Nanocomplexes of carboxymethyl chitosan/amorphous calcium phosphate reduce oral bacteria adherence and biofilm formation on human enamel surface

He, Jiankang; Bao, Yi-Jun; Li, Jie; Qiu, Zhongjun; Liu, Ying; Zhang, Xu

doi:10.1016/j.jdent.2018.11.003

Cited by 34 publications

(22 citation statements)

References 52 publications

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“…In the CMC-ClyR-ACP nanogel, CMC could still maintain calcium and phosphorus at an amorphous state. This is because CMC, like known existing stabilizers of ACP [36,37], has abundant carboxymethyl groups that protect calcium ions on the inner side of the particles from negatively charged phosphate ions [27][28][29]38,39].…”

Section: Discussionmentioning

confidence: 99%

The dual anti‐caries effect of carboxymethyl chitosan nanogel loaded with chimeric lysin ClyR and amorphous calcium phosphate

Zhu

Yan

Mujtaba

et al. 2021

European J Oral Sciences

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In this study, we evaluated the anti-biofilm and anti-demineralization abilities of a novel material, CMC-ClyR-ACP nanogel, designed by loading the chimeric lysin ClyR and amorphous calcium phosphate (ACP) into a nanocarrier material carboxymethyl chitosan (CMC), in a demineralization model. Dynamic light scattering, transmission electron microscopy, and Fourier transmission infrared spectroscopy showed that CMC-ClyR-ACP nanogel was synthesized successfully. Enamel samples prepared from premolars were divided into five groups according to their treatments with: (i) double distilled water ddH 2 O, (ii) CMC-ACP, (iii) CMC-ClyR-ACP, (iv) ClyR, or (v) 0.12% chlorhexidine. Streptococcus mutans was allowed to form biofilms on the teeth for two days before treatment procedures were carried out from day 3 to day 6. The relative biofilm viability analyzed by Cell Counting Kit-8 showed that it was significantly lower (at 55.7%) for CMC-ClyR-ACP than seen for ddH 2 O (89.9%), which was consistent with result of confocal laser scanning microscopy.The percentage surface hardness loss of CMC-ClyR-ACP (29.2%) was significantly lower than that of CMC-ACP (51.0%) and ClyR (58.7%) alone, and there was no significant difference between CMC-ClyR-ACP and chlorhexidine (26.9%), which was confirmed by scanning electron microscopy. Therefore, CMC-ClyR-ACP nanogel may be an effective strategy for the control of enamel demineralization.

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Section: Discussionmentioning

confidence: 99%

The dual anti‐caries effect of carboxymethyl chitosan nanogel loaded with chimeric lysin ClyR and amorphous calcium phosphate

Zhu

Yan

Mujtaba

et al. 2021

European J Oral Sciences

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“…The results shown an inhibition of adherence of Streptococcus mutans and Streptococcus gordonii for 90% and 86% respectively, and a reduction of biofilm formation about 45% and 44%. Additionally, CMC/ACP reduced the attachment of Fusobacterium nucleatum (promoter of biofilm development) to streptococcal biofilm by 75% and acting on both zeta potential of the bacterial suspension and cytochrome c-binding bacteria [180]. Regarding the formulation of toothpastes, Achmad et al synthesized a chitosan-based dentifrice (5%) from white shrimp ( Litopenaeusvannamei ) able to reduce the number of colonies of Streptococcus Mutans in the case of early childhood caries.…”

Section: Applications In Cosmeticsmentioning

confidence: 99%

Chitin and Chitosans: Characteristics, Eco-Friendly Processes, and Applications in Cosmetic Science

et al. 2019

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Huge amounts of chitin and chitosans can be found in the biosphere as important constituents of the exoskeleton of many organisms and as waste by worldwide seafood companies. Presently, politicians, environmentalists, and industrialists encourage the use of these marine polysaccharides as a renewable source developed by alternative eco-friendly processes, especially in the production of regular cosmetics. The aim of this review is to outline the physicochemical and biological properties and the different bioextraction methods of chitin and chitosan sources, focusing on enzymatic deproteinization, bacteria fermentation, and enzymatic deacetylation methods. Thanks to their biodegradability, non-toxicity, biocompatibility, and bioactivity, the applications of these marine polymers are widely used in the contemporary manufacturing of biomedical and pharmaceutical products. In the end, advanced cosmetics based on chitin and chitosans are presented, analyzing different therapeutic aspects regarding skin, hair, nail, and oral care. The innovative formulations described can be considered excellent candidates for the prevention and treatment of several diseases associated with different body anatomical sectors.

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“…The cationic amino groups in the nanocomplexes neutralized the anionic bacterial surface charge, decreasing bacterial adhesion to surfaces. The nanocomplexes enhanced the flocculation of particles in the solution, reducing the cell-cell adhesion [176]. Liao et al [177] have manufactured theranostic Au@Ag core-shell NP-decorated silicon nanowires with therapeutic antibacterial properties against S. aureus and E. coli via physical stress and chemical effects.…”

Section: Theranostic Electrospun Nanofibersmentioning

confidence: 99%

Nanotheranostics: A Possible Solution for Drug-Resistant Staphylococcus aureus and their Biofilms?

et al. 2021

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Staphylococcus aureus is a notorious pathogen that colonizes implants (orthopedic and breast implants) and wounds with a vicious resistance to antibiotic therapy. Methicillin-resistant S. aureus (MRSA) is a catastrophe mainly restricted to hospitals and emerged to community reservoirs, acquiring resistance and forming biofilms. Treating biofilms is problematic except via implant removal or wound debridement. Nanoparticles (NPs) and nanofibers could combat superbugs and biofilms and rapidly diagnose MRSA. Nanotheranostics combine diagnostics and therapeutics into a single agent. This comprehensive review is interpretative, utilizing mainly recent literature (since 2016) besides the older remarkable studies sourced via Google Scholar and PubMed. We unravel the molecular S. aureus resistance and complex biofilm. The diagnostic properties and detailed antibacterial and antibiofilm NP mechanisms are elucidated in exciting stories. We highlight the challenges of bacterial infections nanotheranostics. Finally, we discuss the literature and provide “three action appraisals”. (i) The first appraisal consists of preventive actions (two wings), avoiding unnecessary hospital visits, hand hygiene, and legislations against over-the-counter antibiotics as the general preventive wing. Our second recommended preventive wing includes preventing the adverse side effects of the NPs from resistance and toxicity by establishing standard testing procedures. These standard procedures should provide breakpoints of bacteria’s susceptibility to NPs and a thorough toxicological examination of every single batch of synthesized NPs. (ii) The second appraisal includes theranostic actions, using nanotheranostics to diagnose and treat MRSA, such as what we call “multifunctional theranostic nanofibers. (iii) The third action appraisal consists of collaborative actions.

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Nanocomplexes of carboxymethyl chitosan/amorphous calcium phosphate reduce oral bacteria adherence and biofilm formation on human enamel surface

Cited by 34 publications

References 52 publications

The dual anti‐caries effect of carboxymethyl chitosan nanogel loaded with chimeric lysin ClyR and amorphous calcium phosphate

The dual anti‐caries effect of carboxymethyl chitosan nanogel loaded with chimeric lysin ClyR and amorphous calcium phosphate

Chitin and Chitosans: Characteristics, Eco-Friendly Processes, and Applications in Cosmetic Science

Nanotheranostics: A Possible Solution for Drug-Resistant Staphylococcus aureus and their Biofilms?

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