], as alkylating agents has side effects such as nephrotoxicity. Cerium oxide nanoparticles [nanoceria; NC], as an antioxidant, are effective at reduction oxidative stress. This study evaluated the protective effect of nanoceria in nephrotoxicity induced CP. 32 BALB/c mice were randomly divided into four equal groups. Control, NC, CP and NC+CP. NC and CP injected intraperitoneally respectively in dose of 100 μg/kg for 3 days and 200 mg/kg single dose on 3th day of study. Two days after the final treatment, histochemical, serum biochemical, histopathological and immunohistochemical examinations were performed for determination effects of NC on nephrotoxicity. Oxidative stress and renal injury induced in CP treated mice were proved by the significantly elevation of urea and creatinine and alteration in oxidative stress markers [MDA and GSH levels]. Consequently, histopathological changes and apoptosis were markedly increased. NC was able to reduce MDA, urea , creatinine and increase GSH content. In addition, NC pretreatment could alleviated immunoreactivity of caspase-3. NC revealed a strong antioxidant in nephrotoxicity following CP treatment. This study suggests that NC through antioxidant and antiapoptotic properties have protective effect against CP-induced nephrotoxicity.

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“…In 2017, Das et al [ 116 ] loaded doxorubicin (a chemotherapeutic drug) on nanoceria by electrostatic interactions between negatively charged nanoceria and positively charged doxorubicin at pH 7.0.…”

Section: Synthesis Of Nanoceriamentioning

confidence: 99%

“… Chlorin e6 Human breast cancer cells (MCF-7/ADR) and MCF-7/ADR xenograft murine model Showed photodynamic therapy in drug-resistant breast cancer cells and tumor in vivo [ 148 ] 4. Doxorubicin Human Ovarian cancer cell lines A2780, SKOV-3, and CAOV-3 Higher cell proliferation inhibition and apoptosis compared with free DOX [ 116 ] 5. Doxorubicin and Hsp90 inhibitor ganetespib (GT) A549 cells Co-delivery of Dox and GT using Nanoceria showed more than 80% of NSCLC death within 48 h of incubation [ 33 ] 6.…”

Section: Biomedical Applications Of Nanoceriamentioning

confidence: 99%

“…In 2017, Das et al [ 116 ] reported nanoceria-mediated drug delivery of doxorubicin in human ovarian cancer cells. They found that doxorubicin-loaded nanoceria (CeO 2 /DOX) possessed excellent drug-loading content (22.41%) and drug release behavior at the acidic and high reducing environment as well as increased cellular uptake and retention of the drug as compared to free doxorubicin.…”

Section: Biomedical Applications Of Nanoceriamentioning

confidence: 99%

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Synthesis and biomedical applications of nanoceria, a redox active nanoparticle

2019

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Background Nanoceria has recently received much attention, because of its widespread biomedical applications, including antibacterial, antioxidant and anticancer activity, drug/gene delivery systems, anti-diabetic property, and tissue engineering. Main body Nanoceria exhibits excellent antibacterial activity against both Gram-positive and Gram-negative bacteria via the generation of reactive oxygen species (ROS). In healthy cells, it acts as an antioxidant by scavenging ROS (at physiological pH). Thus, it protects them, while in cancer cells (under low pH environment) it acts as pro-oxidant by generating ROS and kills them. Nanoceria has also been effectively used as a carrier for targeted drug and gene delivery in vitro and in vivo models. Besides, nanoceria can also act as an antidiabetic agent and confer protection towards diabetes-associated organ pathophysiology via decreasing the ROS level in diabetic subjects. Nanoceria also possesses excellent potential in the field of tissue engineering. In this review, firstly, we have discussed the different methods used for the synthesis of nanoceria as these are very important to control the size, shape and Ce 3+ /Ce 4+ ratio of the particles upon which the physical, chemical, and biological properties depend. Secondly, we have extensively reviewed the different biomedical applications of nanoceria with probable mechanisms based on the literature reports. Conclusion The outcome of this review will improve the understanding about the different synthetic procedures and biomedical applications of nanoceria, which should, in turn, lead to the design of novel clinical interventions associated with various health disorders. Graphical abstract

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Nanoceria-mediated delivery of doxorubicin enhances the anti-tumour efficiency in ovarian cancer cells via apoptosis

Cited by 84 publications

References 49 publications

Anti-apoptotic and Antioxidant Effect of Cerium Oxide Nanoparticles on Cyclophosphamide-Induced Hepatotoxicity

Anti-apoptotic and Antioxidant Effect of Cerium Oxide Nanoparticles on Cyclophosphamide-Induced Hepatotoxicity

Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via anti-apoptotic and antioxidant properties in BALB/c mice

Synthesis and biomedical applications of nanoceria, a redox active nanoparticle

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