Nanocontainers for Analytical Applications

Hofmann, Carola; Duerkop, Axel; Baeumner, Antje J.

doi:10.1002/anie.201811821

Cited by 55 publications

(45 citation statements)

References 128 publications

(521 reference statements)

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“…Storage and delivery of different compounds for medicinal applications, chemical reactions and analysis, cosmetics, and coatings is of great importance. [1][2][3][4] Biological systems have developed a very useful tool to achieve this. Biological cell membranes can compartmentalize into different sizes (nano to micrometre) of vesicles to protect and transport the different components of life within a physiological medium.…”

Section: Introductionmentioning

confidence: 99%

Self-assembly of reversed bilayer vesicles through pnictogen bonding: water-stable supramolecular nanocontainers for organic solvents

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Self-assembly of reversed bilayer vesicles through pnictogen bonding: water-stable supramolecular nanocontainers for organic solvents

et al. 2020

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“…In contrast to enzyme labels that generate the fluorophores in situ, the encapsulated fluorophores are released on demand from the nanocontainer to avoid self-quenching inside the confined environment. [23] There are also mixed detection schemes,e.g., in the form of electrochemiluminescence that generates astrong signal without background.…”

Section: Introductionmentioning

confidence: 99%

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

et al. 2020

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The ability to detect low concentrations of analytes and in particular low‐abundance biomarkers is of fundamental importance, e.g., for early‐stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single‐molecule bioaffinity assays. While many review articles have highlighted the potentials of single‐molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real‐world applications as one should expect. This Review provides a theoretical background on single‐molecule—or better digital—assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single‐molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.

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“…In all cases, a sufficient amount of label is necessary to achieve the required contrast and sensitivity. Therefore, also signal amplification strategies play an important role which is often achieved using nanomaterials [14][15][16]. In the case of liposomes, this can be achieved via the entrapment of numerous signaling molecules inside the vesicles which leads to a significant increase in the signal upon their release [7,[17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Cationic liposomes for generic signal amplification strategies in bioassays

et al. 2020

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Liposomes have been widely applied in bioanalytical assays. Most liposomes used bare negative charges to prevent non-specific binding and increase colloidal stability. Here, in contrast, highly stable, positively charged liposomes entrapping the fluorescent dye sulforhodamine B (SRB) were developed to serve as a secondary, non-specific label‚ and signal amplification tool in bioanalytical systems by exploiting their electrostatic interaction with negatively charged vesicles, surfaces, and microorganisms. The cationic liposomes were optimized for long-term stability (> 5 months) and high dye entrapment yield. Their capability as secondary, non-specific labels was first successfully proven through electrostatic interactions of cationic and anionic liposomes using dynamic light scattering, and then in a bioassay with fluorescence detection leading to an enhancement factor of 8.5 without any additional surface blocking steps. Moreover, the cationic liposomes bound efficiently to anionic magnetic beads were stable throughout magnetic separation procedures and could hence serve directly as labels in magnetic separation and purification strategies. Finally, the electrostatic interaction was exploited for the direct, simple, non-specific labeling of gram-negative bacteria. Isolated Escherichia coli cells were chosen as models and direct detection was demonstrated via fluorescent and chemiluminescent liposomes. Thus, these cationic liposomes can be used as generic labels for the development of ultrasensitive bioassays based on electrostatic interaction without the need for additional expensive recognition units like antibodies, where desired specificity is already afforded through other strategies.

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Nanocontainers for Analytical Applications

Cited by 55 publications

References 128 publications

Self-assembly of reversed bilayer vesicles through pnictogen bonding: water-stable supramolecular nanocontainers for organic solvents

Self-assembly of reversed bilayer vesicles through pnictogen bonding: water-stable supramolecular nanocontainers for organic solvents

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

Cationic liposomes for generic signal amplification strategies in bioassays

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